Identification and fine mapping of a new gene, BPH31 conferring resistance to brown planthopper biotype 4 of India to improve rice, Oryza sativa L
Abstract Background Rice (Oryza sativa L.) is the staple food for more than 3.5 billion people, mainly in Asia. Brown planthopper (BPH) is one of the most destructive insect pests of rice that limits rice production. Host-plant resistance is one of the most efficient ways to overcome BPH damage to t...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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SpringerOpen
2017-08-01
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| Series: | Rice |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s12284-017-0178-x |
| _version_ | 1828452471618404352 |
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| author | G. D. Prahalada N. Shivakumar H. C. Lohithaswa D. K. Sidde Gowda G. Ramkumar Sung-Ryul Kim C. Ramachandra Shailaja Hittalmani Trilochan Mohapatra Kshirod K. Jena |
| author_facet | G. D. Prahalada N. Shivakumar H. C. Lohithaswa D. K. Sidde Gowda G. Ramkumar Sung-Ryul Kim C. Ramachandra Shailaja Hittalmani Trilochan Mohapatra Kshirod K. Jena |
| author_sort | G. D. Prahalada |
| collection | DOAJ |
| description | Abstract Background Rice (Oryza sativa L.) is the staple food for more than 3.5 billion people, mainly in Asia. Brown planthopper (BPH) is one of the most destructive insect pests of rice that limits rice production. Host-plant resistance is one of the most efficient ways to overcome BPH damage to the rice crop. Results BPH bioassay studies from 2009 to 2015 conducted in India and at the International Rice Research Institute (IRRI), Philippines, revealed that the cultivar CR2711–76 developed at the National Rice Research Institute (NRRI), Cuttack, India, showed stable and broad-spectrum resistance to several BPH populations of the Philippines and BPH biotype 4 of India. Genetic analysis and fine mapping confirmed the presence of a single dominant gene, BPH31, in CR2711–76 conferring BPH resistance. The BPH31 gene was located on the long arm of chromosome 3 within an interval of 475 kb between the markers PA26 and RM2334. Bioassay analysis of the BPH31 gene in CR2711–76 was carried out against BPH populations of the Philippines. The results from bioassay revealed that CR2711–76 possesses three different mechanisms of resistance: antibiosis, antixenosis, and tolerance. The effectiveness of flanking markers was tested in a segregating population and the InDel type markers PA26 and RM2334 showed high co-segregation with the resistance phenotype. Foreground and background analysis by tightly linked markers as well as using the Infinium 6 K SNP chip respectively were applied for transferring the BPH31 gene into an indica variety, Jaya. The improved BPH31-derived Jaya lines showed strong resistance to BPH biotypes of India and the Philippines. Conclusion The new BPH31 gene can be used in BPH resistance breeding programs on the Indian subcontinent. The tightly linked DNA markers identified in the study have proved their effectiveness and can be utilized in BPH resistance breeding in rice. |
| first_indexed | 2024-12-10T23:53:23Z |
| format | Article |
| id | doaj.art-baf35fa2e3714cd1bd60089767dcb5c8 |
| institution | Directory Open Access Journal |
| issn | 1939-8425 1939-8433 |
| language | English |
| last_indexed | 2024-12-10T23:53:23Z |
| publishDate | 2017-08-01 |
| publisher | SpringerOpen |
| record_format | Article |
| series | Rice |
| spelling | doaj.art-baf35fa2e3714cd1bd60089767dcb5c82022-12-22T01:28:41ZengSpringerOpenRice1939-84251939-84332017-08-0110111510.1186/s12284-017-0178-xIdentification and fine mapping of a new gene, BPH31 conferring resistance to brown planthopper biotype 4 of India to improve rice, Oryza sativa LG. D. Prahalada0N. Shivakumar1H. C. Lohithaswa2D. K. Sidde Gowda3G. Ramkumar4Sung-Ryul Kim5C. Ramachandra6Shailaja Hittalmani7Trilochan Mohapatra8Kshirod K. Jena9Plant Breeding Division, International Rice Research InstituteZonal Agricultural Research Station, VC FarmCollege of AgricultureCollege of AgriculturePlant Breeding Division, International Rice Research InstitutePlant Breeding Division, International Rice Research InstituteZonal Agricultural Research Station, VC FarmUniversity of Agricultural Sciences, GKVKIndian Council of Agricultural ResearchPlant Breeding Division, International Rice Research InstituteAbstract Background Rice (Oryza sativa L.) is the staple food for more than 3.5 billion people, mainly in Asia. Brown planthopper (BPH) is one of the most destructive insect pests of rice that limits rice production. Host-plant resistance is one of the most efficient ways to overcome BPH damage to the rice crop. Results BPH bioassay studies from 2009 to 2015 conducted in India and at the International Rice Research Institute (IRRI), Philippines, revealed that the cultivar CR2711–76 developed at the National Rice Research Institute (NRRI), Cuttack, India, showed stable and broad-spectrum resistance to several BPH populations of the Philippines and BPH biotype 4 of India. Genetic analysis and fine mapping confirmed the presence of a single dominant gene, BPH31, in CR2711–76 conferring BPH resistance. The BPH31 gene was located on the long arm of chromosome 3 within an interval of 475 kb between the markers PA26 and RM2334. Bioassay analysis of the BPH31 gene in CR2711–76 was carried out against BPH populations of the Philippines. The results from bioassay revealed that CR2711–76 possesses three different mechanisms of resistance: antibiosis, antixenosis, and tolerance. The effectiveness of flanking markers was tested in a segregating population and the InDel type markers PA26 and RM2334 showed high co-segregation with the resistance phenotype. Foreground and background analysis by tightly linked markers as well as using the Infinium 6 K SNP chip respectively were applied for transferring the BPH31 gene into an indica variety, Jaya. The improved BPH31-derived Jaya lines showed strong resistance to BPH biotypes of India and the Philippines. Conclusion The new BPH31 gene can be used in BPH resistance breeding programs on the Indian subcontinent. The tightly linked DNA markers identified in the study have proved their effectiveness and can be utilized in BPH resistance breeding in rice.http://link.springer.com/article/10.1186/s12284-017-0178-xHost-plant resistanceBPH31InDel markerMarker-assisted selectionResistance mechanism |
| spellingShingle | G. D. Prahalada N. Shivakumar H. C. Lohithaswa D. K. Sidde Gowda G. Ramkumar Sung-Ryul Kim C. Ramachandra Shailaja Hittalmani Trilochan Mohapatra Kshirod K. Jena Identification and fine mapping of a new gene, BPH31 conferring resistance to brown planthopper biotype 4 of India to improve rice, Oryza sativa L Rice Host-plant resistance BPH31 InDel marker Marker-assisted selection Resistance mechanism |
| title | Identification and fine mapping of a new gene, BPH31 conferring resistance to brown planthopper biotype 4 of India to improve rice, Oryza sativa L |
| title_full | Identification and fine mapping of a new gene, BPH31 conferring resistance to brown planthopper biotype 4 of India to improve rice, Oryza sativa L |
| title_fullStr | Identification and fine mapping of a new gene, BPH31 conferring resistance to brown planthopper biotype 4 of India to improve rice, Oryza sativa L |
| title_full_unstemmed | Identification and fine mapping of a new gene, BPH31 conferring resistance to brown planthopper biotype 4 of India to improve rice, Oryza sativa L |
| title_short | Identification and fine mapping of a new gene, BPH31 conferring resistance to brown planthopper biotype 4 of India to improve rice, Oryza sativa L |
| title_sort | identification and fine mapping of a new gene bph31 conferring resistance to brown planthopper biotype 4 of india to improve rice oryza sativa l |
| topic | Host-plant resistance BPH31 InDel marker Marker-assisted selection Resistance mechanism |
| url | http://link.springer.com/article/10.1186/s12284-017-0178-x |
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