A Longitudinal Epidemiology Study of Meningococcal Carriage in Students 13 to 25 Years Old in Quebec
ABSTRACT Neisseria meningitidis carriage data are necessary to inform serogroup B (NmB) immunization program implementation. This longitudinal study compared detection methods to measure N. meningitidis throat carriage prevalence in Quebec from November 2010 to December 2013 using cultured swab isol...
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| Format: | Article |
| Language: | English |
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American Society for Microbiology
2018-12-01
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| Series: | mSphere |
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| Online Access: | https://journals.asm.org/doi/10.1128/mSphere.00427-18 |
| _version_ | 1828977133694746624 |
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| author | Rodica Gilca Philippe De Wals Sheila M. Nolan Nicholas Kitchin Joseph J. Eiden Qin Jiang C. Hal Jones Kathrin U. Jansen Annaliesa S. Anderson Louise Pedneault |
| author_facet | Rodica Gilca Philippe De Wals Sheila M. Nolan Nicholas Kitchin Joseph J. Eiden Qin Jiang C. Hal Jones Kathrin U. Jansen Annaliesa S. Anderson Louise Pedneault |
| author_sort | Rodica Gilca |
| collection | DOAJ |
| description | ABSTRACT Neisseria meningitidis carriage data are necessary to inform serogroup B (NmB) immunization program implementation. This longitudinal study compared detection methods to measure N. meningitidis throat carriage prevalence in Quebec from November 2010 to December 2013 using cultured swab isolates and direct swab PCR from students in ninth grade (aged 13 to 15 years; n = 534) and eleventh grade/college entry (16 to 18 years; n = 363) and in university students in dormitories (18 to 25 years; n = 360) at 3 time points per group. Meningococcal and NmB carriage rates were lower in ninth- and eleventh-grade/college entry students than university students, regardless of methodology. Genotyping cultured isolates by PCR detected NmB and non-NmB in 2.1% and 7.3% of ninth-grade students, in 1.7% and 7.2% of eleventh-grade/college entry students, and in 7.5% and 21.9% of university students, respectively. NmB acquisition rates were 1.9, 0.7, and 3.3 per 1,000 person-months across respective age groups. Most NmB isolates (94.7%, 76.9%, and 86.8%, respectively) expressed subfamily A factor H binding-protein (fHBP) variants. The most common non-NmB serogroups were NmY (1.7%/1.1%) from ninth grade and eleventh grade/college entry and NmW (2.8%) from university students. Genomic analyses detected disease-associated sequence types in carriage isolates, and carriage could persist for months. This is the largest longitudinal carriage study in Canada and the first to report fHBP variants in NmB carriage isolates in healthy Canadians. These data contribute to identification of the optimal window for NmB vaccination in precollege adolescents and provide a baseline for investigating NmB vaccination effects on carriage in this population. IMPORTANCE Disease caused by Neisseria meningitidis is associated with serious complications and a high fatality rate. Asymptomatic individuals can harbor the bacterium in the throat, a state known as “carriage,” which can lead to person-to-person spread of the pathogen. This study examined N. meningitidis carriage from 2010 to 2013 among 2 groups in the Quebec City region: ninth-grade students (aged 13 to 15 years), who were also followed in their last year of high school (eleventh grade/college entry; 16 to 18 years), and university students (18 to 25 years); both groups have been shown in some other geographic regions to have high rates of carriage. This study demonstrated that N. meningitidis carriage rates were higher among university students in dormitories than ninth-grade and eleventh-grade/college entry students. Understanding carriage rates in these age groups leads to better strategies to control N. meningitidis by targeting vaccination to those responsible for transmission within the population. |
| first_indexed | 2024-12-14T14:52:46Z |
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| institution | Directory Open Access Journal |
| issn | 2379-5042 |
| language | English |
| last_indexed | 2024-12-14T14:52:46Z |
| publishDate | 2018-12-01 |
| publisher | American Society for Microbiology |
| record_format | Article |
| series | mSphere |
| spelling | doaj.art-baed79086a814f6b96fec02338df90942022-12-21T22:57:06ZengAmerican Society for MicrobiologymSphere2379-50422018-12-013610.1128/mSphere.00427-18A Longitudinal Epidemiology Study of Meningococcal Carriage in Students 13 to 25 Years Old in QuebecRodica Gilca0Philippe De Wals1Sheila M. Nolan2Nicholas Kitchin3Joseph J. Eiden4Qin Jiang5C. Hal Jones6Kathrin U. Jansen7Annaliesa S. Anderson8Louise Pedneault9Research Center, Centre Hospitalier Universitaire de Québec-Université Laval, Québec City, Quebec, CanadaResearch Center, Centre Hospitalier Universitaire de Québec-Université Laval, Québec City, Quebec, CanadaVaccine Clinical Research and Development, Pfizer Inc., Pearl River, New York, USAVaccine Clinical Research and Development, Pfizer Ltd., Hurley, Berkshire, United KingdomVaccine Clinical Research and Development, Pfizer Inc., Pearl River, New York, USAVaccine Clinical Research and Development, Pfizer Inc., Collegeville, Pennsylvania, USAVaccine Research and Development, Pfizer Inc., Pearl River, New York, USAVaccine Research and Development, Pfizer Inc., Pearl River, New York, USAVaccine Research and Development, Pfizer Inc., Pearl River, New York, USAVaccine Clinical Research and Development, Pfizer Inc., Pearl River, New York, USAABSTRACT Neisseria meningitidis carriage data are necessary to inform serogroup B (NmB) immunization program implementation. This longitudinal study compared detection methods to measure N. meningitidis throat carriage prevalence in Quebec from November 2010 to December 2013 using cultured swab isolates and direct swab PCR from students in ninth grade (aged 13 to 15 years; n = 534) and eleventh grade/college entry (16 to 18 years; n = 363) and in university students in dormitories (18 to 25 years; n = 360) at 3 time points per group. Meningococcal and NmB carriage rates were lower in ninth- and eleventh-grade/college entry students than university students, regardless of methodology. Genotyping cultured isolates by PCR detected NmB and non-NmB in 2.1% and 7.3% of ninth-grade students, in 1.7% and 7.2% of eleventh-grade/college entry students, and in 7.5% and 21.9% of university students, respectively. NmB acquisition rates were 1.9, 0.7, and 3.3 per 1,000 person-months across respective age groups. Most NmB isolates (94.7%, 76.9%, and 86.8%, respectively) expressed subfamily A factor H binding-protein (fHBP) variants. The most common non-NmB serogroups were NmY (1.7%/1.1%) from ninth grade and eleventh grade/college entry and NmW (2.8%) from university students. Genomic analyses detected disease-associated sequence types in carriage isolates, and carriage could persist for months. This is the largest longitudinal carriage study in Canada and the first to report fHBP variants in NmB carriage isolates in healthy Canadians. These data contribute to identification of the optimal window for NmB vaccination in precollege adolescents and provide a baseline for investigating NmB vaccination effects on carriage in this population. IMPORTANCE Disease caused by Neisseria meningitidis is associated with serious complications and a high fatality rate. Asymptomatic individuals can harbor the bacterium in the throat, a state known as “carriage,” which can lead to person-to-person spread of the pathogen. This study examined N. meningitidis carriage from 2010 to 2013 among 2 groups in the Quebec City region: ninth-grade students (aged 13 to 15 years), who were also followed in their last year of high school (eleventh grade/college entry; 16 to 18 years), and university students (18 to 25 years); both groups have been shown in some other geographic regions to have high rates of carriage. This study demonstrated that N. meningitidis carriage rates were higher among university students in dormitories than ninth-grade and eleventh-grade/college entry students. Understanding carriage rates in these age groups leads to better strategies to control N. meningitidis by targeting vaccination to those responsible for transmission within the population.https://journals.asm.org/doi/10.1128/mSphere.00427-18Neisseria meningitidisNmBepidemiologyfactor H binding proteinoropharyngeal carriageserogroup B |
| spellingShingle | Rodica Gilca Philippe De Wals Sheila M. Nolan Nicholas Kitchin Joseph J. Eiden Qin Jiang C. Hal Jones Kathrin U. Jansen Annaliesa S. Anderson Louise Pedneault A Longitudinal Epidemiology Study of Meningococcal Carriage in Students 13 to 25 Years Old in Quebec mSphere Neisseria meningitidis NmB epidemiology factor H binding protein oropharyngeal carriage serogroup B |
| title | A Longitudinal Epidemiology Study of Meningococcal Carriage in Students 13 to 25 Years Old in Quebec |
| title_full | A Longitudinal Epidemiology Study of Meningococcal Carriage in Students 13 to 25 Years Old in Quebec |
| title_fullStr | A Longitudinal Epidemiology Study of Meningococcal Carriage in Students 13 to 25 Years Old in Quebec |
| title_full_unstemmed | A Longitudinal Epidemiology Study of Meningococcal Carriage in Students 13 to 25 Years Old in Quebec |
| title_short | A Longitudinal Epidemiology Study of Meningococcal Carriage in Students 13 to 25 Years Old in Quebec |
| title_sort | longitudinal epidemiology study of meningococcal carriage in students 13 to 25 years old in quebec |
| topic | Neisseria meningitidis NmB epidemiology factor H binding protein oropharyngeal carriage serogroup B |
| url | https://journals.asm.org/doi/10.1128/mSphere.00427-18 |
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