Protocol for isolation and analysis of the leukemia stem cells in BCR-ABL-driven chronic myelogenous leukemia mice
Summary: Practical procedures for sorting and analysis of leukemia stem cells (LSCs) are to improve our understanding of chronic myelogenous leukemia (CML). Here, we present a detailed magnetic-bead-based sorting and flow-cytometry-based analysis protocol for LSCs in BCR-ABL-driven CML mice. We desc...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2023-03-01
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| Series: | STAR Protocols |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666166723000813 |
| _version_ | 1797905349169119232 |
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| author | Chang Liu Xiaoying Lin Yanli Jin Jingxuan Pan |
| author_facet | Chang Liu Xiaoying Lin Yanli Jin Jingxuan Pan |
| author_sort | Chang Liu |
| collection | DOAJ |
| description | Summary: Practical procedures for sorting and analysis of leukemia stem cells (LSCs) are to improve our understanding of chronic myelogenous leukemia (CML). Here, we present a detailed magnetic-bead-based sorting and flow-cytometry-based analysis protocol for LSCs in BCR-ABL-driven CML mice. We describe steps for sorting and functional analysis of BCR-ABL-expressing c-Kit+ cells (GFP+c-Kit+) from CML mice as well as antibody staining and gating strategies for characterization of leukemia stem/progenitor cells and myeloid leukemia cells.For complete details on the use and execution of this protocol, please refer to Liu et al. (2022).1 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics. |
| first_indexed | 2024-04-10T10:03:53Z |
| format | Article |
| id | doaj.art-baed0beee0d84956ac278256a99e7b46 |
| institution | Directory Open Access Journal |
| issn | 2666-1667 |
| language | English |
| last_indexed | 2024-04-10T10:03:53Z |
| publishDate | 2023-03-01 |
| publisher | Elsevier |
| record_format | Article |
| series | STAR Protocols |
| spelling | doaj.art-baed0beee0d84956ac278256a99e7b462023-02-16T04:18:50ZengElsevierSTAR Protocols2666-16672023-03-0141102123Protocol for isolation and analysis of the leukemia stem cells in BCR-ABL-driven chronic myelogenous leukemia miceChang Liu0Xiaoying Lin1Yanli Jin2Jingxuan Pan3Jinan University Institute of Tumor Pharmacology, College of Pharmacy, Jinan University, Guangzhou 510632, ChinaJinan University Institute of Tumor Pharmacology, College of Pharmacy, Jinan University, Guangzhou 510632, ChinaJinan University Institute of Tumor Pharmacology, College of Pharmacy, Jinan University, Guangzhou 510632, China; Corresponding authorState Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-sen University, Guangzhou 510060, China; Corresponding authorSummary: Practical procedures for sorting and analysis of leukemia stem cells (LSCs) are to improve our understanding of chronic myelogenous leukemia (CML). Here, we present a detailed magnetic-bead-based sorting and flow-cytometry-based analysis protocol for LSCs in BCR-ABL-driven CML mice. We describe steps for sorting and functional analysis of BCR-ABL-expressing c-Kit+ cells (GFP+c-Kit+) from CML mice as well as antibody staining and gating strategies for characterization of leukemia stem/progenitor cells and myeloid leukemia cells.For complete details on the use and execution of this protocol, please refer to Liu et al. (2022).1 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.http://www.sciencedirect.com/science/article/pii/S2666166723000813Cell isolationFlow Cytometry/Mass CytometryModel OrganismsStem Cells |
| spellingShingle | Chang Liu Xiaoying Lin Yanli Jin Jingxuan Pan Protocol for isolation and analysis of the leukemia stem cells in BCR-ABL-driven chronic myelogenous leukemia mice STAR Protocols Cell isolation Flow Cytometry/Mass Cytometry Model Organisms Stem Cells |
| title | Protocol for isolation and analysis of the leukemia stem cells in BCR-ABL-driven chronic myelogenous leukemia mice |
| title_full | Protocol for isolation and analysis of the leukemia stem cells in BCR-ABL-driven chronic myelogenous leukemia mice |
| title_fullStr | Protocol for isolation and analysis of the leukemia stem cells in BCR-ABL-driven chronic myelogenous leukemia mice |
| title_full_unstemmed | Protocol for isolation and analysis of the leukemia stem cells in BCR-ABL-driven chronic myelogenous leukemia mice |
| title_short | Protocol for isolation and analysis of the leukemia stem cells in BCR-ABL-driven chronic myelogenous leukemia mice |
| title_sort | protocol for isolation and analysis of the leukemia stem cells in bcr abl driven chronic myelogenous leukemia mice |
| topic | Cell isolation Flow Cytometry/Mass Cytometry Model Organisms Stem Cells |
| url | http://www.sciencedirect.com/science/article/pii/S2666166723000813 |
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