Dermal White Adipose Tissue (dWAT) Is Regulated by Foxn1 and Hif-1α during the Early Phase of Skin Wound Healing
Dermal white adipose tissue (dWAT) is involved in the maintenance of skin homeostasis. However, the studies concerning its molecular regulation are limited. In the present paper, we ask whether the introduction of two transcription factors, Foxn1 and Hif-1α, into the post-wounded skin of Foxn1<su...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2021-12-01
|
| Series: | International Journal of Molecular Sciences |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1422-0067/23/1/257 |
| _version_ | 1797498828962660352 |
|---|---|
| author | Barbara Gawronska-Kozak Katarzyna Walendzik Sylwia Machcinska Artur Padzik Marta Kopcewicz Joanna Wiśniewska |
| author_facet | Barbara Gawronska-Kozak Katarzyna Walendzik Sylwia Machcinska Artur Padzik Marta Kopcewicz Joanna Wiśniewska |
| author_sort | Barbara Gawronska-Kozak |
| collection | DOAJ |
| description | Dermal white adipose tissue (dWAT) is involved in the maintenance of skin homeostasis. However, the studies concerning its molecular regulation are limited. In the present paper, we ask whether the introduction of two transcription factors, Foxn1 and Hif-1α, into the post-wounded skin of Foxn1<sup>−/−</sup> mice regulates dWAT during wound healing (days 3 and 6). We have chosen lentivirus vectors (LVs) as a tool to deliver Foxn1 and Hif-1α into the post-wounded skin. We documented that combinations of both transgenes reduces the number, size and diameter of dermal adipocytes at the wound bed area. The qRT-PCR analysis of pro-adipogenic genes, revealed that LV-Hif-1α alone, or combined with LV-Foxn1, increases the mRNA expression of <i>Pparγ</i>, <i>Glut 4</i> and <i>Fasn</i> at post-wounding day 6. However, the most spectacular stimulatory effect of Foxn1 and/or Hif-1α was observed for Igf2, the growth factor participating in adipogenic signal transduction. Our data also shows that Foxn1/Hif-1α, at post-wounding day 3, reduces levels of <i>CD68</i> and <i>MIP-1γ</i> mRNA expression and the percentage of CD68 positive cells in the wound site. In conclusion, the present data are the first to document that Foxn1 and Hif-1α cooperatively (1) regulate dWAT during the proliferative phase of skin wound healing through the Igf2 signaling pathway, and (2) reduce the macrophages content in the wound site. |
| first_indexed | 2024-03-10T03:39:55Z |
| format | Article |
| id | doaj.art-baec68f930ad4d96848bd30c81539111 |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-10T03:39:55Z |
| publishDate | 2021-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-baec68f930ad4d96848bd30c815391112023-11-23T11:37:14ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672021-12-0123125710.3390/ijms23010257Dermal White Adipose Tissue (dWAT) Is Regulated by Foxn1 and Hif-1α during the Early Phase of Skin Wound HealingBarbara Gawronska-Kozak0Katarzyna Walendzik1Sylwia Machcinska2Artur Padzik3Marta Kopcewicz4Joanna Wiśniewska5Institute of Animal Reproduction and Food Research, Polish Academy of Sciences, 10-748 Olsztyn, PolandInstitute of Animal Reproduction and Food Research, Polish Academy of Sciences, 10-748 Olsztyn, PolandInstitute of Animal Reproduction and Food Research, Polish Academy of Sciences, 10-748 Olsztyn, PolandVirus Vector Core, Turku Centre for Biotechnology BioCity, 20520 Turku, FinlandInstitute of Animal Reproduction and Food Research, Polish Academy of Sciences, 10-748 Olsztyn, PolandInstitute of Animal Reproduction and Food Research, Polish Academy of Sciences, 10-748 Olsztyn, PolandDermal white adipose tissue (dWAT) is involved in the maintenance of skin homeostasis. However, the studies concerning its molecular regulation are limited. In the present paper, we ask whether the introduction of two transcription factors, Foxn1 and Hif-1α, into the post-wounded skin of Foxn1<sup>−/−</sup> mice regulates dWAT during wound healing (days 3 and 6). We have chosen lentivirus vectors (LVs) as a tool to deliver Foxn1 and Hif-1α into the post-wounded skin. We documented that combinations of both transgenes reduces the number, size and diameter of dermal adipocytes at the wound bed area. The qRT-PCR analysis of pro-adipogenic genes, revealed that LV-Hif-1α alone, or combined with LV-Foxn1, increases the mRNA expression of <i>Pparγ</i>, <i>Glut 4</i> and <i>Fasn</i> at post-wounding day 6. However, the most spectacular stimulatory effect of Foxn1 and/or Hif-1α was observed for Igf2, the growth factor participating in adipogenic signal transduction. Our data also shows that Foxn1/Hif-1α, at post-wounding day 3, reduces levels of <i>CD68</i> and <i>MIP-1γ</i> mRNA expression and the percentage of CD68 positive cells in the wound site. In conclusion, the present data are the first to document that Foxn1 and Hif-1α cooperatively (1) regulate dWAT during the proliferative phase of skin wound healing through the Igf2 signaling pathway, and (2) reduce the macrophages content in the wound site.https://www.mdpi.com/1422-0067/23/1/257skinwound healingdermal white adipose tissueFoxn1Hif-1α |
| spellingShingle | Barbara Gawronska-Kozak Katarzyna Walendzik Sylwia Machcinska Artur Padzik Marta Kopcewicz Joanna Wiśniewska Dermal White Adipose Tissue (dWAT) Is Regulated by Foxn1 and Hif-1α during the Early Phase of Skin Wound Healing International Journal of Molecular Sciences skin wound healing dermal white adipose tissue Foxn1 Hif-1α |
| title | Dermal White Adipose Tissue (dWAT) Is Regulated by Foxn1 and Hif-1α during the Early Phase of Skin Wound Healing |
| title_full | Dermal White Adipose Tissue (dWAT) Is Regulated by Foxn1 and Hif-1α during the Early Phase of Skin Wound Healing |
| title_fullStr | Dermal White Adipose Tissue (dWAT) Is Regulated by Foxn1 and Hif-1α during the Early Phase of Skin Wound Healing |
| title_full_unstemmed | Dermal White Adipose Tissue (dWAT) Is Regulated by Foxn1 and Hif-1α during the Early Phase of Skin Wound Healing |
| title_short | Dermal White Adipose Tissue (dWAT) Is Regulated by Foxn1 and Hif-1α during the Early Phase of Skin Wound Healing |
| title_sort | dermal white adipose tissue dwat is regulated by foxn1 and hif 1α during the early phase of skin wound healing |
| topic | skin wound healing dermal white adipose tissue Foxn1 Hif-1α |
| url | https://www.mdpi.com/1422-0067/23/1/257 |
| work_keys_str_mv | AT barbaragawronskakozak dermalwhiteadiposetissuedwatisregulatedbyfoxn1andhif1aduringtheearlyphaseofskinwoundhealing AT katarzynawalendzik dermalwhiteadiposetissuedwatisregulatedbyfoxn1andhif1aduringtheearlyphaseofskinwoundhealing AT sylwiamachcinska dermalwhiteadiposetissuedwatisregulatedbyfoxn1andhif1aduringtheearlyphaseofskinwoundhealing AT arturpadzik dermalwhiteadiposetissuedwatisregulatedbyfoxn1andhif1aduringtheearlyphaseofskinwoundhealing AT martakopcewicz dermalwhiteadiposetissuedwatisregulatedbyfoxn1andhif1aduringtheearlyphaseofskinwoundhealing AT joannawisniewska dermalwhiteadiposetissuedwatisregulatedbyfoxn1andhif1aduringtheearlyphaseofskinwoundhealing |