Inhibitory Effects and Killing Kinetics of Lactic Acid Rice Gel Against Pathogenic Bacteria Causing Bovine Mastitis
Staphylococcus aureus and S. epidermidis are the major teat skin bacteria and lead to severe bovine mastitis. Teat antiseptic is an important tool for controlling intramammary infection. The antibacterial activity of lactic acid (LA) against one reference strain of S. aureus ATCC 25923 and two field...
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MDPI AG
2018-07-01
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| Series: | Scientia Pharmaceutica |
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| Online Access: | http://www.mdpi.com/2218-0532/86/3/29 |
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| author | Rinrada Chotigarpa Kannika Na Lampang Surachai Pikulkaew Siriporn Okonogi Kittisak Ajariyakhajorn Raktham Mektrirat |
| author_facet | Rinrada Chotigarpa Kannika Na Lampang Surachai Pikulkaew Siriporn Okonogi Kittisak Ajariyakhajorn Raktham Mektrirat |
| author_sort | Rinrada Chotigarpa |
| collection | DOAJ |
| description | Staphylococcus aureus and S. epidermidis are the major teat skin bacteria and lead to severe bovine mastitis. Teat antiseptic is an important tool for controlling intramammary infection. The antibacterial activity of lactic acid (LA) against one reference strain of S. aureus ATCC 25923 and two field strains including S. aureus and S. epidermidis was investigated using the broth microdilution method. Its minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) were 0.5% for strains belonging to both species. An antiseptic preparation containing 5% LA with modified rice gel (LA-RG) was successfully prepared. Rheological behavior of LA-RG was found to be a pseudoplastic flow with thixotropy with viscosity of approximately 0.007 Pas. LA-RG exhibited a sufficient adhesive property in the rolling ball test with a length of 9.67 ± 0.04 cm. Killing kinetic studies of LA-RG showed that the killing rate of LA-RG was significantly faster than that of LA. After 32 min of exposure to LA-RG, approximately 86% and 60% of S. aureus and S. epidermidis were reduced, respectively. Abnormal bacterial cell surface after exposure to LA-RG was observed by scanning electron microscopy. It is concluded that LA-RG is a promising preparation as an alternative product for preventing mastitis in dairy cattle. |
| first_indexed | 2024-04-11T22:24:01Z |
| format | Article |
| id | doaj.art-bb149d18aecb4fbf8b2256c3f36b4748 |
| institution | Directory Open Access Journal |
| issn | 2218-0532 |
| language | English |
| last_indexed | 2024-04-11T22:24:01Z |
| publishDate | 2018-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Scientia Pharmaceutica |
| spelling | doaj.art-bb149d18aecb4fbf8b2256c3f36b47482022-12-22T03:59:56ZengMDPI AGScientia Pharmaceutica2218-05322018-07-018632910.3390/scipharm86030029scipharm86030029Inhibitory Effects and Killing Kinetics of Lactic Acid Rice Gel Against Pathogenic Bacteria Causing Bovine MastitisRinrada Chotigarpa0Kannika Na Lampang1Surachai Pikulkaew2Siriporn Okonogi3Kittisak Ajariyakhajorn4Raktham Mektrirat5Department of Veterinary Biosciences and Public Health, Faculty of Veterinary Medicine, Chiang Mai University, Chiang Mai 50100, ThailandDepartment of Veterinary Biosciences and Public Health, Faculty of Veterinary Medicine, Chiang Mai University, Chiang Mai 50100, ThailandDepartment of Food Animal Clinic, Faculty of Veterinary Medicine, Chiang Mai University, Chiang Mai 50100, ThailandResearch Center of Pharmaceutical Nanotechnology, Chiang Mai University, Chiang Mai 50200, ThailandDepartment of Veterinary Medicine, Faculty of Veterinary Science, Chulalongkorn University, Bangkok 10330, ThailandDepartment of Veterinary Biosciences and Public Health, Faculty of Veterinary Medicine, Chiang Mai University, Chiang Mai 50100, ThailandStaphylococcus aureus and S. epidermidis are the major teat skin bacteria and lead to severe bovine mastitis. Teat antiseptic is an important tool for controlling intramammary infection. The antibacterial activity of lactic acid (LA) against one reference strain of S. aureus ATCC 25923 and two field strains including S. aureus and S. epidermidis was investigated using the broth microdilution method. Its minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) were 0.5% for strains belonging to both species. An antiseptic preparation containing 5% LA with modified rice gel (LA-RG) was successfully prepared. Rheological behavior of LA-RG was found to be a pseudoplastic flow with thixotropy with viscosity of approximately 0.007 Pas. LA-RG exhibited a sufficient adhesive property in the rolling ball test with a length of 9.67 ± 0.04 cm. Killing kinetic studies of LA-RG showed that the killing rate of LA-RG was significantly faster than that of LA. After 32 min of exposure to LA-RG, approximately 86% and 60% of S. aureus and S. epidermidis were reduced, respectively. Abnormal bacterial cell surface after exposure to LA-RG was observed by scanning electron microscopy. It is concluded that LA-RG is a promising preparation as an alternative product for preventing mastitis in dairy cattle.http://www.mdpi.com/2218-0532/86/3/29riceantisepticteat skinlactic acid |
| spellingShingle | Rinrada Chotigarpa Kannika Na Lampang Surachai Pikulkaew Siriporn Okonogi Kittisak Ajariyakhajorn Raktham Mektrirat Inhibitory Effects and Killing Kinetics of Lactic Acid Rice Gel Against Pathogenic Bacteria Causing Bovine Mastitis Scientia Pharmaceutica rice antiseptic teat skin lactic acid |
| title | Inhibitory Effects and Killing Kinetics of Lactic Acid Rice Gel Against Pathogenic Bacteria Causing Bovine Mastitis |
| title_full | Inhibitory Effects and Killing Kinetics of Lactic Acid Rice Gel Against Pathogenic Bacteria Causing Bovine Mastitis |
| title_fullStr | Inhibitory Effects and Killing Kinetics of Lactic Acid Rice Gel Against Pathogenic Bacteria Causing Bovine Mastitis |
| title_full_unstemmed | Inhibitory Effects and Killing Kinetics of Lactic Acid Rice Gel Against Pathogenic Bacteria Causing Bovine Mastitis |
| title_short | Inhibitory Effects and Killing Kinetics of Lactic Acid Rice Gel Against Pathogenic Bacteria Causing Bovine Mastitis |
| title_sort | inhibitory effects and killing kinetics of lactic acid rice gel against pathogenic bacteria causing bovine mastitis |
| topic | rice antiseptic teat skin lactic acid |
| url | http://www.mdpi.com/2218-0532/86/3/29 |
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