Galactoligosaccharide and a prebiotic blend improve colonic health and immunity of adult dogs.
This study aimed to evaluate the effects of two prebiotics in different concentrations on nutrient digestibility, fermentative products and immunological variables in adult dogs. Twenty-four adult dogs were randomly divided into six blocks according to their metabolic body weights (BW0.75); within t...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2020-01-01
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| Series: | PLoS ONE |
| Online Access: | https://doi.org/10.1371/journal.pone.0238006 |
| _version_ | 1818985702645301248 |
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| author | Mariana Fragoso Rentas Raquel Silveira Pedreira Mariana Pamplona Perini Larissa Wünsche Risolia Rafael Vessecchi Amorim Zafalon Isabella Corsato Alvarenga Thiago Henrique Annibale Vendramini Júlio Cesar Carvalho Balieiro Cristiana Ferreira Fonseca Pontieri Marcio Antonio Brunetto |
| author_facet | Mariana Fragoso Rentas Raquel Silveira Pedreira Mariana Pamplona Perini Larissa Wünsche Risolia Rafael Vessecchi Amorim Zafalon Isabella Corsato Alvarenga Thiago Henrique Annibale Vendramini Júlio Cesar Carvalho Balieiro Cristiana Ferreira Fonseca Pontieri Marcio Antonio Brunetto |
| author_sort | Mariana Fragoso Rentas |
| collection | DOAJ |
| description | This study aimed to evaluate the effects of two prebiotics in different concentrations on nutrient digestibility, fermentative products and immunological variables in adult dogs. Twenty-four adult dogs were randomly divided into six blocks according to their metabolic body weights (BW0.75); within these groups, dogs were randomized to four treatments: control without prebiotics (CO); inclusion of 0.5% prebiotic blend Yes-Golf (B1); inclusion of 1.0% galactooligosaccharide (GOS); and inclusion of 1.0% prebiotic blend Yes-Golf (B2). The experiment lasted 30 days, with 20 days adaptation and 10 days stool and blood collection. Results were analyzed for normality and means were separated by ANOVA and adjusted by the Tukey test at the significance level of 5.0%. Prebiotic supplementation had no effect on apparent digestibility coefficients (ADC), total stool production and fecal scores (p > 0.05). Prebiotics evaluated also did not alter fecal pH, nor the concentrations of ammonia, lactic acid, short chain fatty acids (SCFA) and most fecal branched chain fatty acids (BCFA) (p > 0.05). The addition of GOS decreased the concentration of iso-valeric acid (p = 0.0423). Regarding immunological variables, concentrations of fecal IgA were not influenced by the treatments. Treatments GOS and B2 increased the total number of polymorphonuclear cells, as well as the oxidative burst in relation to treatments B1 and CO (p < 0.0001). Treatment B2 improved the rate of S. aureus phagocytosis in relation to CO (p = 0.0111), and both the GOS and B2 treatments had a better index for E. coli phagocytosis than the CO treatment (p = 0.0067). In conclusion, there was indication that both prebiotics GOS and B2 at 1.0% inclusion improved the immunity of healthy dogs. |
| first_indexed | 2024-12-20T18:39:06Z |
| format | Article |
| id | doaj.art-bb0641612ed9490ba32dd4b2dc2b3a05 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-20T18:39:06Z |
| publishDate | 2020-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-bb0641612ed9490ba32dd4b2dc2b3a052022-12-21T19:29:51ZengPublic Library of Science (PLoS)PLoS ONE1932-62032020-01-01158e023800610.1371/journal.pone.0238006Galactoligosaccharide and a prebiotic blend improve colonic health and immunity of adult dogs.Mariana Fragoso RentasRaquel Silveira PedreiraMariana Pamplona PeriniLarissa Wünsche RisoliaRafael Vessecchi Amorim ZafalonIsabella Corsato AlvarengaThiago Henrique Annibale VendraminiJúlio Cesar Carvalho BalieiroCristiana Ferreira Fonseca PontieriMarcio Antonio BrunettoThis study aimed to evaluate the effects of two prebiotics in different concentrations on nutrient digestibility, fermentative products and immunological variables in adult dogs. Twenty-four adult dogs were randomly divided into six blocks according to their metabolic body weights (BW0.75); within these groups, dogs were randomized to four treatments: control without prebiotics (CO); inclusion of 0.5% prebiotic blend Yes-Golf (B1); inclusion of 1.0% galactooligosaccharide (GOS); and inclusion of 1.0% prebiotic blend Yes-Golf (B2). The experiment lasted 30 days, with 20 days adaptation and 10 days stool and blood collection. Results were analyzed for normality and means were separated by ANOVA and adjusted by the Tukey test at the significance level of 5.0%. Prebiotic supplementation had no effect on apparent digestibility coefficients (ADC), total stool production and fecal scores (p > 0.05). Prebiotics evaluated also did not alter fecal pH, nor the concentrations of ammonia, lactic acid, short chain fatty acids (SCFA) and most fecal branched chain fatty acids (BCFA) (p > 0.05). The addition of GOS decreased the concentration of iso-valeric acid (p = 0.0423). Regarding immunological variables, concentrations of fecal IgA were not influenced by the treatments. Treatments GOS and B2 increased the total number of polymorphonuclear cells, as well as the oxidative burst in relation to treatments B1 and CO (p < 0.0001). Treatment B2 improved the rate of S. aureus phagocytosis in relation to CO (p = 0.0111), and both the GOS and B2 treatments had a better index for E. coli phagocytosis than the CO treatment (p = 0.0067). In conclusion, there was indication that both prebiotics GOS and B2 at 1.0% inclusion improved the immunity of healthy dogs.https://doi.org/10.1371/journal.pone.0238006 |
| spellingShingle | Mariana Fragoso Rentas Raquel Silveira Pedreira Mariana Pamplona Perini Larissa Wünsche Risolia Rafael Vessecchi Amorim Zafalon Isabella Corsato Alvarenga Thiago Henrique Annibale Vendramini Júlio Cesar Carvalho Balieiro Cristiana Ferreira Fonseca Pontieri Marcio Antonio Brunetto Galactoligosaccharide and a prebiotic blend improve colonic health and immunity of adult dogs. PLoS ONE |
| title | Galactoligosaccharide and a prebiotic blend improve colonic health and immunity of adult dogs. |
| title_full | Galactoligosaccharide and a prebiotic blend improve colonic health and immunity of adult dogs. |
| title_fullStr | Galactoligosaccharide and a prebiotic blend improve colonic health and immunity of adult dogs. |
| title_full_unstemmed | Galactoligosaccharide and a prebiotic blend improve colonic health and immunity of adult dogs. |
| title_short | Galactoligosaccharide and a prebiotic blend improve colonic health and immunity of adult dogs. |
| title_sort | galactoligosaccharide and a prebiotic blend improve colonic health and immunity of adult dogs |
| url | https://doi.org/10.1371/journal.pone.0238006 |
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