Cytotoxic Effects on Gingival Mesenchymal Stromal Cells and Root Surface Modifications Induced by Some Local Antimicrobial Products Used in Periodontitis Treatment
(1) Background: this study aims to test the cytotoxicity of three antimicrobial products used in periodontitis treatment on gingival mesenchymal stem cells (gMSCs) and their influence on root surfaces and gMSC adhesion. We tested the null hypothesis that the effects of the antimicrobials did not dif...
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| Online Access: | https://www.mdpi.com/1996-1944/14/17/5049 |
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| author | Irina Lupșe Emoke Pall Lucian Barbu Tudoran Adriana Elena Bulboacă Andreea Ciurea Iulia Cristina Micu Alexandra Roman Ada Gabriela Delean Alexandrina Muntean Andrada Soancă |
| author_facet | Irina Lupșe Emoke Pall Lucian Barbu Tudoran Adriana Elena Bulboacă Andreea Ciurea Iulia Cristina Micu Alexandra Roman Ada Gabriela Delean Alexandrina Muntean Andrada Soancă |
| author_sort | Irina Lupșe |
| collection | DOAJ |
| description | (1) Background: this study aims to test the cytotoxicity of three antimicrobial products used in periodontitis treatment on gingival mesenchymal stem cells (gMSCs) and their influence on root surfaces and gMSC adhesion. We tested the null hypothesis that the effects of the antimicrobials did not differ. (2) Methods: the commercial products based on sulphonic/sulphuric acids, sodium hypochlorite and silver nanoparticles, in five different concentrations, were added to culture medium for growing gMSCs. Cell proliferation capacity was tested using the Cell Counting Kit-8 (CCK8) and their viability was determined by succinate dehydrogenase activity (MTT) assay. Scanning electron microscopy evaluated the adhesion of gMSCs on root samples treated mechanically and with commercial products. (3) Results: the products induced a dose-dependent cytotoxicity in terms of reduced proliferation and viability of gMSCs, as well as cell shape modifications. Significant differences in CCK8 values between the different commercial products were observed. Based on proliferation tests, the null hypothesis was rejected. When MTT values of the three products were compared with each other, no significant differences were observed for any of the five concentrations (<i>p</i> = 0.065, <i>p</i> = 0.067, <i>p</i> = 0.172, <i>p</i> = 0.256, <i>p</i> = 0.060). (4) Conclusions: the three antimicrobials had a certain degree of cytotoxicity on gMSCs. gMSCs repopulated treated root surfaces. |
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| issn | 1996-1944 |
| language | English |
| last_indexed | 2024-03-10T08:08:09Z |
| publishDate | 2021-09-01 |
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| record_format | Article |
| series | Materials |
| spelling | doaj.art-bb11538b7fb44723968fc9a5366bc85d2023-11-22T10:55:35ZengMDPI AGMaterials1996-19442021-09-011417504910.3390/ma14175049Cytotoxic Effects on Gingival Mesenchymal Stromal Cells and Root Surface Modifications Induced by Some Local Antimicrobial Products Used in Periodontitis TreatmentIrina Lupșe0Emoke Pall1Lucian Barbu Tudoran2Adriana Elena Bulboacă3Andreea Ciurea4Iulia Cristina Micu5Alexandra Roman6Ada Gabriela Delean7Alexandrina Muntean8Andrada Soancă9Department of Paediatric Dentistry, Faculty of Dental Medicine, Iuliu Hațieganu University of Medicine and Pharmacy, Avram Iancu St., No. 31, 400083 Cluj-Napoca, RomaniaDepartment of Infectious Disease, Faculty of Veterinary Medicine, University of Agricultural Sciences and Veterinary Medicine, 3-5 Mănăștur St., 400372 Cluj-Napoca, RomaniaDepartment of Molecular Biology and Biotechnologies, Faculty of Biology and Geology, Babeş-Bolyai University, Clinicilor St., No. 5-7, 400006 Cluj-Napoca, RomaniaDepartment of Pathophysiology, Iuliu Hațieganu University of Medicine and Pharmacy, 4-6 Victor Babeș St., 400012 Cluj-Napoca, RomaniaDepartment of Periodontology, Faculty of Dental Medicine, Iuliu Haţieganu University of Medicine and Pharmacy, Victor Babeş St., No. 15, 400012 Cluj-Napoca, RomaniaDepartment of Periodontology, Faculty of Dental Medicine, Iuliu Haţieganu University of Medicine and Pharmacy, Victor Babeş St., No. 15, 400012 Cluj-Napoca, RomaniaDepartment of Periodontology, Faculty of Dental Medicine, Iuliu Haţieganu University of Medicine and Pharmacy, Victor Babeş St., No. 15, 400012 Cluj-Napoca, RomaniaDepartment of Odontology and Endodontics, Faculty of Dental Medicine, Iuliu Haţieganu University of Medicine and Pharmacy, 400012 Cluj-Napoca, RomaniaDepartment of Paediatric Dentistry, Faculty of Dental Medicine, Iuliu Hațieganu University of Medicine and Pharmacy, Avram Iancu St., No. 31, 400083 Cluj-Napoca, RomaniaDepartment of Periodontology, Faculty of Dental Medicine, Iuliu Haţieganu University of Medicine and Pharmacy, Victor Babeş St., No. 15, 400012 Cluj-Napoca, Romania(1) Background: this study aims to test the cytotoxicity of three antimicrobial products used in periodontitis treatment on gingival mesenchymal stem cells (gMSCs) and their influence on root surfaces and gMSC adhesion. We tested the null hypothesis that the effects of the antimicrobials did not differ. (2) Methods: the commercial products based on sulphonic/sulphuric acids, sodium hypochlorite and silver nanoparticles, in five different concentrations, were added to culture medium for growing gMSCs. Cell proliferation capacity was tested using the Cell Counting Kit-8 (CCK8) and their viability was determined by succinate dehydrogenase activity (MTT) assay. Scanning electron microscopy evaluated the adhesion of gMSCs on root samples treated mechanically and with commercial products. (3) Results: the products induced a dose-dependent cytotoxicity in terms of reduced proliferation and viability of gMSCs, as well as cell shape modifications. Significant differences in CCK8 values between the different commercial products were observed. Based on proliferation tests, the null hypothesis was rejected. When MTT values of the three products were compared with each other, no significant differences were observed for any of the five concentrations (<i>p</i> = 0.065, <i>p</i> = 0.067, <i>p</i> = 0.172, <i>p</i> = 0.256, <i>p</i> = 0.060). (4) Conclusions: the three antimicrobials had a certain degree of cytotoxicity on gMSCs. gMSCs repopulated treated root surfaces.https://www.mdpi.com/1996-1944/14/17/5049stem celladhesiondental disinfectantsmear layertooth root |
| spellingShingle | Irina Lupșe Emoke Pall Lucian Barbu Tudoran Adriana Elena Bulboacă Andreea Ciurea Iulia Cristina Micu Alexandra Roman Ada Gabriela Delean Alexandrina Muntean Andrada Soancă Cytotoxic Effects on Gingival Mesenchymal Stromal Cells and Root Surface Modifications Induced by Some Local Antimicrobial Products Used in Periodontitis Treatment Materials stem cell adhesion dental disinfectant smear layer tooth root |
| title | Cytotoxic Effects on Gingival Mesenchymal Stromal Cells and Root Surface Modifications Induced by Some Local Antimicrobial Products Used in Periodontitis Treatment |
| title_full | Cytotoxic Effects on Gingival Mesenchymal Stromal Cells and Root Surface Modifications Induced by Some Local Antimicrobial Products Used in Periodontitis Treatment |
| title_fullStr | Cytotoxic Effects on Gingival Mesenchymal Stromal Cells and Root Surface Modifications Induced by Some Local Antimicrobial Products Used in Periodontitis Treatment |
| title_full_unstemmed | Cytotoxic Effects on Gingival Mesenchymal Stromal Cells and Root Surface Modifications Induced by Some Local Antimicrobial Products Used in Periodontitis Treatment |
| title_short | Cytotoxic Effects on Gingival Mesenchymal Stromal Cells and Root Surface Modifications Induced by Some Local Antimicrobial Products Used in Periodontitis Treatment |
| title_sort | cytotoxic effects on gingival mesenchymal stromal cells and root surface modifications induced by some local antimicrobial products used in periodontitis treatment |
| topic | stem cell adhesion dental disinfectant smear layer tooth root |
| url | https://www.mdpi.com/1996-1944/14/17/5049 |
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