Comparison of pathogenic bacteria in the upper and lower respiratory tracts of cattle either directly transported to a feedlot or co-mingled at auction markets prior to feedlot placement
IntroductionBacterial bronchopneumonia (BP) has been associated with purchasing cattle through auction markets. However, whether auction markets are a source of BP-associated bacterial pathogens is unknown. This study evaluated prevalence, antimicrobial susceptibility, and genetic relatedness (using...
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Frontiers Media S.A.
2023-01-01
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| Series: | Frontiers in Veterinary Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fvets.2022.1026470/full |
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| author | Christina Hirsch Edouard Timsit Muhammed Salah Uddin Muhammed Salah Uddin Le Luo Guan Trevor W. Alexander |
| author_facet | Christina Hirsch Edouard Timsit Muhammed Salah Uddin Muhammed Salah Uddin Le Luo Guan Trevor W. Alexander |
| author_sort | Christina Hirsch |
| collection | DOAJ |
| description | IntroductionBacterial bronchopneumonia (BP) has been associated with purchasing cattle through auction markets. However, whether auction markets are a source of BP-associated bacterial pathogens is unknown. This study evaluated prevalence, antimicrobial susceptibility, and genetic relatedness (using pulsed-field gel electrophoresis, PFGE) of Mannheimia haemolytica, Pasteurella multocida, and Histophilus somni isolated from cattle either transported to an auction market prior to feedlot placement (AUC), or directly to a feedlot from a farm (RANC).MethodsTwo groups of cattle were enrolled (N = 30 per group) from two separate farms with 15 animals from an individual farm designated as AUC or RANC. Deep nasal swab (DNS) and trans-tracheal aspirates (TTA) were collected on day 0 at weaning (T0) and on day 2 at on-arrival processing at the feedlot (T1). The DNS were also collected on day 9 (T2) and day 30 (T3) after arrival at the feedlot.Results and discussionIn both TTA and DNS, prevalence of bacteria did not differ between AUC and RANC groups (P > 0.05). None of the bacteria isolated at T0 were resistant to antimicrobials and diversity of all bacteria was greatest at T0 and T1. In Group 1 cattle, 100% of P. multocida isolated at T2 and T3 were multi-drug resistant. These isolates were highly related (>90%) according to PFGE, with most being clones. Though limited in size, results for animals evaluated in this study suggested that auction markets were not a major source of resistant BP pathogens, however, horizontal transmission of a multi-resistant strain of P. multocida occurred in a feedlot. Spread of resistant P. multocida was likely due to the selective pressures imposed by feedlot antimicrobial use and encoded resistance by the bacteria. |
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| issn | 2297-1769 |
| language | English |
| last_indexed | 2024-04-10T20:47:17Z |
| publishDate | 2023-01-01 |
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| series | Frontiers in Veterinary Science |
| spelling | doaj.art-bb01686d39014a02a7c03d762651609f2023-01-24T07:07:53ZengFrontiers Media S.A.Frontiers in Veterinary Science2297-17692023-01-01910.3389/fvets.2022.10264701026470Comparison of pathogenic bacteria in the upper and lower respiratory tracts of cattle either directly transported to a feedlot or co-mingled at auction markets prior to feedlot placementChristina Hirsch0Edouard Timsit1Muhammed Salah Uddin2Muhammed Salah Uddin3Le Luo Guan4Trevor W. Alexander5Ceva Santé Animale, Libourne, FranceCeva Santé Animale, Libourne, FranceLethbridge Research and Development Centre, Agriculture and Agri-Food Canada, Lethbridge, AB, CanadaDepartment of Agricultural, Food and Nutritional Science, University of Alberta, Edmonton, AB, CanadaDepartment of Agricultural, Food and Nutritional Science, University of Alberta, Edmonton, AB, CanadaLethbridge Research and Development Centre, Agriculture and Agri-Food Canada, Lethbridge, AB, CanadaIntroductionBacterial bronchopneumonia (BP) has been associated with purchasing cattle through auction markets. However, whether auction markets are a source of BP-associated bacterial pathogens is unknown. This study evaluated prevalence, antimicrobial susceptibility, and genetic relatedness (using pulsed-field gel electrophoresis, PFGE) of Mannheimia haemolytica, Pasteurella multocida, and Histophilus somni isolated from cattle either transported to an auction market prior to feedlot placement (AUC), or directly to a feedlot from a farm (RANC).MethodsTwo groups of cattle were enrolled (N = 30 per group) from two separate farms with 15 animals from an individual farm designated as AUC or RANC. Deep nasal swab (DNS) and trans-tracheal aspirates (TTA) were collected on day 0 at weaning (T0) and on day 2 at on-arrival processing at the feedlot (T1). The DNS were also collected on day 9 (T2) and day 30 (T3) after arrival at the feedlot.Results and discussionIn both TTA and DNS, prevalence of bacteria did not differ between AUC and RANC groups (P > 0.05). None of the bacteria isolated at T0 were resistant to antimicrobials and diversity of all bacteria was greatest at T0 and T1. In Group 1 cattle, 100% of P. multocida isolated at T2 and T3 were multi-drug resistant. These isolates were highly related (>90%) according to PFGE, with most being clones. Though limited in size, results for animals evaluated in this study suggested that auction markets were not a major source of resistant BP pathogens, however, horizontal transmission of a multi-resistant strain of P. multocida occurred in a feedlot. Spread of resistant P. multocida was likely due to the selective pressures imposed by feedlot antimicrobial use and encoded resistance by the bacteria.https://www.frontiersin.org/articles/10.3389/fvets.2022.1026470/fullantimicrobial resistanceauction marketbacterial bronchopneumoniabovine respiratory diseasecattlefeedlot |
| spellingShingle | Christina Hirsch Edouard Timsit Muhammed Salah Uddin Muhammed Salah Uddin Le Luo Guan Trevor W. Alexander Comparison of pathogenic bacteria in the upper and lower respiratory tracts of cattle either directly transported to a feedlot or co-mingled at auction markets prior to feedlot placement Frontiers in Veterinary Science antimicrobial resistance auction market bacterial bronchopneumonia bovine respiratory disease cattle feedlot |
| title | Comparison of pathogenic bacteria in the upper and lower respiratory tracts of cattle either directly transported to a feedlot or co-mingled at auction markets prior to feedlot placement |
| title_full | Comparison of pathogenic bacteria in the upper and lower respiratory tracts of cattle either directly transported to a feedlot or co-mingled at auction markets prior to feedlot placement |
| title_fullStr | Comparison of pathogenic bacteria in the upper and lower respiratory tracts of cattle either directly transported to a feedlot or co-mingled at auction markets prior to feedlot placement |
| title_full_unstemmed | Comparison of pathogenic bacteria in the upper and lower respiratory tracts of cattle either directly transported to a feedlot or co-mingled at auction markets prior to feedlot placement |
| title_short | Comparison of pathogenic bacteria in the upper and lower respiratory tracts of cattle either directly transported to a feedlot or co-mingled at auction markets prior to feedlot placement |
| title_sort | comparison of pathogenic bacteria in the upper and lower respiratory tracts of cattle either directly transported to a feedlot or co mingled at auction markets prior to feedlot placement |
| topic | antimicrobial resistance auction market bacterial bronchopneumonia bovine respiratory disease cattle feedlot |
| url | https://www.frontiersin.org/articles/10.3389/fvets.2022.1026470/full |
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