Surface Assembly of DNA Origami on a Lipid Bilayer Observed Using High-Speed Atomic Force Microscopy
The micrometer-scale assembly of various DNA nanostructures is one of the major challenges for further progress in DNA nanotechnology. Programmed patterns of 1D and 2D DNA origami assembly using specific DNA strands and micrometer-sized lattice assembly using cross-shaped DNA origami were performed...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-06-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/27/13/4224 |
| _version_ | 1797408603094646784 |
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| author | Masayuki Endo |
| author_facet | Masayuki Endo |
| author_sort | Masayuki Endo |
| collection | DOAJ |
| description | The micrometer-scale assembly of various DNA nanostructures is one of the major challenges for further progress in DNA nanotechnology. Programmed patterns of 1D and 2D DNA origami assembly using specific DNA strands and micrometer-sized lattice assembly using cross-shaped DNA origami were performed on a lipid bilayer surface. During the diffusion of DNA origami on the membrane surface, the formation of lattices and their rearrangement in real-time were observed using high-speed atomic force microscopy (HS-AFM). The formed lattices were used to further assemble DNA origami tiles into their cavities. Various patterns of lattice–tile complexes were created by changing the interactions between the lattice and tiles. For the control of the nanostructure formation, the photo-controlled assembly and disassembly of DNA origami were performed reversibly, and dynamic assembly and disassembly were observed on a lipid bilayer surface using HS-AFM. Using a lipid bilayer for DNA origami assembly, it is possible to perform a hierarchical assembly of multiple DNA origami nanostructures, such as the integration of functional components into a frame architecture. |
| first_indexed | 2024-03-09T04:00:45Z |
| format | Article |
| id | doaj.art-bb15f8e011d845e99c63c3eba81fbb5a |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-09T04:00:45Z |
| publishDate | 2022-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-bb15f8e011d845e99c63c3eba81fbb5a2023-12-03T14:13:56ZengMDPI AGMolecules1420-30492022-06-012713422410.3390/molecules27134224Surface Assembly of DNA Origami on a Lipid Bilayer Observed Using High-Speed Atomic Force MicroscopyMasayuki Endo0Organization for Research and Development of Innovative Science and Technology, Kansai University, Suita, Osaka 564-8680, JapanThe micrometer-scale assembly of various DNA nanostructures is one of the major challenges for further progress in DNA nanotechnology. Programmed patterns of 1D and 2D DNA origami assembly using specific DNA strands and micrometer-sized lattice assembly using cross-shaped DNA origami were performed on a lipid bilayer surface. During the diffusion of DNA origami on the membrane surface, the formation of lattices and their rearrangement in real-time were observed using high-speed atomic force microscopy (HS-AFM). The formed lattices were used to further assemble DNA origami tiles into their cavities. Various patterns of lattice–tile complexes were created by changing the interactions between the lattice and tiles. For the control of the nanostructure formation, the photo-controlled assembly and disassembly of DNA origami were performed reversibly, and dynamic assembly and disassembly were observed on a lipid bilayer surface using HS-AFM. Using a lipid bilayer for DNA origami assembly, it is possible to perform a hierarchical assembly of multiple DNA origami nanostructures, such as the integration of functional components into a frame architecture.https://www.mdpi.com/1420-3049/27/13/4224DNA origamiDNA nanotechnologysurface assemblylipid bilayerhigh-speed AFM |
| spellingShingle | Masayuki Endo Surface Assembly of DNA Origami on a Lipid Bilayer Observed Using High-Speed Atomic Force Microscopy Molecules DNA origami DNA nanotechnology surface assembly lipid bilayer high-speed AFM |
| title | Surface Assembly of DNA Origami on a Lipid Bilayer Observed Using High-Speed Atomic Force Microscopy |
| title_full | Surface Assembly of DNA Origami on a Lipid Bilayer Observed Using High-Speed Atomic Force Microscopy |
| title_fullStr | Surface Assembly of DNA Origami on a Lipid Bilayer Observed Using High-Speed Atomic Force Microscopy |
| title_full_unstemmed | Surface Assembly of DNA Origami on a Lipid Bilayer Observed Using High-Speed Atomic Force Microscopy |
| title_short | Surface Assembly of DNA Origami on a Lipid Bilayer Observed Using High-Speed Atomic Force Microscopy |
| title_sort | surface assembly of dna origami on a lipid bilayer observed using high speed atomic force microscopy |
| topic | DNA origami DNA nanotechnology surface assembly lipid bilayer high-speed AFM |
| url | https://www.mdpi.com/1420-3049/27/13/4224 |
| work_keys_str_mv | AT masayukiendo surfaceassemblyofdnaorigamionalipidbilayerobservedusinghighspeedatomicforcemicroscopy |