Production and characterization of pectinase enzyme from rhizopus oryzae
<p>The research was conducted with the production of pectinase enzyme by fungal fermentation using <em>Rhizopus oryzae</em> and characterization of produced enzyme with respect to pH, temperature, incubation time, and substrate specificity. Carbon source was optimized replacing sug...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
HACCP Consulting
2017-01-01
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| Series: | Potravinarstvo |
| Subjects: | |
| Online Access: | http://www.potravinarstvo.com/journal1/index.php/potravinarstvo/article/view/656 |
| _version_ | 1811319724412239872 |
|---|---|
| author | Taminur Islam Chowdhury Md. Fahad Jubayer Md. Burhan Uddin Md. Gulzarul Aziz |
| author_facet | Taminur Islam Chowdhury Md. Fahad Jubayer Md. Burhan Uddin Md. Gulzarul Aziz |
| author_sort | Taminur Islam Chowdhury |
| collection | DOAJ |
| description | <p>The research was conducted with the production of pectinase enzyme by fungal fermentation using <em>Rhizopus oryzae</em> and characterization of produced enzyme with respect to pH, temperature, incubation time, and substrate specificity. Carbon source was optimized replacing sugar with different concentration of pectin (0.5, 0.75, and 1.0%) during submerged fermentation. The outcomes of the fermentation process showed that media containing carbon source of 1% pectin replaced dextrose at pH 6.0 incubating for 72 hours at 35 °C were the best condition for pectinase production. The maximal activity for pectinase enzyme produced from <em>Rhizopus oryzae</em> by fermentation was 3.16 U.mL<sup>-1</sup> and it was found at 40 °C and pH 6.5. The produced pectinase enzyme was found thermo stable up to 60 °C for 50 min. The activity of the enzyme was increased with the increasing pectin concentration in the media and maximum activity was found at the pectin concentration of 5 mg.mL<sup>-1</sup>. The k<sub>m</sub> and V<sub>max</sub> values were found 0.84 mg.mL<sup>-1</sup> and 5.294 mg.mL<sup>-1</sup> respectively at optimized condition. The outcomes of the research may be useful for further research in low cost production of pectinases from <em>R.oryzae</em> for domestic consumption in many industries.</p> <!--[if gte mso 9]><xml> <w:WordDocument> <w:View>Normal</w:View> <w:Zoom>0</w:Zoom> <w:TrackMoves /> <w:TrackFormatting /> <w:PunctuationKerning /> <w:ValidateAgainstSchemas /> <w:SaveIfXMLInvalid>false</w:SaveIfXMLInvalid> <w:IgnoreMixedContent>false</w:IgnoreMixedContent> <w:AlwaysShowPlaceholderText>false</w:AlwaysShowPlaceholderText> <w:DoNotPromoteQF /> <w:LidThemeOther>EN-US</w:LidThemeOther> <w:LidThemeAsian>X-NONE</w:LidThemeAsian> <w:LidThemeComplexScript>BN</w:LidThemeComplexScript> <w:Compatibility> <w:BreakWrappedTables /> <w:SnapToGridInCell /> <w:WrapTextWithPunct /> <w:UseAsianBreakRules /> <w:DontGrowAutofit /> <w:SplitPgBreakAndParaMark /> <w:DontVertAlignCellWithSp /> <w:DontBreakConstrainedForcedTables /> <w:DontVertAlignInTxbx /> <w:Word11KerningPairs /> <w:CachedColBalance /> </w:Compatibility> 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| first_indexed | 2024-04-13T12:47:40Z |
| format | Article |
| id | doaj.art-bb0b54ea67c244e9ac0ac025354f9f00 |
| institution | Directory Open Access Journal |
| issn | 1337-0960 |
| language | English |
| last_indexed | 2024-04-13T12:47:40Z |
| publishDate | 2017-01-01 |
| publisher | HACCP Consulting |
| record_format | Article |
| series | Potravinarstvo |
| spelling | doaj.art-bb0b54ea67c244e9ac0ac025354f9f002022-12-22T02:46:19ZengHACCP ConsultingPotravinarstvo1337-09602017-01-0111164165110.5219/656606Production and characterization of pectinase enzyme from rhizopus oryzaeTaminur Islam Chowdhury0Md. Fahad Jubayer1Md. Burhan Uddin2Md. Gulzarul Aziz3Department of Food Technology and Rural Industries, Bangladesh Agricultural University, Mymensingh-2202Department of Food Technology and Rural Industries, Bangladesh Agricultural University, Mymensingh-2202Department of Food Technology and Rural Industries, Bangladesh Agricultural University, Mymensingh-2202Department of Food Technology and Rural Industries, Bangladesh Agricultural University, Mymensingh-2202<p>The research was conducted with the production of pectinase enzyme by fungal fermentation using <em>Rhizopus oryzae</em> and characterization of produced enzyme with respect to pH, temperature, incubation time, and substrate specificity. Carbon source was optimized replacing sugar with different concentration of pectin (0.5, 0.75, and 1.0%) during submerged fermentation. The outcomes of the fermentation process showed that media containing carbon source of 1% pectin replaced dextrose at pH 6.0 incubating for 72 hours at 35 °C were the best condition for pectinase production. The maximal activity for pectinase enzyme produced from <em>Rhizopus oryzae</em> by fermentation was 3.16 U.mL<sup>-1</sup> and it was found at 40 °C and pH 6.5. The produced pectinase enzyme was found thermo stable up to 60 °C for 50 min. The activity of the enzyme was increased with the increasing pectin concentration in the media and maximum activity was found at the pectin concentration of 5 mg.mL<sup>-1</sup>. The k<sub>m</sub> and V<sub>max</sub> values were found 0.84 mg.mL<sup>-1</sup> and 5.294 mg.mL<sup>-1</sup> respectively at optimized condition. The outcomes of the research may be useful for further research in low cost production of pectinases from <em>R.oryzae</em> for domestic consumption in many industries.</p> <!--[if gte mso 9]><xml> <w:WordDocument> <w:View>Normal</w:View> <w:Zoom>0</w:Zoom> <w:TrackMoves /> <w:TrackFormatting /> <w:PunctuationKerning /> <w:ValidateAgainstSchemas /> <w:SaveIfXMLInvalid>false</w:SaveIfXMLInvalid> <w:IgnoreMixedContent>false</w:IgnoreMixedContent> <w:AlwaysShowPlaceholderText>false</w:AlwaysShowPlaceholderText> <w:DoNotPromoteQF /> <w:LidThemeOther>EN-US</w:LidThemeOther> <w:LidThemeAsian>X-NONE</w:LidThemeAsian> <w:LidThemeComplexScript>BN</w:LidThemeComplexScript> <w:Compatibility> <w:BreakWrappedTables /> <w:SnapToGridInCell /> <w:WrapTextWithPunct /> <w:UseAsianBreakRules /> <w:DontGrowAutofit /> <w:SplitPgBreakAndParaMark /> <w:DontVertAlignCellWithSp /> <w:DontBreakConstrainedForcedTables /> <w:DontVertAlignInTxbx /> <w:Word11KerningPairs /> <w:CachedColBalance /> </w:Compatibility> 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mso-tstyle-rowband-size:0; mso-tstyle-colband-size:0; mso-style-noshow:yes; mso-style-priority:99; mso-style-qformat:yes; mso-style-parent:""; mso-padding-alt:0in 5.4pt 0in 5.4pt; mso-para-margin:0in; mso-para-margin-bottom:.0001pt; mso-pagination:widow-orphan; font-size:11.0pt; mso-bidi-font-size:14.0pt; font-family:"Calibri","sans-serif"; mso-ascii-font-family:Calibri; mso-ascii-theme-font:minor-latin; mso-fareast-font-family:"Times New Roman"; mso-fareast-theme-font:minor-fareast; mso-hansi-font-family:Calibri; mso-hansi-theme-font:minor-latin; mso-bidi-font-family:Vrinda; mso-bidi-theme-font:minor-bidi;} --> <!--[endif] -->http://www.potravinarstvo.com/journal1/index.php/potravinarstvo/article/view/656pectinasesubmerged fermentationPectinenzymeincubation |
| spellingShingle | Taminur Islam Chowdhury Md. Fahad Jubayer Md. Burhan Uddin Md. Gulzarul Aziz Production and characterization of pectinase enzyme from rhizopus oryzae Potravinarstvo pectinase submerged fermentation Pectin enzyme incubation |
| title | Production and characterization of pectinase enzyme from rhizopus oryzae |
| title_full | Production and characterization of pectinase enzyme from rhizopus oryzae |
| title_fullStr | Production and characterization of pectinase enzyme from rhizopus oryzae |
| title_full_unstemmed | Production and characterization of pectinase enzyme from rhizopus oryzae |
| title_short | Production and characterization of pectinase enzyme from rhizopus oryzae |
| title_sort | production and characterization of pectinase enzyme from rhizopus oryzae |
| topic | pectinase submerged fermentation Pectin enzyme incubation |
| url | http://www.potravinarstvo.com/journal1/index.php/potravinarstvo/article/view/656 |
| work_keys_str_mv | AT taminurislamchowdhury productionandcharacterizationofpectinaseenzymefromrhizopusoryzae AT mdfahadjubayer productionandcharacterizationofpectinaseenzymefromrhizopusoryzae AT mdburhanuddin productionandcharacterizationofpectinaseenzymefromrhizopusoryzae AT mdgulzarulaziz productionandcharacterizationofpectinaseenzymefromrhizopusoryzae |