Detection of genetic material of causative agents of animal viral diseases in blood-sucking dipterans from the Tyumen Region
The purpose of the research is to test blood-sucking dipterans collected in the Tyumen Region for genetic material of viruses that cause dangerous diseases in live-stock animals.Materials and methods. From May to October 2021, blood-sucking insects of the Diptera order whose species membership was e...
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Federal Scientific Centre VIEV
2023-01-01
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| Series: | Российский паразитологический журнал |
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| Online Access: | https://vniigis.elpub.ru/jour/article/view/965 |
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| author | K. S. Krutko A. G. Kinareikina M. I. Serkova E. A. Silivanova O. A. Fedorova |
| author_facet | K. S. Krutko A. G. Kinareikina M. I. Serkova E. A. Silivanova O. A. Fedorova |
| author_sort | K. S. Krutko |
| collection | DOAJ |
| description | The purpose of the research is to test blood-sucking dipterans collected in the Tyumen Region for genetic material of viruses that cause dangerous diseases in live-stock animals.Materials and methods. From May to October 2021, blood-sucking insects of the Diptera order whose species membership was established by tabular keys were collected on pastures and farms in ten Tyumen Region districts. In 60 samples formed from the captured insects according to the taxonomic affiliation and the period and place of collection, the presence of genetic material of the leukemia provirus and the dermatitis nodularis virus of cattle (bovine) and the African swine fever virus was evaluated by polymerase chain reaction (PCR) in real time.Results and discussion. Adult insects collected for the PCR analysis were blood-sucking flies (family Muscidae, genus Stomoxys), mosquitoes (family Culicidae, genus Aedes), midges (family Simuliidae, genera Byssodon and Schoenbaueria), horseflies (family Tabanidae, genera Hybomitra, Tabanus and Haematopota), and biting midges (family Ceratopogonidae, genus Culicoides). As a result of the PCR testing of the samples for the bovine leukemia provirus DNA, 1 out of 13 samples of Stomoxys spp. (7.7%) and 1 of 13 samples of Hybomitra spp. (7.7%) were positive. The bovine leukemia provirus DNA detected in blood-sucking insects indicates the presence of this pathogen in the insect collection area as well as their possible involvement in its spread. Further research is needed on the Stomoxys spp. and Hybomitra spp. vector competence in vivo, considering natural and climatic features of the Tyumen Region. |
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| institution | Directory Open Access Journal |
| issn | 1998-8435 2541-7843 |
| language | Russian |
| last_indexed | 2025-03-14T11:48:29Z |
| publishDate | 2023-01-01 |
| publisher | Federal Scientific Centre VIEV |
| record_format | Article |
| series | Российский паразитологический журнал |
| spelling | doaj.art-bb0b8ef6249a4326b6b3159460adc73e2025-03-02T09:49:59ZrusFederal Scientific Centre VIEVРоссийский паразитологический журнал1998-84352541-78432023-01-0116438940210.31016/1998-8435-2022-16-4-389-402712Detection of genetic material of causative agents of animal viral diseases in blood-sucking dipterans from the Tyumen RegionK. S. Krutko0A. G. Kinareikina1M. I. Serkova2E. A. Silivanova3O. A. Fedorova4All-Russian Scientific Research Institute of Veterinary Entomology and Arachnology – Branch of Federal State Institution Federal Research Centre Tyumen Scientific Centre of Siberian Branch of the Russian Academy of Sciences (ASRIVEA – Branch of Tyumen Scientific Centre SB RAS)All-Russian Scientific Research Institute of Veterinary Entomology and Arachnology – Branch of Federal State Institution Federal Research Centre Tyumen Scientific Centre of Siberian Branch of the Russian Academy of Sciences (ASRIVEA – Branch of Tyumen Scientific Centre SB RAS)All-Russian Scientific Research Institute of Veterinary Entomology and Arachnology – Branch of Federal State Institution Federal Research Centre Tyumen Scientific Centre of Siberian Branch of the Russian Academy of Sciences (ASRIVEA – Branch of Tyumen Scientific Centre SB RAS)All-Russian Scientific Research Institute of Veterinary Entomology and Arachnology – Branch of Federal State Institution Federal Research Centre Tyumen Scientific Centre of Siberian Branch of the Russian Academy of Sciences (ASRIVEA – Branch of Tyumen Scientific Centre SB RAS)All-Russian Scientific Research Institute of Veterinary Entomology and Arachnology – Branch of Federal State Institution Federal Research Centre Tyumen Scientific Centre of Siberian Branch of the Russian Academy of Sciences (ASRIVEA – Branch of Tyumen Scientific Centre SB RAS)The purpose of the research is to test blood-sucking dipterans collected in the Tyumen Region for genetic material of viruses that cause dangerous diseases in live-stock animals.Materials and methods. From May to October 2021, blood-sucking insects of the Diptera order whose species membership was established by tabular keys were collected on pastures and farms in ten Tyumen Region districts. In 60 samples formed from the captured insects according to the taxonomic affiliation and the period and place of collection, the presence of genetic material of the leukemia provirus and the dermatitis nodularis virus of cattle (bovine) and the African swine fever virus was evaluated by polymerase chain reaction (PCR) in real time.Results and discussion. Adult insects collected for the PCR analysis were blood-sucking flies (family Muscidae, genus Stomoxys), mosquitoes (family Culicidae, genus Aedes), midges (family Simuliidae, genera Byssodon and Schoenbaueria), horseflies (family Tabanidae, genera Hybomitra, Tabanus and Haematopota), and biting midges (family Ceratopogonidae, genus Culicoides). As a result of the PCR testing of the samples for the bovine leukemia provirus DNA, 1 out of 13 samples of Stomoxys spp. (7.7%) and 1 of 13 samples of Hybomitra spp. (7.7%) were positive. The bovine leukemia provirus DNA detected in blood-sucking insects indicates the presence of this pathogen in the insect collection area as well as their possible involvement in its spread. Further research is needed on the Stomoxys spp. and Hybomitra spp. vector competence in vivo, considering natural and climatic features of the Tyumen Region.https://vniigis.elpub.ru/jour/article/view/965vector-borne diseasesleukemiacattleinfectious dermatitis nodularisafrican swine feverhematophagousdiptera |
| spellingShingle | K. S. Krutko A. G. Kinareikina M. I. Serkova E. A. Silivanova O. A. Fedorova Detection of genetic material of causative agents of animal viral diseases in blood-sucking dipterans from the Tyumen Region Российский паразитологический журнал vector-borne diseases leukemia cattle infectious dermatitis nodularis african swine fever hematophagous diptera |
| title | Detection of genetic material of causative agents of animal viral diseases in blood-sucking dipterans from the Tyumen Region |
| title_full | Detection of genetic material of causative agents of animal viral diseases in blood-sucking dipterans from the Tyumen Region |
| title_fullStr | Detection of genetic material of causative agents of animal viral diseases in blood-sucking dipterans from the Tyumen Region |
| title_full_unstemmed | Detection of genetic material of causative agents of animal viral diseases in blood-sucking dipterans from the Tyumen Region |
| title_short | Detection of genetic material of causative agents of animal viral diseases in blood-sucking dipterans from the Tyumen Region |
| title_sort | detection of genetic material of causative agents of animal viral diseases in blood sucking dipterans from the tyumen region |
| topic | vector-borne diseases leukemia cattle infectious dermatitis nodularis african swine fever hematophagous diptera |
| url | https://vniigis.elpub.ru/jour/article/view/965 |
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