UPLC-qTOF-MS Phytochemical Profile and Antiulcer Potential of <i>Cyperus conglomeratus</i> Rottb. Alcoholic Extract
<i>Cyperus</i> has been commonly used as a multi-use medicinal plant in folk medicine worldwide. The objectives of our study were to determine the different metabolites in the <i>Cyperus conglomeratus</i> Rottb. methanol extract, and to assess its in vivo gastroprotective eff...
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| Format: | Article |
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MDPI AG
2020-09-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/25/18/4234 |
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| author | Abdelsamed I. Elshamy Abdel Razik H. Farrag Iriny M. Ayoub Karam A. Mahdy Rehab F. Taher Abd El-Nasser G. EI Gendy Tarik A. Mohamed Salim S. Al-Rejaie Yasser A. EI-Amier Ahmed M. Abd-EIGawad Mohamed A. Farag |
| author_facet | Abdelsamed I. Elshamy Abdel Razik H. Farrag Iriny M. Ayoub Karam A. Mahdy Rehab F. Taher Abd El-Nasser G. EI Gendy Tarik A. Mohamed Salim S. Al-Rejaie Yasser A. EI-Amier Ahmed M. Abd-EIGawad Mohamed A. Farag |
| author_sort | Abdelsamed I. Elshamy |
| collection | DOAJ |
| description | <i>Cyperus</i> has been commonly used as a multi-use medicinal plant in folk medicine worldwide. The objectives of our study were to determine the different metabolites in the <i>Cyperus conglomeratus</i> Rottb. methanol extract, and to assess its in vivo gastroprotective effect in ethanol-induced gastric ulcer model in rats. Serum levels of galactin-3 and TNF-<i>α</i> were employed as biochemical markers. To pinpoint for active agents, comprehensive metabolites profiling of extract via UPLC-qTOF-MS/MS was employed. A total of 77 chromatographic peaks were detected, of which 70 were annotated. The detected metabolites were categorized into phenolic acids and their derivatives, flavonoids, stilbenes, aurones, quinones, terpenes, and steroids. Rats were divided into six groups; healthy control, ulcer control, standard drug group, and 25, 50, 100 mg/kg of <i>C. conglomeratus</i> treated rats. Pre-treatment with <i>C. conglomeratus</i> alcohol extract significantly reduced galactin-3, and TNF-<i>α</i> in ethanol-induced ulcer model at 25, 50, and 100 mg/kg. Further histopathological and histochemical studies revealed moderate erosion of superficial epithelium, few infiltrated inflammatory cells, and depletion of gastric tissue glycoprotein in the ulcer group. Treatment with the extract protected the gastric epithelial cells in a dose-dependent manner. It could be concluded that <i>C. conglomeratus</i> extract provides significant gastroprotective activity in ethanol-induced gastric ulcer and ought to be included in nutraceuticals in the future for ulcer treatment. |
| first_indexed | 2024-03-10T16:18:55Z |
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| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-10T16:18:55Z |
| publishDate | 2020-09-01 |
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| series | Molecules |
| spelling | doaj.art-baeda79b054b4e48bb43cddae6fb5d162023-11-20T13:49:33ZengMDPI AGMolecules1420-30492020-09-012518423410.3390/molecules25184234UPLC-qTOF-MS Phytochemical Profile and Antiulcer Potential of <i>Cyperus conglomeratus</i> Rottb. Alcoholic ExtractAbdelsamed I. Elshamy0Abdel Razik H. Farrag1Iriny M. Ayoub2Karam A. Mahdy3Rehab F. Taher4Abd El-Nasser G. EI Gendy5Tarik A. Mohamed6Salim S. Al-Rejaie7Yasser A. EI-Amier8Ahmed M. Abd-EIGawad9Mohamed A. Farag10Department of Natural Compounds Chemistry, National Research Center, 33 El Bohouth St., Dokki, Giza 12622, EgyptPathology Department, National Research Centre, 33 El Bohouth St., Dokki, Giza 12622, EgyptPharmacognosy Department, Faculty of Pharmacy, Ain Shams University, Cairo 11566, EgyptMedical Biochemistry Department, National Research Centre, 33 El Bohouth St., Dokki, Giza 12622, EgyptDepartment of Natural Compounds Chemistry, National Research Center, 33 El Bohouth St., Dokki, Giza 12622, EgyptMedicinal and Aromatic Plants Research Department, National Research Centre, 33 El Bohouth St., Dokki, Giza 12622, EgyptChemistry of Medicinal Plants Department, National Research Centre, 33 El-Bohouth St., Dokki, Giza 12622, EgyptDepartment of Pharmacology & Toxicology, College of Pharmacy, King Saud University, Riyadh 11451, Saudi ArabiaDepartment of Botany, Faculty of Science, Mansoura University, Mansoura 35516, EgyptDepartment of Botany, Faculty of Science, Mansoura University, Mansoura 35516, EgyptPharmacognosy Department, College of Pharmacy, Cairo University, Kasr el Aini St., Cairo P.B. 11562, Egypt<i>Cyperus</i> has been commonly used as a multi-use medicinal plant in folk medicine worldwide. The objectives of our study were to determine the different metabolites in the <i>Cyperus conglomeratus</i> Rottb. methanol extract, and to assess its in vivo gastroprotective effect in ethanol-induced gastric ulcer model in rats. Serum levels of galactin-3 and TNF-<i>α</i> were employed as biochemical markers. To pinpoint for active agents, comprehensive metabolites profiling of extract via UPLC-qTOF-MS/MS was employed. A total of 77 chromatographic peaks were detected, of which 70 were annotated. The detected metabolites were categorized into phenolic acids and their derivatives, flavonoids, stilbenes, aurones, quinones, terpenes, and steroids. Rats were divided into six groups; healthy control, ulcer control, standard drug group, and 25, 50, 100 mg/kg of <i>C. conglomeratus</i> treated rats. Pre-treatment with <i>C. conglomeratus</i> alcohol extract significantly reduced galactin-3, and TNF-<i>α</i> in ethanol-induced ulcer model at 25, 50, and 100 mg/kg. Further histopathological and histochemical studies revealed moderate erosion of superficial epithelium, few infiltrated inflammatory cells, and depletion of gastric tissue glycoprotein in the ulcer group. Treatment with the extract protected the gastric epithelial cells in a dose-dependent manner. It could be concluded that <i>C. conglomeratus</i> extract provides significant gastroprotective activity in ethanol-induced gastric ulcer and ought to be included in nutraceuticals in the future for ulcer treatment.https://www.mdpi.com/1420-3049/25/18/4234<i>Cyperus conglomeratus</i>metabolite profilingUPLC-qTOF-MSgastroprotective activitybiochemical and histochemical characteristics |
| spellingShingle | Abdelsamed I. Elshamy Abdel Razik H. Farrag Iriny M. Ayoub Karam A. Mahdy Rehab F. Taher Abd El-Nasser G. EI Gendy Tarik A. Mohamed Salim S. Al-Rejaie Yasser A. EI-Amier Ahmed M. Abd-EIGawad Mohamed A. Farag UPLC-qTOF-MS Phytochemical Profile and Antiulcer Potential of <i>Cyperus conglomeratus</i> Rottb. Alcoholic Extract Molecules <i>Cyperus conglomeratus</i> metabolite profiling UPLC-qTOF-MS gastroprotective activity biochemical and histochemical characteristics |
| title | UPLC-qTOF-MS Phytochemical Profile and Antiulcer Potential of <i>Cyperus conglomeratus</i> Rottb. Alcoholic Extract |
| title_full | UPLC-qTOF-MS Phytochemical Profile and Antiulcer Potential of <i>Cyperus conglomeratus</i> Rottb. Alcoholic Extract |
| title_fullStr | UPLC-qTOF-MS Phytochemical Profile and Antiulcer Potential of <i>Cyperus conglomeratus</i> Rottb. Alcoholic Extract |
| title_full_unstemmed | UPLC-qTOF-MS Phytochemical Profile and Antiulcer Potential of <i>Cyperus conglomeratus</i> Rottb. Alcoholic Extract |
| title_short | UPLC-qTOF-MS Phytochemical Profile and Antiulcer Potential of <i>Cyperus conglomeratus</i> Rottb. Alcoholic Extract |
| title_sort | uplc qtof ms phytochemical profile and antiulcer potential of i cyperus conglomeratus i rottb alcoholic extract |
| topic | <i>Cyperus conglomeratus</i> metabolite profiling UPLC-qTOF-MS gastroprotective activity biochemical and histochemical characteristics |
| url | https://www.mdpi.com/1420-3049/25/18/4234 |
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