The vitamin D analogue paricalcitol attenuates hepatic ischemia/reperfusion injury through down-regulation of Toll-like receptor 4 signaling in rats
Introduction : Recent studies have revealed that vitamin D and its synthetic analogues have a protective effect on experimental ischemia/reperfusion (I/R) models in several organs, but little is known about its effect on the liver. The aim of this study was to evaluate the beneficial effects of vita...
| Main Authors: | , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Termedia Publishing House
2016-06-01
|
| Series: | Archives of Medical Science |
| Subjects: | |
| Online Access: | https://www.termedia.pl/The-vitamin-D-analogue-paricalcitol-attenuates-hepatic-ischemia-reperfusion-injury-through-down-regulation-of-Toll-like-receptor-4-signaling-in-rats,19,27790,1,1.html |
| _version_ | 1819089989380603904 |
|---|---|
| author | Min Sung Kim Soyoung Lee Namhee Jung Kiho Lee Jinwoo Choi Sang-Hoon Kim Jinhyun Jun Won-Mee Lee Yeonsoo Chang Donghee Kim |
| author_facet | Min Sung Kim Soyoung Lee Namhee Jung Kiho Lee Jinwoo Choi Sang-Hoon Kim Jinhyun Jun Won-Mee Lee Yeonsoo Chang Donghee Kim |
| author_sort | Min Sung Kim |
| collection | DOAJ |
| description | Introduction : Recent studies have revealed that vitamin D and its synthetic analogues have a protective effect on experimental ischemia/reperfusion (I/R) models in several organs, but little is known about its effect on the liver. The aim of this study was to evaluate the beneficial effects of vitamin D in a model of liver I/R in rats, focusing on Toll-like receptor (TLR) 4 signaling, which has been shown to be involved in I/R injury.
Material and methods : Twenty-four male Wistar rats were randomized into four groups: Saline + Sham, Saline + I/R, Paricalcitol + Sham, and Paricalcitol + I/R. A synthetic vitamin D2 analogue, paricalcitol, was intraperitoneally injected 24 h prior to surgery. The animals were subjected to 60 min of partial warm ischemia (70%), followed by reperfusion for 6 h on the same day. The ischemic lobe of the liver and blood were collected for molecular biochemical analyses.
Results: Liver damage following I/R was diminished by pretreatment with paricalcitol. Pretreatment with paricalcitol decreased the levels of pro-inflammatory mediators, such as interleukin (IL)-1, tumor necrosis factor (TNF)-, and macrophage migration inhibitory factor (MIF), in both plasma and liver tissue. In addition, pretreatment with paricalcitol markedly down-regulated the expression of TLR4, HMGB1, TNF- and NF-B.
Conclusions : The vitamin D analogue paricalcitol attenuates hepatic I/R injury through down-regulation of the TLR4 signaling pathway and might be considered to be a potential nutritional therapeutic agent against I/R injury in the liver. |
| first_indexed | 2024-12-21T22:16:42Z |
| format | Article |
| id | doaj.art-bb126a89e60b4b2aa100c17c7c836c15 |
| institution | Directory Open Access Journal |
| issn | 1734-1922 1896-9151 |
| language | English |
| last_indexed | 2024-12-21T22:16:42Z |
| publishDate | 2016-06-01 |
| publisher | Termedia Publishing House |
| record_format | Article |
| series | Archives of Medical Science |
| spelling | doaj.art-bb126a89e60b4b2aa100c17c7c836c152022-12-21T18:48:27ZengTermedia Publishing HouseArchives of Medical Science1734-19221896-91512016-06-0113245946910.5114/aoms.2016.6065027790The vitamin D analogue paricalcitol attenuates hepatic ischemia/reperfusion injury through down-regulation of Toll-like receptor 4 signaling in ratsMin Sung KimSoyoung LeeNamhee JungKiho LeeJinwoo ChoiSang-Hoon KimJinhyun JunWon-Mee LeeYeonsoo ChangDonghee KimIntroduction : Recent studies have revealed that vitamin D and its synthetic analogues have a protective effect on experimental ischemia/reperfusion (I/R) models in several organs, but little is known about its effect on the liver. The aim of this study was to evaluate the beneficial effects of vitamin D in a model of liver I/R in rats, focusing on Toll-like receptor (TLR) 4 signaling, which has been shown to be involved in I/R injury. Material and methods : Twenty-four male Wistar rats were randomized into four groups: Saline + Sham, Saline + I/R, Paricalcitol + Sham, and Paricalcitol + I/R. A synthetic vitamin D2 analogue, paricalcitol, was intraperitoneally injected 24 h prior to surgery. The animals were subjected to 60 min of partial warm ischemia (70%), followed by reperfusion for 6 h on the same day. The ischemic lobe of the liver and blood were collected for molecular biochemical analyses. Results: Liver damage following I/R was diminished by pretreatment with paricalcitol. Pretreatment with paricalcitol decreased the levels of pro-inflammatory mediators, such as interleukin (IL)-1, tumor necrosis factor (TNF)-, and macrophage migration inhibitory factor (MIF), in both plasma and liver tissue. In addition, pretreatment with paricalcitol markedly down-regulated the expression of TLR4, HMGB1, TNF- and NF-B. Conclusions : The vitamin D analogue paricalcitol attenuates hepatic I/R injury through down-regulation of the TLR4 signaling pathway and might be considered to be a potential nutritional therapeutic agent against I/R injury in the liver.https://www.termedia.pl/The-vitamin-D-analogue-paricalcitol-attenuates-hepatic-ischemia-reperfusion-injury-through-down-regulation-of-Toll-like-receptor-4-signaling-in-rats,19,27790,1,1.htmlhepatic ischemia/reperfusion injury Toll-like receptor 4 vitamin D |
| spellingShingle | Min Sung Kim Soyoung Lee Namhee Jung Kiho Lee Jinwoo Choi Sang-Hoon Kim Jinhyun Jun Won-Mee Lee Yeonsoo Chang Donghee Kim The vitamin D analogue paricalcitol attenuates hepatic ischemia/reperfusion injury through down-regulation of Toll-like receptor 4 signaling in rats Archives of Medical Science hepatic ischemia/reperfusion injury Toll-like receptor 4 vitamin D |
| title | The vitamin D analogue paricalcitol attenuates hepatic ischemia/reperfusion injury through down-regulation of Toll-like receptor 4 signaling in rats |
| title_full | The vitamin D analogue paricalcitol attenuates hepatic ischemia/reperfusion injury through down-regulation of Toll-like receptor 4 signaling in rats |
| title_fullStr | The vitamin D analogue paricalcitol attenuates hepatic ischemia/reperfusion injury through down-regulation of Toll-like receptor 4 signaling in rats |
| title_full_unstemmed | The vitamin D analogue paricalcitol attenuates hepatic ischemia/reperfusion injury through down-regulation of Toll-like receptor 4 signaling in rats |
| title_short | The vitamin D analogue paricalcitol attenuates hepatic ischemia/reperfusion injury through down-regulation of Toll-like receptor 4 signaling in rats |
| title_sort | vitamin d analogue paricalcitol attenuates hepatic ischemia reperfusion injury through down regulation of toll like receptor 4 signaling in rats |
| topic | hepatic ischemia/reperfusion injury Toll-like receptor 4 vitamin D |
| url | https://www.termedia.pl/The-vitamin-D-analogue-paricalcitol-attenuates-hepatic-ischemia-reperfusion-injury-through-down-regulation-of-Toll-like-receptor-4-signaling-in-rats,19,27790,1,1.html |
| work_keys_str_mv | AT minsungkim thevitamindanalogueparicalcitolattenuateshepaticischemiareperfusioninjurythroughdownregulationoftolllikereceptor4signalinginrats AT soyounglee thevitamindanalogueparicalcitolattenuateshepaticischemiareperfusioninjurythroughdownregulationoftolllikereceptor4signalinginrats AT namheejung thevitamindanalogueparicalcitolattenuateshepaticischemiareperfusioninjurythroughdownregulationoftolllikereceptor4signalinginrats AT kiholee thevitamindanalogueparicalcitolattenuateshepaticischemiareperfusioninjurythroughdownregulationoftolllikereceptor4signalinginrats AT jinwoochoi thevitamindanalogueparicalcitolattenuateshepaticischemiareperfusioninjurythroughdownregulationoftolllikereceptor4signalinginrats AT sanghoonkim thevitamindanalogueparicalcitolattenuateshepaticischemiareperfusioninjurythroughdownregulationoftolllikereceptor4signalinginrats AT jinhyunjun thevitamindanalogueparicalcitolattenuateshepaticischemiareperfusioninjurythroughdownregulationoftolllikereceptor4signalinginrats AT wonmeelee thevitamindanalogueparicalcitolattenuateshepaticischemiareperfusioninjurythroughdownregulationoftolllikereceptor4signalinginrats AT yeonsoochang thevitamindanalogueparicalcitolattenuateshepaticischemiareperfusioninjurythroughdownregulationoftolllikereceptor4signalinginrats AT dongheekim thevitamindanalogueparicalcitolattenuateshepaticischemiareperfusioninjurythroughdownregulationoftolllikereceptor4signalinginrats AT minsungkim vitamindanalogueparicalcitolattenuateshepaticischemiareperfusioninjurythroughdownregulationoftolllikereceptor4signalinginrats AT soyounglee vitamindanalogueparicalcitolattenuateshepaticischemiareperfusioninjurythroughdownregulationoftolllikereceptor4signalinginrats AT namheejung vitamindanalogueparicalcitolattenuateshepaticischemiareperfusioninjurythroughdownregulationoftolllikereceptor4signalinginrats AT kiholee vitamindanalogueparicalcitolattenuateshepaticischemiareperfusioninjurythroughdownregulationoftolllikereceptor4signalinginrats AT jinwoochoi vitamindanalogueparicalcitolattenuateshepaticischemiareperfusioninjurythroughdownregulationoftolllikereceptor4signalinginrats AT sanghoonkim vitamindanalogueparicalcitolattenuateshepaticischemiareperfusioninjurythroughdownregulationoftolllikereceptor4signalinginrats AT jinhyunjun vitamindanalogueparicalcitolattenuateshepaticischemiareperfusioninjurythroughdownregulationoftolllikereceptor4signalinginrats AT wonmeelee vitamindanalogueparicalcitolattenuateshepaticischemiareperfusioninjurythroughdownregulationoftolllikereceptor4signalinginrats AT yeonsoochang vitamindanalogueparicalcitolattenuateshepaticischemiareperfusioninjurythroughdownregulationoftolllikereceptor4signalinginrats AT dongheekim vitamindanalogueparicalcitolattenuateshepaticischemiareperfusioninjurythroughdownregulationoftolllikereceptor4signalinginrats |