The Effects of Chia Defatted Flour as a Nutritional Supplement in C57BL/6 Mice Fed a Low-Quality Diet
Today, consumption of diets rich in saturated fat and fructose, associated with a variety of metabolic deregulations, has increased. The aim of this study was to evaluate the effect of dietary supplementation with a residue of defatted chia seed on a diet with low nutritional quality. To do this, C5...
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| Format: | Article |
| Language: | English |
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MDPI AG
2024-02-01
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| Series: | Foods |
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| Online Access: | https://www.mdpi.com/2304-8158/13/5/678 |
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| author | Agustin Lucini Mas Alejandra Mariel Canalis María Eugenia Pasqualini Daniel Alberto Wunderlin María Verónica Baroni |
| author_facet | Agustin Lucini Mas Alejandra Mariel Canalis María Eugenia Pasqualini Daniel Alberto Wunderlin María Verónica Baroni |
| author_sort | Agustin Lucini Mas |
| collection | DOAJ |
| description | Today, consumption of diets rich in saturated fat and fructose, associated with a variety of metabolic deregulations, has increased. The aim of this study was to evaluate the effect of dietary supplementation with a residue of defatted chia seed on a diet with low nutritional quality. To do this, C57BL/6 male mice were fed with the Control (C), Low-Nutritional-Quality (LNQ), or supplemented-with-chia-defatted-flour (LNQ+C) diets. After 12 weeks, the glucose and lactate levels were determined in the serum, liver, and kidney, along with reactive oxygen species (ROS) levels, antioxidant enzyme activity, reduced glutathione (GSH), and protein oxidation (AOPP). The LNQ diet increased the glucose and lactate levels (+25% and +50% approx. in the liver, with respect to the control group) and generated oxidative stress by modifying the levels of ROS and the activity of antioxidant enzymes, causing oxidative damage to proteins (+12% in the liver, with respect to the control). Chia supplementation helped to restore the glucose to control levels and modulate the endogenous antioxidant system, resulting in a decrease in protein oxidation products with no differences compared to the control group. In conclusion, supplementation with chia showed beneficial effects on the general health of mice, even when fed a low-nutritional-quality diet. |
| first_indexed | 2024-04-25T00:30:22Z |
| format | Article |
| id | doaj.art-baec713651d74fc385f182bd1d2ce414 |
| institution | Directory Open Access Journal |
| issn | 2304-8158 |
| language | English |
| last_indexed | 2024-04-25T00:30:22Z |
| publishDate | 2024-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Foods |
| spelling | doaj.art-baec713651d74fc385f182bd1d2ce4142024-03-12T16:44:05ZengMDPI AGFoods2304-81582024-02-0113567810.3390/foods13050678The Effects of Chia Defatted Flour as a Nutritional Supplement in C57BL/6 Mice Fed a Low-Quality DietAgustin Lucini Mas0Alejandra Mariel Canalis1María Eugenia Pasqualini2Daniel Alberto Wunderlin3María Verónica Baroni4Instituto de Ciencia y Tecnología de Alimentos Córdoba (ICYTAC-CONICET), SeCyT—Universidad Nacional de Córdoba, Córdoba X5000GYA, ArgentinaInstituto de Ciencia y Tecnología de Alimentos Córdoba (ICYTAC-CONICET), SeCyT—Universidad Nacional de Córdoba, Córdoba X5000GYA, ArgentinaInstituto de Investigaciones en Ciencias de la Salud (INICSA-CONICET), Pabellón Biología Celular, Universidad Nacional de Córdoba, Córdoba X5000GYA, ArgentinaInstituto de Ciencia y Tecnología de Alimentos Córdoba (ICYTAC-CONICET), SeCyT—Universidad Nacional de Córdoba, Córdoba X5000GYA, ArgentinaInstituto de Ciencia y Tecnología de Alimentos Córdoba (ICYTAC-CONICET), SeCyT—Universidad Nacional de Córdoba, Córdoba X5000GYA, ArgentinaToday, consumption of diets rich in saturated fat and fructose, associated with a variety of metabolic deregulations, has increased. The aim of this study was to evaluate the effect of dietary supplementation with a residue of defatted chia seed on a diet with low nutritional quality. To do this, C57BL/6 male mice were fed with the Control (C), Low-Nutritional-Quality (LNQ), or supplemented-with-chia-defatted-flour (LNQ+C) diets. After 12 weeks, the glucose and lactate levels were determined in the serum, liver, and kidney, along with reactive oxygen species (ROS) levels, antioxidant enzyme activity, reduced glutathione (GSH), and protein oxidation (AOPP). The LNQ diet increased the glucose and lactate levels (+25% and +50% approx. in the liver, with respect to the control group) and generated oxidative stress by modifying the levels of ROS and the activity of antioxidant enzymes, causing oxidative damage to proteins (+12% in the liver, with respect to the control). Chia supplementation helped to restore the glucose to control levels and modulate the endogenous antioxidant system, resulting in a decrease in protein oxidation products with no differences compared to the control group. In conclusion, supplementation with chia showed beneficial effects on the general health of mice, even when fed a low-nutritional-quality diet.https://www.mdpi.com/2304-8158/13/5/678oxidative stresschiaglucose metabolismpolyphenolsantioxidanthigh fructose |
| spellingShingle | Agustin Lucini Mas Alejandra Mariel Canalis María Eugenia Pasqualini Daniel Alberto Wunderlin María Verónica Baroni The Effects of Chia Defatted Flour as a Nutritional Supplement in C57BL/6 Mice Fed a Low-Quality Diet Foods oxidative stress chia glucose metabolism polyphenols antioxidant high fructose |
| title | The Effects of Chia Defatted Flour as a Nutritional Supplement in C57BL/6 Mice Fed a Low-Quality Diet |
| title_full | The Effects of Chia Defatted Flour as a Nutritional Supplement in C57BL/6 Mice Fed a Low-Quality Diet |
| title_fullStr | The Effects of Chia Defatted Flour as a Nutritional Supplement in C57BL/6 Mice Fed a Low-Quality Diet |
| title_full_unstemmed | The Effects of Chia Defatted Flour as a Nutritional Supplement in C57BL/6 Mice Fed a Low-Quality Diet |
| title_short | The Effects of Chia Defatted Flour as a Nutritional Supplement in C57BL/6 Mice Fed a Low-Quality Diet |
| title_sort | effects of chia defatted flour as a nutritional supplement in c57bl 6 mice fed a low quality diet |
| topic | oxidative stress chia glucose metabolism polyphenols antioxidant high fructose |
| url | https://www.mdpi.com/2304-8158/13/5/678 |
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