Promoter Architecture and Promoter Engineering in <i>Saccharomyces cerevisiae</i>
Promoters play an essential role in the regulation of gene expression for fine-tuning genetic circuits and metabolic pathways in <i>Saccharomyces cerevisiae</i> (<i>S. cerevisiae</i>). However, native promoters in <i>S. cerevisiae</i> have several limitations whic...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2020-08-01
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| Series: | Metabolites |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2218-1989/10/8/320 |
| _version_ | 1827711336118747136 |
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| author | Hongting Tang Yanling Wu Jiliang Deng Nanzhu Chen Zhaohui Zheng Yongjun Wei Xiaozhou Luo Jay D. Keasling |
| author_facet | Hongting Tang Yanling Wu Jiliang Deng Nanzhu Chen Zhaohui Zheng Yongjun Wei Xiaozhou Luo Jay D. Keasling |
| author_sort | Hongting Tang |
| collection | DOAJ |
| description | Promoters play an essential role in the regulation of gene expression for fine-tuning genetic circuits and metabolic pathways in <i>Saccharomyces cerevisiae</i> (<i>S. cerevisiae</i>). However, native promoters in <i>S. cerevisiae</i> have several limitations which hinder their applications in metabolic engineering. These limitations include an inadequate number of well-characterized promoters, poor dynamic range, and insufficient orthogonality to endogenous regulations. Therefore, it is necessary to perform promoter engineering to create synthetic promoters with better properties. Here, we review recent advances related to promoter architecture, promoter engineering and synthetic promoter applications in <i>S. cerevisiae</i>. We also provide a perspective of future directions in this field with an emphasis on the recent advances of machine learning based promoter designs. |
| first_indexed | 2024-03-10T17:53:13Z |
| format | Article |
| id | doaj.art-baeb786d00a34f0c836e7da21e57f32c |
| institution | Directory Open Access Journal |
| issn | 2218-1989 |
| language | English |
| last_indexed | 2024-03-10T17:53:13Z |
| publishDate | 2020-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Metabolites |
| spelling | doaj.art-baeb786d00a34f0c836e7da21e57f32c2023-11-20T09:18:39ZengMDPI AGMetabolites2218-19892020-08-0110832010.3390/metabo10080320Promoter Architecture and Promoter Engineering in <i>Saccharomyces cerevisiae</i>Hongting Tang0Yanling Wu1Jiliang Deng2Nanzhu Chen3Zhaohui Zheng4Yongjun Wei5Xiaozhou Luo6Jay D. Keasling7Center for Synthetic Biochemistry, Shenzhen Institutes for Advanced Technologies, Chinese Academy of Sciences, Shenzhen 518055, ChinaCenter for Synthetic Biochemistry, Shenzhen Institutes for Advanced Technologies, Chinese Academy of Sciences, Shenzhen 518055, ChinaCenter for Synthetic Biochemistry, Shenzhen Institutes for Advanced Technologies, Chinese Academy of Sciences, Shenzhen 518055, ChinaCenter for Synthetic Biochemistry, Shenzhen Institutes for Advanced Technologies, Chinese Academy of Sciences, Shenzhen 518055, ChinaCenter for Synthetic Biochemistry, Shenzhen Institutes for Advanced Technologies, Chinese Academy of Sciences, Shenzhen 518055, ChinaSchool of Pharmaceutical Sciences, Key Laboratory of Advanced Drug Preparation Technologies, Ministry of Education, Zhengzhou University, Zhengzhou 450001, ChinaCenter for Synthetic Biochemistry, Shenzhen Institutes for Advanced Technologies, Chinese Academy of Sciences, Shenzhen 518055, ChinaCenter for Synthetic Biochemistry, Shenzhen Institutes for Advanced Technologies, Chinese Academy of Sciences, Shenzhen 518055, ChinaPromoters play an essential role in the regulation of gene expression for fine-tuning genetic circuits and metabolic pathways in <i>Saccharomyces cerevisiae</i> (<i>S. cerevisiae</i>). However, native promoters in <i>S. cerevisiae</i> have several limitations which hinder their applications in metabolic engineering. These limitations include an inadequate number of well-characterized promoters, poor dynamic range, and insufficient orthogonality to endogenous regulations. Therefore, it is necessary to perform promoter engineering to create synthetic promoters with better properties. Here, we review recent advances related to promoter architecture, promoter engineering and synthetic promoter applications in <i>S. cerevisiae</i>. We also provide a perspective of future directions in this field with an emphasis on the recent advances of machine learning based promoter designs.https://www.mdpi.com/2218-1989/10/8/320promoter architecturepromoter engineeringsynthetic promotersynthetic biologymachine learning<i>Saccharomyces cerevisiae</i> |
| spellingShingle | Hongting Tang Yanling Wu Jiliang Deng Nanzhu Chen Zhaohui Zheng Yongjun Wei Xiaozhou Luo Jay D. Keasling Promoter Architecture and Promoter Engineering in <i>Saccharomyces cerevisiae</i> Metabolites promoter architecture promoter engineering synthetic promoter synthetic biology machine learning <i>Saccharomyces cerevisiae</i> |
| title | Promoter Architecture and Promoter Engineering in <i>Saccharomyces cerevisiae</i> |
| title_full | Promoter Architecture and Promoter Engineering in <i>Saccharomyces cerevisiae</i> |
| title_fullStr | Promoter Architecture and Promoter Engineering in <i>Saccharomyces cerevisiae</i> |
| title_full_unstemmed | Promoter Architecture and Promoter Engineering in <i>Saccharomyces cerevisiae</i> |
| title_short | Promoter Architecture and Promoter Engineering in <i>Saccharomyces cerevisiae</i> |
| title_sort | promoter architecture and promoter engineering in i saccharomyces cerevisiae i |
| topic | promoter architecture promoter engineering synthetic promoter synthetic biology machine learning <i>Saccharomyces cerevisiae</i> |
| url | https://www.mdpi.com/2218-1989/10/8/320 |
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