Silver Oxide Coatings with High Silver-Ion Elution Rates and Characterization of Bactericidal Activity
This paper reports the synthesis and characterization of silver oxide films for use as bactericidal coatings. Synthesis parameters, dissolution/elution rate, and bactericidal efficacy are reported. Synthesis conditions were developed to create AgO, Ag2O, or mixtures of AgO and Ag2O on surfaces by re...
| Main Authors: | , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2017-09-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/22/9/1487 |
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| author | Sarah S. Goderecci Eric Kaiser Michael Yanakas Zachary Norris Jeffrey Scaturro Robert Oszust Clarence D. Medina Fallon Waechter Min Heon Robert R. Krchnavek Lei Yu Samuel E. Lofland Renee M. Demarest Gregory A. Caputo Jeffrey D. Hettinger |
| author_facet | Sarah S. Goderecci Eric Kaiser Michael Yanakas Zachary Norris Jeffrey Scaturro Robert Oszust Clarence D. Medina Fallon Waechter Min Heon Robert R. Krchnavek Lei Yu Samuel E. Lofland Renee M. Demarest Gregory A. Caputo Jeffrey D. Hettinger |
| author_sort | Sarah S. Goderecci |
| collection | DOAJ |
| description | This paper reports the synthesis and characterization of silver oxide films for use as bactericidal coatings. Synthesis parameters, dissolution/elution rate, and bactericidal efficacy are reported. Synthesis conditions were developed to create AgO, Ag2O, or mixtures of AgO and Ag2O on surfaces by reactive magnetron sputtering. The coatings demonstrate strong adhesion to many substrate materials and impede the growth of all bacterial strains tested. The coatings are effective in killing Escherichia coli and Staphylococcus aureus, demonstrating a clear zone-of-inhibition against bacteria growing on solid media and the ability to rapidly inhibit bacterial growth in planktonic culture. Additionally, the coatings exhibit very high elution of silver ions under conditions that mimic dynamic fluid flow ranging between 0.003 and 0.07 ppm/min depending on the media conditions. The elution of silver ions from the AgO/Ag2O surfaces was directly impacted by the complexity of the elution media, with a reduction in elution rate when examined in complex cell culture media. Both E. coli and S. aureus were shown to bind ~1 ppm Ag+/mL culture. The elution of Ag+ resulted in no increases in mammalian cell apoptosis after 24 h exposure compared to control, but apoptotic cells increased to ~35% by 48 and 72 h of exposure. Taken together, the AgO/Ag2O coatings described are effective in eliciting antibacterial activity and have potential for application on a wide variety of surfaces and devices. |
| first_indexed | 2024-04-12T11:51:51Z |
| format | Article |
| id | doaj.art-bb075bc928224373a22dcb8a5658345d |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-04-12T11:51:51Z |
| publishDate | 2017-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-bb075bc928224373a22dcb8a5658345d2022-12-22T03:34:10ZengMDPI AGMolecules1420-30492017-09-01229148710.3390/molecules22091487molecules22091487Silver Oxide Coatings with High Silver-Ion Elution Rates and Characterization of Bactericidal ActivitySarah S. Goderecci0Eric Kaiser1Michael Yanakas2Zachary Norris3Jeffrey Scaturro4Robert Oszust5Clarence D. Medina6Fallon Waechter7Min Heon8Robert R. Krchnavek9Lei Yu10Samuel E. Lofland11Renee M. Demarest12Gregory A. Caputo13Jeffrey D. Hettinger14Department of Chemistry and Biochemistry, Rowan University, Glassboro, NJ 08028, USADepartment of Physics and Astronomy, Rowan University, Glassboro, NJ 08028, USADepartment of Physics and Astronomy, Rowan University, Glassboro, NJ 08028, USADepartment of Physics and Astronomy, Rowan University, Glassboro, NJ 08028, USADepartment of Physics and Astronomy, Rowan University, Glassboro, NJ 08028, USADepartment of Physics and Astronomy, Rowan University, Glassboro, NJ 08028, USADepartment of Chemistry and Biochemistry, Rowan University, Glassboro, NJ 08028, USADepartment of Chemistry and Biochemistry, Rowan University, Glassboro, NJ 08028, USADepartment of Materials Science and Engineering, Drexel University, Philadelphia, PA 19104, USADepartment of Electrical and Computer Engineering, Rowan University, Glassboro, NJ 08028, USADepartment of Chemistry and Biochemistry, Rowan University, Glassboro, NJ 08028, USADepartment of Physics and Astronomy, Rowan University, Glassboro, NJ 08028, USADepartment of Molecular Biology, Rowan University, Stratford, NJ 08084, USADepartment of Chemistry and Biochemistry, Rowan University, Glassboro, NJ 08028, USADepartment of Physics and Astronomy, Rowan University, Glassboro, NJ 08028, USAThis paper reports the synthesis and characterization of silver oxide films for use as bactericidal coatings. Synthesis parameters, dissolution/elution rate, and bactericidal efficacy are reported. Synthesis conditions were developed to create AgO, Ag2O, or mixtures of AgO and Ag2O on surfaces by reactive magnetron sputtering. The coatings demonstrate strong adhesion to many substrate materials and impede the growth of all bacterial strains tested. The coatings are effective in killing Escherichia coli and Staphylococcus aureus, demonstrating a clear zone-of-inhibition against bacteria growing on solid media and the ability to rapidly inhibit bacterial growth in planktonic culture. Additionally, the coatings exhibit very high elution of silver ions under conditions that mimic dynamic fluid flow ranging between 0.003 and 0.07 ppm/min depending on the media conditions. The elution of silver ions from the AgO/Ag2O surfaces was directly impacted by the complexity of the elution media, with a reduction in elution rate when examined in complex cell culture media. Both E. coli and S. aureus were shown to bind ~1 ppm Ag+/mL culture. The elution of Ag+ resulted in no increases in mammalian cell apoptosis after 24 h exposure compared to control, but apoptotic cells increased to ~35% by 48 and 72 h of exposure. Taken together, the AgO/Ag2O coatings described are effective in eliciting antibacterial activity and have potential for application on a wide variety of surfaces and devices.https://www.mdpi.com/1420-3049/22/9/1487bactericidal coatingsantibacterialsilver oxidereactive sputteringthin film coatings |
| spellingShingle | Sarah S. Goderecci Eric Kaiser Michael Yanakas Zachary Norris Jeffrey Scaturro Robert Oszust Clarence D. Medina Fallon Waechter Min Heon Robert R. Krchnavek Lei Yu Samuel E. Lofland Renee M. Demarest Gregory A. Caputo Jeffrey D. Hettinger Silver Oxide Coatings with High Silver-Ion Elution Rates and Characterization of Bactericidal Activity Molecules bactericidal coatings antibacterial silver oxide reactive sputtering thin film coatings |
| title | Silver Oxide Coatings with High Silver-Ion Elution Rates and Characterization of Bactericidal Activity |
| title_full | Silver Oxide Coatings with High Silver-Ion Elution Rates and Characterization of Bactericidal Activity |
| title_fullStr | Silver Oxide Coatings with High Silver-Ion Elution Rates and Characterization of Bactericidal Activity |
| title_full_unstemmed | Silver Oxide Coatings with High Silver-Ion Elution Rates and Characterization of Bactericidal Activity |
| title_short | Silver Oxide Coatings with High Silver-Ion Elution Rates and Characterization of Bactericidal Activity |
| title_sort | silver oxide coatings with high silver ion elution rates and characterization of bactericidal activity |
| topic | bactericidal coatings antibacterial silver oxide reactive sputtering thin film coatings |
| url | https://www.mdpi.com/1420-3049/22/9/1487 |
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