Bioactive Compounds in Wild Nettle (<i>Urtica dioica</i> L.) Leaves and Stalks: Polyphenols and Pigments upon Seasonal and Habitat Variations
This study evaluated the presence of bioactives in wild nettle leaves and stalks during the phenological stage and in the context of natural habitat diversity. Thus, wild nettle samples collected before flowering, during flowering and after flowering from 14 habitats situated in three different regi...
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-01-01
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| Series: | Foods |
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| Online Access: | https://www.mdpi.com/2304-8158/10/1/190 |
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| author | Maja Repajić Ena Cegledi Zoran Zorić Sandra Pedisić Ivona Elez Garofulić Sanja Radman Igor Palčić Verica Dragović-Uzelac |
| author_facet | Maja Repajić Ena Cegledi Zoran Zorić Sandra Pedisić Ivona Elez Garofulić Sanja Radman Igor Palčić Verica Dragović-Uzelac |
| author_sort | Maja Repajić |
| collection | DOAJ |
| description | This study evaluated the presence of bioactives in wild nettle leaves and stalks during the phenological stage and in the context of natural habitat diversity. Thus, wild nettle samples collected before flowering, during flowering and after flowering from 14 habitats situated in three different regions (continental, mountain and seaside) were analyzed for low molecular weight polyphenols, carotenoids and chlorophylls using UPLC-MS/MS and HPLC analysis, while the ORAC method was performed for the antioxidant capacity measurement. Statistical analysis showed that, when compared to the stalks, nettle leaves contained significantly higher amounts of analyzed compounds which accumulated in the highest yields before flowering (polyphenols) and at the flowering stage (pigments). Moreover, nettle habitat variations greatly influenced the amounts of analyzed bioactives, where samples from the continental area contained higher levels of polyphenols, while seaside region samples were more abundant with pigments. The levels of ORAC followed the same pattern, being higher in leaves samples collected before and during flowering from the continental habitats. Hence, in order to provide the product’s maximum value for consumers’ benefit, a multidisciplinary approach is important for the selection of a plant part as well as its phenological stage with the highest accumulation of bioactive compounds. |
| first_indexed | 2024-03-09T04:24:12Z |
| format | Article |
| id | doaj.art-bb1174203d264a7e895440f5d0c64da7 |
| institution | Directory Open Access Journal |
| issn | 2304-8158 |
| language | English |
| last_indexed | 2024-03-09T04:24:12Z |
| publishDate | 2021-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Foods |
| spelling | doaj.art-bb1174203d264a7e895440f5d0c64da72023-12-03T13:43:33ZengMDPI AGFoods2304-81582021-01-0110119010.3390/foods10010190Bioactive Compounds in Wild Nettle (<i>Urtica dioica</i> L.) Leaves and Stalks: Polyphenols and Pigments upon Seasonal and Habitat VariationsMaja Repajić0Ena Cegledi1Zoran Zorić2Sandra Pedisić3Ivona Elez Garofulić4Sanja Radman5Igor Palčić6Verica Dragović-Uzelac7Faculty of Food Technology and Biotechnology, University of Zagreb Pierottijeva 6, 10000 Zagreb, CroatiaFaculty of Food Technology and Biotechnology, University of Zagreb Pierottijeva 6, 10000 Zagreb, CroatiaFaculty of Food Technology and Biotechnology, University of Zagreb Pierottijeva 6, 10000 Zagreb, CroatiaFaculty of Food Technology and Biotechnology, University of Zagreb Pierottijeva 6, 10000 Zagreb, CroatiaFaculty of Food Technology and Biotechnology, University of Zagreb Pierottijeva 6, 10000 Zagreb, CroatiaFaculty of Agriculture, University of Zagreb, Svetošimunska cesta 25, 10000 Zagreb, CroatiaInstitute of Agriculture and Tourism, Karla Huguesa 8, 52440 Poreč, CroatiaFaculty of Food Technology and Biotechnology, University of Zagreb Pierottijeva 6, 10000 Zagreb, CroatiaThis study evaluated the presence of bioactives in wild nettle leaves and stalks during the phenological stage and in the context of natural habitat diversity. Thus, wild nettle samples collected before flowering, during flowering and after flowering from 14 habitats situated in three different regions (continental, mountain and seaside) were analyzed for low molecular weight polyphenols, carotenoids and chlorophylls using UPLC-MS/MS and HPLC analysis, while the ORAC method was performed for the antioxidant capacity measurement. Statistical analysis showed that, when compared to the stalks, nettle leaves contained significantly higher amounts of analyzed compounds which accumulated in the highest yields before flowering (polyphenols) and at the flowering stage (pigments). Moreover, nettle habitat variations greatly influenced the amounts of analyzed bioactives, where samples from the continental area contained higher levels of polyphenols, while seaside region samples were more abundant with pigments. The levels of ORAC followed the same pattern, being higher in leaves samples collected before and during flowering from the continental habitats. Hence, in order to provide the product’s maximum value for consumers’ benefit, a multidisciplinary approach is important for the selection of a plant part as well as its phenological stage with the highest accumulation of bioactive compounds.https://www.mdpi.com/2304-8158/10/1/190nettle leaves and stalksphenological stagelocationaccelerated solvent extractionUPLC-MS/MSpolyphenols |
| spellingShingle | Maja Repajić Ena Cegledi Zoran Zorić Sandra Pedisić Ivona Elez Garofulić Sanja Radman Igor Palčić Verica Dragović-Uzelac Bioactive Compounds in Wild Nettle (<i>Urtica dioica</i> L.) Leaves and Stalks: Polyphenols and Pigments upon Seasonal and Habitat Variations Foods nettle leaves and stalks phenological stage location accelerated solvent extraction UPLC-MS/MS polyphenols |
| title | Bioactive Compounds in Wild Nettle (<i>Urtica dioica</i> L.) Leaves and Stalks: Polyphenols and Pigments upon Seasonal and Habitat Variations |
| title_full | Bioactive Compounds in Wild Nettle (<i>Urtica dioica</i> L.) Leaves and Stalks: Polyphenols and Pigments upon Seasonal and Habitat Variations |
| title_fullStr | Bioactive Compounds in Wild Nettle (<i>Urtica dioica</i> L.) Leaves and Stalks: Polyphenols and Pigments upon Seasonal and Habitat Variations |
| title_full_unstemmed | Bioactive Compounds in Wild Nettle (<i>Urtica dioica</i> L.) Leaves and Stalks: Polyphenols and Pigments upon Seasonal and Habitat Variations |
| title_short | Bioactive Compounds in Wild Nettle (<i>Urtica dioica</i> L.) Leaves and Stalks: Polyphenols and Pigments upon Seasonal and Habitat Variations |
| title_sort | bioactive compounds in wild nettle i urtica dioica i l leaves and stalks polyphenols and pigments upon seasonal and habitat variations |
| topic | nettle leaves and stalks phenological stage location accelerated solvent extraction UPLC-MS/MS polyphenols |
| url | https://www.mdpi.com/2304-8158/10/1/190 |
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