Purification and Characterisation of Extracellular Cellulase Main Components from Aspergillus terreus
The filamentous fungus Aspergillus terreus was cultivated in a 2-L stirred tank bioreactor, and the resulting culture filtrate was used for protein purification. From the cultivation broth, seven crude extracts of glucanase and one of β-glucosidase were purified. A total of eight components were ide...
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| Format: | Article |
| Language: | English |
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North Carolina State University
2015-06-01
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| Series: | BioResources |
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| Online Access: | http://ojs.cnr.ncsu.edu/index.php/BioRes/article/view/BioRes_10_3_4886_Shahriarinour_Purification_Extracellular_Cellulase |
| _version_ | 1811323716778328064 |
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| author | Mahdi Shahriarinour Ramakrishnan Nagasundara Ramanan |
| author_facet | Mahdi Shahriarinour Ramakrishnan Nagasundara Ramanan |
| author_sort | Mahdi Shahriarinour |
| collection | DOAJ |
| description | The filamentous fungus Aspergillus terreus was cultivated in a 2-L stirred tank bioreactor, and the resulting culture filtrate was used for protein purification. From the cultivation broth, seven crude extracts of glucanase and one of β-glucosidase were purified. A total of eight components were identified, including endoglucanases (Endo I, II, III, and IV), cellobiohydrolases (CBH I, II, and III), and β-glucosidase. The eight major components in the fermentation broth of A. terreus, which most likely constitute the essential enzymes for cellulose hydrolysis, were further purified by a series of column chromatography steps. Interestingly, the β-glucosidase from A. terreus displayed an extremely high activity on p-nitrophenyl-β-D-glucopyranoside (pNPG), which suggests that it is a good candidate enzyme for the conversion of cellobiose to glucose. The temperature and pH ranges for optimal activity of the purified enzyme were 46 to 62 °C and 5.0 to 6.0, respectively. |
| first_indexed | 2024-04-13T14:00:59Z |
| format | Article |
| id | doaj.art-bafd3274ef1045ea8dc6f2d0db4a504b |
| institution | Directory Open Access Journal |
| issn | 1930-2126 1930-2126 |
| language | English |
| last_indexed | 2024-04-13T14:00:59Z |
| publishDate | 2015-06-01 |
| publisher | North Carolina State University |
| record_format | Article |
| series | BioResources |
| spelling | doaj.art-bafd3274ef1045ea8dc6f2d0db4a504b2022-12-22T02:44:03ZengNorth Carolina State UniversityBioResources1930-21261930-21262015-06-011034886490210.15376/biores.10.3.4886-4902Purification and Characterisation of Extracellular Cellulase Main Components from Aspergillus terreusMahdi Shahriarinour0Ramakrishnan Nagasundara Ramanan1Department of Microbiology, Science and Research Branch, Islamic Azad University, Guilan, Iran; Iran, Islamic Republic OfChemical and Sustainable Process Engineering Research Group, School of Engineering, Monash University, Bandar Sunway 46150, Malaysia; MalaysiaThe filamentous fungus Aspergillus terreus was cultivated in a 2-L stirred tank bioreactor, and the resulting culture filtrate was used for protein purification. From the cultivation broth, seven crude extracts of glucanase and one of β-glucosidase were purified. A total of eight components were identified, including endoglucanases (Endo I, II, III, and IV), cellobiohydrolases (CBH I, II, and III), and β-glucosidase. The eight major components in the fermentation broth of A. terreus, which most likely constitute the essential enzymes for cellulose hydrolysis, were further purified by a series of column chromatography steps. Interestingly, the β-glucosidase from A. terreus displayed an extremely high activity on p-nitrophenyl-β-D-glucopyranoside (pNPG), which suggests that it is a good candidate enzyme for the conversion of cellobiose to glucose. The temperature and pH ranges for optimal activity of the purified enzyme were 46 to 62 °C and 5.0 to 6.0, respectively.http://ojs.cnr.ncsu.edu/index.php/BioRes/article/view/BioRes_10_3_4886_Shahriarinour_Purification_Extracellular_CellulaseCellulase enzymesPurificationAspergillus terreusChromatography |
| spellingShingle | Mahdi Shahriarinour Ramakrishnan Nagasundara Ramanan Purification and Characterisation of Extracellular Cellulase Main Components from Aspergillus terreus BioResources Cellulase enzymes Purification Aspergillus terreus Chromatography |
| title | Purification and Characterisation of Extracellular Cellulase Main Components from Aspergillus terreus |
| title_full | Purification and Characterisation of Extracellular Cellulase Main Components from Aspergillus terreus |
| title_fullStr | Purification and Characterisation of Extracellular Cellulase Main Components from Aspergillus terreus |
| title_full_unstemmed | Purification and Characterisation of Extracellular Cellulase Main Components from Aspergillus terreus |
| title_short | Purification and Characterisation of Extracellular Cellulase Main Components from Aspergillus terreus |
| title_sort | purification and characterisation of extracellular cellulase main components from aspergillus terreus |
| topic | Cellulase enzymes Purification Aspergillus terreus Chromatography |
| url | http://ojs.cnr.ncsu.edu/index.php/BioRes/article/view/BioRes_10_3_4886_Shahriarinour_Purification_Extracellular_Cellulase |
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