Soil Bacteria as Potential Biological Control Agents of <i>Fusarium</i> Species Associated with Asparagus Decline Syndrome
To avoid the use of agrochemicals in agriculture, alternative methods are emerging to control plant pathogens. Some plant growth-promoting rhizobacteria (PGPR) can be used as biocontrol agents since they can induce protection against pathogens. The aim of this study was to evaluate the protective ef...
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MDPI AG
2021-09-01
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| Series: | Applied Sciences |
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| Online Access: | https://www.mdpi.com/2076-3417/11/18/8356 |
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| author | Eduardo De la Lastra María Camacho Nieves Capote |
| author_facet | Eduardo De la Lastra María Camacho Nieves Capote |
| author_sort | Eduardo De la Lastra |
| collection | DOAJ |
| description | To avoid the use of agrochemicals in agriculture, alternative methods are emerging to control plant pathogens. Some plant growth-promoting rhizobacteria (PGPR) can be used as biocontrol agents since they can induce protection against pathogens. The aim of this study was to evaluate the protective effect of several PGPR strains against the main <i>Fusarium</i> species involved in asparagus decline syndrome (ADS): <i>F. proliferatum</i>, <i>F. oxysporum</i> f. sp. <i>asparagi</i> and <i>F. redolens</i>. <i>In vitro</i> antagonism assays showed that all the bacteria inhibited the mycelium growth of the three <i>Fusarium</i> species. The most effective strains (<i>Streptomyces fradiae</i> Hvs6, <i>Bacillus paralicheniformis</i> Hvs2 and <i>Bacillus velezensis</i> FC37) were tested to evaluate their protective effect on asparagus plants inoculated with pathogenic <i>Fusarium</i> isolates. Strains FC37 and Hvs2 were the most effective in controlling pathogenic <i>F. proliferatum</i> and <i>F. oxysporum</i> f. sp. <i>asparagi</i>, but neither could protect against <i>F. redolens</i> isolates. The production of hydrolytic enzymes such as β-glucosidase, amylase and protease by these bacterial strains could be involved in the structural degradation of the fungal cell wall. In addition, the production of toxic volatile compounds, such as hydrogen cyanide, may inhibit the fungal growth, and the production of phosphate solubilizers could be related to the plant growth promotion. These results suggest that strains FC37 and Hvs2 could be used as potential biocontrol agents as a sustainable and environmentally friendly control strategy for ADS-affected fields. |
| first_indexed | 2024-03-10T07:56:24Z |
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| language | English |
| last_indexed | 2024-03-10T07:56:24Z |
| publishDate | 2021-09-01 |
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| spelling | doaj.art-bb05055d198d49708440f7ecd81c50fb2023-11-22T11:51:27ZengMDPI AGApplied Sciences2076-34172021-09-011118835610.3390/app11188356Soil Bacteria as Potential Biological Control Agents of <i>Fusarium</i> Species Associated with Asparagus Decline SyndromeEduardo De la Lastra0María Camacho1Nieves Capote2Andalusian Institute of Agricultural and Fisheries Research and Training (IFAPA), Center “Las Torres”, 41200 Seville, SpainAndalusian Institute of Agricultural and Fisheries Research and Training (IFAPA), Center “Las Torres”, 41200 Seville, SpainAndalusian Institute of Agricultural and Fisheries Research and Training (IFAPA), Center “Las Torres”, 41200 Seville, SpainTo avoid the use of agrochemicals in agriculture, alternative methods are emerging to control plant pathogens. Some plant growth-promoting rhizobacteria (PGPR) can be used as biocontrol agents since they can induce protection against pathogens. The aim of this study was to evaluate the protective effect of several PGPR strains against the main <i>Fusarium</i> species involved in asparagus decline syndrome (ADS): <i>F. proliferatum</i>, <i>F. oxysporum</i> f. sp. <i>asparagi</i> and <i>F. redolens</i>. <i>In vitro</i> antagonism assays showed that all the bacteria inhibited the mycelium growth of the three <i>Fusarium</i> species. The most effective strains (<i>Streptomyces fradiae</i> Hvs6, <i>Bacillus paralicheniformis</i> Hvs2 and <i>Bacillus velezensis</i> FC37) were tested to evaluate their protective effect on asparagus plants inoculated with pathogenic <i>Fusarium</i> isolates. Strains FC37 and Hvs2 were the most effective in controlling pathogenic <i>F. proliferatum</i> and <i>F. oxysporum</i> f. sp. <i>asparagi</i>, but neither could protect against <i>F. redolens</i> isolates. The production of hydrolytic enzymes such as β-glucosidase, amylase and protease by these bacterial strains could be involved in the structural degradation of the fungal cell wall. In addition, the production of toxic volatile compounds, such as hydrogen cyanide, may inhibit the fungal growth, and the production of phosphate solubilizers could be related to the plant growth promotion. These results suggest that strains FC37 and Hvs2 could be used as potential biocontrol agents as a sustainable and environmentally friendly control strategy for ADS-affected fields.https://www.mdpi.com/2076-3417/11/18/8356<i>Fusarium proliferatum</i><i>F. oxysporum</i>enzymatic activity<i>Bacillus paralicheniformis</i><i>Bacillus velezensis</i>biocontrol |
| spellingShingle | Eduardo De la Lastra María Camacho Nieves Capote Soil Bacteria as Potential Biological Control Agents of <i>Fusarium</i> Species Associated with Asparagus Decline Syndrome Applied Sciences <i>Fusarium proliferatum</i> <i>F. oxysporum</i> enzymatic activity <i>Bacillus paralicheniformis</i> <i>Bacillus velezensis</i> biocontrol |
| title | Soil Bacteria as Potential Biological Control Agents of <i>Fusarium</i> Species Associated with Asparagus Decline Syndrome |
| title_full | Soil Bacteria as Potential Biological Control Agents of <i>Fusarium</i> Species Associated with Asparagus Decline Syndrome |
| title_fullStr | Soil Bacteria as Potential Biological Control Agents of <i>Fusarium</i> Species Associated with Asparagus Decline Syndrome |
| title_full_unstemmed | Soil Bacteria as Potential Biological Control Agents of <i>Fusarium</i> Species Associated with Asparagus Decline Syndrome |
| title_short | Soil Bacteria as Potential Biological Control Agents of <i>Fusarium</i> Species Associated with Asparagus Decline Syndrome |
| title_sort | soil bacteria as potential biological control agents of i fusarium i species associated with asparagus decline syndrome |
| topic | <i>Fusarium proliferatum</i> <i>F. oxysporum</i> enzymatic activity <i>Bacillus paralicheniformis</i> <i>Bacillus velezensis</i> biocontrol |
| url | https://www.mdpi.com/2076-3417/11/18/8356 |
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