Initial nutrient condition determines the recovery speed of quiescent cells in fission yeast
Most of microbe cells spend the majority of their times in quiescence due to unfavorable environmental conditions. The study of this dominant state is crucial for understanding the basic cell physiology. Retained recovery ability is a critical property of quiescent cells, which consists of two featu...
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| Format: | Article |
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Elsevier
2024-03-01
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| Series: | Heliyon |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2405844024025891 |
| _version_ | 1797259885931397120 |
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| author | Qi Liu Nan Sheng Zhiwen Zhang Chenjun He Yao Zhao Haoyuan Sun Jianguo Chen Xiaojing Yang Chao Tang |
| author_facet | Qi Liu Nan Sheng Zhiwen Zhang Chenjun He Yao Zhao Haoyuan Sun Jianguo Chen Xiaojing Yang Chao Tang |
| author_sort | Qi Liu |
| collection | DOAJ |
| description | Most of microbe cells spend the majority of their times in quiescence due to unfavorable environmental conditions. The study of this dominant state is crucial for understanding the basic cell physiology. Retained recovery ability is a critical property of quiescent cells, which consists of two features: how long the cells can survive (the survivability) and how fast they can recover (the recovery activity). While the survivability has been extensively studied under the background of chronological aging, how the recovery activity depends on the quiescent time and what factors influence its dynamics have not been addressed quantitatively. In this work, we systematically quantified both the survivability and the recovery activity of long-lived quiescent fission yeast cells at the single cell level under various nutrient conditions. It provides the most profound evolutionary dynamics of quiescent cell regeneration ability described to date. We found that the single cell recovery time linearly increased with the starvation time before the survivability significantly declined. This linearity was robust under various nutrient conditions and the recovery speed was predetermined by the initial nutrient condition. Transcriptome profiling further revealed that quiescence states under different nutrient conditions evolve in a common trajectory but with different speed. Our results demonstrated that cellular quiescence has a continuous spectrum of depths and its physiology is greatly influenced by environmental conditions. |
| first_indexed | 2024-03-07T18:36:31Z |
| format | Article |
| id | doaj.art-bb0ac4df40e14b7d8a59224368cf9aca |
| institution | Directory Open Access Journal |
| issn | 2405-8440 |
| language | English |
| last_indexed | 2024-04-24T23:16:33Z |
| publishDate | 2024-03-01 |
| publisher | Elsevier |
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| series | Heliyon |
| spelling | doaj.art-bb0ac4df40e14b7d8a59224368cf9aca2024-03-17T07:55:44ZengElsevierHeliyon2405-84402024-03-01105e26558Initial nutrient condition determines the recovery speed of quiescent cells in fission yeastQi Liu0Nan Sheng1Zhiwen Zhang2Chenjun He3Yao Zhao4Haoyuan Sun5Jianguo Chen6Xiaojing Yang7Chao Tang8Center for Quantitative Biology, Academy for Advanced Interdisciplinary Studies, Peking University, Beijing, 100871, China; The Key Laboratory of Cell Proliferation and Differentiation of Ministry of Education, School of Life Sciences, Peking University, Beijing, 100871, ChinaCenter for Quantitative Biology, Academy for Advanced Interdisciplinary Studies, Peking University, Beijing, 100871, ChinaPeking-Tsinghua Center for Life Sciences, Academy for Advanced Interdisciplinary Studies, Peking University, Beijing, 100871, ChinaCollege of Life Science and Technology, Huazhong Agriculture University, Wuhan, 430070, ChinaCenter for Quantitative Biology, Academy for Advanced Interdisciplinary Studies, Peking University, Beijing, 100871, ChinaPeking-Tsinghua Center for Life Sciences, Academy for Advanced Interdisciplinary Studies, Peking University, Beijing, 100871, ChinaCenter for Quantitative Biology, Academy for Advanced Interdisciplinary Studies, Peking University, Beijing, 100871, China; The Key Laboratory of Cell Proliferation and Differentiation of Ministry of Education, School of Life Sciences, Peking University, Beijing, 100871, ChinaCenter for Quantitative Biology, Academy for Advanced Interdisciplinary Studies, Peking University, Beijing, 100871, China; Corresponding author.Center for Quantitative Biology, Academy for Advanced Interdisciplinary Studies, Peking University, Beijing, 100871, China; Peking-Tsinghua Center for Life Sciences, Academy for Advanced Interdisciplinary Studies, Peking University, Beijing, 100871, China; School of Physics, Peking University, Beijing, 100871, China; Corresponding author. Center for Quantitative Biology, Academy for Advanced Interdisciplinary Studies, Peking University, Beijing 100871, China.Most of microbe cells spend the majority of their times in quiescence due to unfavorable environmental conditions. The study of this dominant state is crucial for understanding the basic cell physiology. Retained recovery ability is a critical property of quiescent cells, which consists of two features: how long the cells can survive (the survivability) and how fast they can recover (the recovery activity). While the survivability has been extensively studied under the background of chronological aging, how the recovery activity depends on the quiescent time and what factors influence its dynamics have not been addressed quantitatively. In this work, we systematically quantified both the survivability and the recovery activity of long-lived quiescent fission yeast cells at the single cell level under various nutrient conditions. It provides the most profound evolutionary dynamics of quiescent cell regeneration ability described to date. We found that the single cell recovery time linearly increased with the starvation time before the survivability significantly declined. This linearity was robust under various nutrient conditions and the recovery speed was predetermined by the initial nutrient condition. Transcriptome profiling further revealed that quiescence states under different nutrient conditions evolve in a common trajectory but with different speed. Our results demonstrated that cellular quiescence has a continuous spectrum of depths and its physiology is greatly influenced by environmental conditions.http://www.sciencedirect.com/science/article/pii/S2405844024025891Long-term quiescenceRecovery activityDynamicsFission yeastNitrogen starvationSingle-cell study |
| spellingShingle | Qi Liu Nan Sheng Zhiwen Zhang Chenjun He Yao Zhao Haoyuan Sun Jianguo Chen Xiaojing Yang Chao Tang Initial nutrient condition determines the recovery speed of quiescent cells in fission yeast Heliyon Long-term quiescence Recovery activity Dynamics Fission yeast Nitrogen starvation Single-cell study |
| title | Initial nutrient condition determines the recovery speed of quiescent cells in fission yeast |
| title_full | Initial nutrient condition determines the recovery speed of quiescent cells in fission yeast |
| title_fullStr | Initial nutrient condition determines the recovery speed of quiescent cells in fission yeast |
| title_full_unstemmed | Initial nutrient condition determines the recovery speed of quiescent cells in fission yeast |
| title_short | Initial nutrient condition determines the recovery speed of quiescent cells in fission yeast |
| title_sort | initial nutrient condition determines the recovery speed of quiescent cells in fission yeast |
| topic | Long-term quiescence Recovery activity Dynamics Fission yeast Nitrogen starvation Single-cell study |
| url | http://www.sciencedirect.com/science/article/pii/S2405844024025891 |
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