Correlations Between the Collagen Content of the Human Left Ventricular Myocardium, Measured by Biochemical and Morphometric Methods
OBJECTIVE: To assess, in myocardium specimens obtained from necropsies, the correlation between the concentration of hydroxyproline, measured with the photocolorimetric method, and the intensity of fibrosis, determined with the morphometric method. METHODS: Left ventricle myocardium samples were obt...
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| Format: | Article |
| Language: | English |
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Sociedade Brasileira de Cardiologia (SBC)
2002-07-01
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| Series: | Arquivos Brasileiros de Cardiologia |
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| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0066-782X2002001000002 |
| _version_ | 1819266859086643200 |
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| author | José Duarte de Morais Lopes Roseli Aparecida da Silva Gomes Valdemar Hial Isabel Cristina Rezende Lopes Marlene Antônia dos Reis Vicente de Paula Antunes Teixeira |
| author_facet | José Duarte de Morais Lopes Roseli Aparecida da Silva Gomes Valdemar Hial Isabel Cristina Rezende Lopes Marlene Antônia dos Reis Vicente de Paula Antunes Teixeira |
| author_sort | José Duarte de Morais Lopes |
| collection | DOAJ |
| description | OBJECTIVE: To assess, in myocardium specimens obtained from necropsies, the correlation between the concentration of hydroxyproline, measured with the photocolorimetric method, and the intensity of fibrosis, determined with the morphometric method. METHODS: Left ventricle myocardium samples were obtained from 45 patients who had undergone necropsy, some of them with a variety of cardiopathies and others without any heart disease. The concentrations of hydroxyproline were determined with the photocolorimetric method. In the histologic sections from each heart, the myocardial fibrosis was quantified by using a light microscope with an integrating ocular lens. RESULTS: A median of, respectively, 4.5 and 4.3 mug of hydroxyproline/mg of dry weight was found in fixed and nonfixed left ventricle myocardium fragments. A positive correlation occurred between the hydroxyproline concentrations and the intensity of fibrosis, both in the fixed (Sr=+0.25; p=0.099) and in the nonfixed (Sr=+0.32; p=0.03) specimens. CONCLUSION: The biochemical methodology was proven to be adequate, and manual morphometry was shown to have limitations that may interfere with the statistical significance of correlations for the estimate of fibrosis intensity in the human myocardium. |
| first_indexed | 2024-12-23T21:07:58Z |
| format | Article |
| id | doaj.art-bb0452f975a64ea6bb72eba6c2b61b9d |
| institution | Directory Open Access Journal |
| issn | 0066-782X 1678-4170 |
| language | English |
| last_indexed | 2024-12-23T21:07:58Z |
| publishDate | 2002-07-01 |
| publisher | Sociedade Brasileira de Cardiologia (SBC) |
| record_format | Article |
| series | Arquivos Brasileiros de Cardiologia |
| spelling | doaj.art-bb0452f975a64ea6bb72eba6c2b61b9d2022-12-21T17:31:09ZengSociedade Brasileira de Cardiologia (SBC)Arquivos Brasileiros de Cardiologia0066-782X1678-41702002-07-01791151910.1590/S0066-782X2002001000002Correlations Between the Collagen Content of the Human Left Ventricular Myocardium, Measured by Biochemical and Morphometric MethodsJosé Duarte de Morais LopesRoseli Aparecida da Silva GomesValdemar HialIsabel Cristina Rezende LopesMarlene Antônia dos ReisVicente de Paula Antunes TeixeiraOBJECTIVE: To assess, in myocardium specimens obtained from necropsies, the correlation between the concentration of hydroxyproline, measured with the photocolorimetric method, and the intensity of fibrosis, determined with the morphometric method. METHODS: Left ventricle myocardium samples were obtained from 45 patients who had undergone necropsy, some of them with a variety of cardiopathies and others without any heart disease. The concentrations of hydroxyproline were determined with the photocolorimetric method. In the histologic sections from each heart, the myocardial fibrosis was quantified by using a light microscope with an integrating ocular lens. RESULTS: A median of, respectively, 4.5 and 4.3 mug of hydroxyproline/mg of dry weight was found in fixed and nonfixed left ventricle myocardium fragments. A positive correlation occurred between the hydroxyproline concentrations and the intensity of fibrosis, both in the fixed (Sr=+0.25; p=0.099) and in the nonfixed (Sr=+0.32; p=0.03) specimens. CONCLUSION: The biochemical methodology was proven to be adequate, and manual morphometry was shown to have limitations that may interfere with the statistical significance of correlations for the estimate of fibrosis intensity in the human myocardium.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0066-782X2002001000002hearthydroxyprolinemorphometry |
| spellingShingle | José Duarte de Morais Lopes Roseli Aparecida da Silva Gomes Valdemar Hial Isabel Cristina Rezende Lopes Marlene Antônia dos Reis Vicente de Paula Antunes Teixeira Correlations Between the Collagen Content of the Human Left Ventricular Myocardium, Measured by Biochemical and Morphometric Methods Arquivos Brasileiros de Cardiologia heart hydroxyproline morphometry |
| title | Correlations Between the Collagen Content of the Human Left Ventricular Myocardium, Measured by Biochemical and Morphometric Methods |
| title_full | Correlations Between the Collagen Content of the Human Left Ventricular Myocardium, Measured by Biochemical and Morphometric Methods |
| title_fullStr | Correlations Between the Collagen Content of the Human Left Ventricular Myocardium, Measured by Biochemical and Morphometric Methods |
| title_full_unstemmed | Correlations Between the Collagen Content of the Human Left Ventricular Myocardium, Measured by Biochemical and Morphometric Methods |
| title_short | Correlations Between the Collagen Content of the Human Left Ventricular Myocardium, Measured by Biochemical and Morphometric Methods |
| title_sort | correlations between the collagen content of the human left ventricular myocardium measured by biochemical and morphometric methods |
| topic | heart hydroxyproline morphometry |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0066-782X2002001000002 |
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