The Effect of Infertility on the Liver Structure, Endocrinology, and Gene Network in Japanese Flounder
The liver can synthesize vitellogenin, the precursor of vitellin, which is needed for oocyte development and maturation. Here, we investigated the effects of infertility on liver structure, hormone regulation, and gene and protein networks in Japanese flounder (<i>Paralichthys olivaceus</i&...
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MDPI AG
2021-03-01
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| Online Access: | https://www.mdpi.com/2076-2615/11/4/936 |
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| author | Qi Mang Jilun Hou Tian Han Guixing Wang Yufen Wang Yufeng Liu Yuqin Ren Yaxian Zhao Zhongwei He Xiaoyan Zhang |
| author_facet | Qi Mang Jilun Hou Tian Han Guixing Wang Yufen Wang Yufeng Liu Yuqin Ren Yaxian Zhao Zhongwei He Xiaoyan Zhang |
| author_sort | Qi Mang |
| collection | DOAJ |
| description | The liver can synthesize vitellogenin, the precursor of vitellin, which is needed for oocyte development and maturation. Here, we investigated the effects of infertility on liver structure, hormone regulation, and gene and protein networks in Japanese flounder (<i>Paralichthys olivaceus</i>). Results showed that the liver of infertile fish had fewer vacuoles and significantly lower serum vitellogenin (VTG) level than in liver of fertile fish. Whole transcriptomics analysis between infertile and fertile groups identified 2076 significantly differentially expressed (DE) mRNAs, 431 DE lncRNAs, 265 DE circRNAs, and 53 DE miRNAs. Proteomics analysis identified 838 DE proteins. Integrated analysis of whole transcriptomics and proteomics revealed 60 significantly DE genes and proteins associated with metabolism, immunity, signal transduction, and steroid biosynthesis. Moreover, non-coding RNA (miRNAs, circRNA, and lncRNA) transcripts involved in metabolism, immunity, and signal transduction in infertile liver were identified. In conclusion, this study shows that gonadal infertility is associated with not only changes in histological structure and hormone secretion but also changes in metabolism, immunity, and signal transduction networks in the liver. These results provide valuable information concerning the mechanism underlying infertility in fish. |
| first_indexed | 2024-03-10T12:54:31Z |
| format | Article |
| id | doaj.art-bb0e464b152e4de9902fc822cfaa4b7c |
| institution | Directory Open Access Journal |
| issn | 2076-2615 |
| language | English |
| last_indexed | 2024-03-10T12:54:31Z |
| publishDate | 2021-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Animals |
| spelling | doaj.art-bb0e464b152e4de9902fc822cfaa4b7c2023-11-21T12:03:24ZengMDPI AGAnimals2076-26152021-03-0111493610.3390/ani11040936The Effect of Infertility on the Liver Structure, Endocrinology, and Gene Network in Japanese FlounderQi Mang0Jilun Hou1Tian Han2Guixing Wang3Yufen Wang4Yufeng Liu5Yuqin Ren6Yaxian Zhao7Zhongwei He8Xiaoyan Zhang9Key Laboratory of Aquatic Genomics, Ministry of Agriculture, Beijing 100141, ChinaKey Laboratory of Aquatic Genomics, Ministry of Agriculture, Beijing 100141, ChinaBeidaihe Central Experiment Station, Chinese Academy of Fishery Sciences, Qinhuangdao 066100, ChinaBeidaihe Central Experiment Station, Chinese Academy of Fishery Sciences, Qinhuangdao 066100, ChinaBeidaihe Central Experiment Station, Chinese Academy of Fishery Sciences, Qinhuangdao 066100, ChinaBeidaihe Central Experiment Station, Chinese Academy of Fishery Sciences, Qinhuangdao 066100, ChinaBeidaihe Central Experiment Station, Chinese Academy of Fishery Sciences, Qinhuangdao 066100, ChinaBeidaihe Central Experiment Station, Chinese Academy of Fishery Sciences, Qinhuangdao 066100, ChinaBeidaihe Central Experiment Station, Chinese Academy of Fishery Sciences, Qinhuangdao 066100, ChinaKey Laboratory of Aquatic Genomics, Ministry of Agriculture, Beijing 100141, ChinaThe liver can synthesize vitellogenin, the precursor of vitellin, which is needed for oocyte development and maturation. Here, we investigated the effects of infertility on liver structure, hormone regulation, and gene and protein networks in Japanese flounder (<i>Paralichthys olivaceus</i>). Results showed that the liver of infertile fish had fewer vacuoles and significantly lower serum vitellogenin (VTG) level than in liver of fertile fish. Whole transcriptomics analysis between infertile and fertile groups identified 2076 significantly differentially expressed (DE) mRNAs, 431 DE lncRNAs, 265 DE circRNAs, and 53 DE miRNAs. Proteomics analysis identified 838 DE proteins. Integrated analysis of whole transcriptomics and proteomics revealed 60 significantly DE genes and proteins associated with metabolism, immunity, signal transduction, and steroid biosynthesis. Moreover, non-coding RNA (miRNAs, circRNA, and lncRNA) transcripts involved in metabolism, immunity, and signal transduction in infertile liver were identified. In conclusion, this study shows that gonadal infertility is associated with not only changes in histological structure and hormone secretion but also changes in metabolism, immunity, and signal transduction networks in the liver. These results provide valuable information concerning the mechanism underlying infertility in fish.https://www.mdpi.com/2076-2615/11/4/936<i>Paralichthys olivaceus</i>doubled haploidinfertilevitellogeninceRNA |
| spellingShingle | Qi Mang Jilun Hou Tian Han Guixing Wang Yufen Wang Yufeng Liu Yuqin Ren Yaxian Zhao Zhongwei He Xiaoyan Zhang The Effect of Infertility on the Liver Structure, Endocrinology, and Gene Network in Japanese Flounder Animals <i>Paralichthys olivaceus</i> doubled haploid infertile vitellogenin ceRNA |
| title | The Effect of Infertility on the Liver Structure, Endocrinology, and Gene Network in Japanese Flounder |
| title_full | The Effect of Infertility on the Liver Structure, Endocrinology, and Gene Network in Japanese Flounder |
| title_fullStr | The Effect of Infertility on the Liver Structure, Endocrinology, and Gene Network in Japanese Flounder |
| title_full_unstemmed | The Effect of Infertility on the Liver Structure, Endocrinology, and Gene Network in Japanese Flounder |
| title_short | The Effect of Infertility on the Liver Structure, Endocrinology, and Gene Network in Japanese Flounder |
| title_sort | effect of infertility on the liver structure endocrinology and gene network in japanese flounder |
| topic | <i>Paralichthys olivaceus</i> doubled haploid infertile vitellogenin ceRNA |
| url | https://www.mdpi.com/2076-2615/11/4/936 |
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