How Do Phenolic Acids Change the Secondary and Tertiary Structure of Gliadin? Studies with an Application of Spectroscopic Techniques
The effect of the chemical structure of selected phenolic acids on the molecular organization of gliadins was investigated with the application of Fourier Transform Infrared (FTIR) technique, steady-state, and time-resolved fluorescence spectroscopy. Hydroxybenzoic (4-hydroxybenzoic, protocatechuic,...
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| Format: | Article |
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MDPI AG
2022-05-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/23/11/6053 |
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| author | Renata Welc Rafał Luchowski Konrad Kłosok Wiesław I. Gruszecki Agnieszka Nawrocka |
| author_facet | Renata Welc Rafał Luchowski Konrad Kłosok Wiesław I. Gruszecki Agnieszka Nawrocka |
| author_sort | Renata Welc |
| collection | DOAJ |
| description | The effect of the chemical structure of selected phenolic acids on the molecular organization of gliadins was investigated with the application of Fourier Transform Infrared (FTIR) technique, steady-state, and time-resolved fluorescence spectroscopy. Hydroxybenzoic (4-hydroxybenzoic, protocatechuic, vanillic, and syringic) and hydroxycinnamic (coumaric, caffeic, ferulic, sinapic) acids have been used as gliadins modifiers. The results indicated that hydroxybenzoic acids due to their smaller size incorporate into spaces between two polypeptide chains and form a hydrogen bond with them leading to aggregation. Additionally, syringic acids could incorporate into hydrophobic pockets of protein. Whereas hydroxycinnamic acids, due to their higher stiffness and larger size, separated polypeptide chains leading to gliadin disaggregation. These acids did not incorporate into hydrophobic pockets. |
| first_indexed | 2024-03-10T01:16:25Z |
| format | Article |
| id | doaj.art-bb165a168cab41b0ae8a6cec555dad6f |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-10T01:16:25Z |
| publishDate | 2022-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-bb165a168cab41b0ae8a6cec555dad6f2023-11-23T14:08:45ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672022-05-012311605310.3390/ijms23116053How Do Phenolic Acids Change the Secondary and Tertiary Structure of Gliadin? Studies with an Application of Spectroscopic TechniquesRenata Welc0Rafał Luchowski1Konrad Kłosok2Wiesław I. Gruszecki3Agnieszka Nawrocka4Institute of Agrophysics, Polish Academy of Sciences, Doświadczalna 4, 20-290 Lublin, PolandDepartment of Biophysics, Institute of Physics, Maria Curie Skłodowska University, 20-031 Lublin, PolandInstitute of Agrophysics, Polish Academy of Sciences, Doświadczalna 4, 20-290 Lublin, PolandDepartment of Biophysics, Institute of Physics, Maria Curie Skłodowska University, 20-031 Lublin, PolandInstitute of Agrophysics, Polish Academy of Sciences, Doświadczalna 4, 20-290 Lublin, PolandThe effect of the chemical structure of selected phenolic acids on the molecular organization of gliadins was investigated with the application of Fourier Transform Infrared (FTIR) technique, steady-state, and time-resolved fluorescence spectroscopy. Hydroxybenzoic (4-hydroxybenzoic, protocatechuic, vanillic, and syringic) and hydroxycinnamic (coumaric, caffeic, ferulic, sinapic) acids have been used as gliadins modifiers. The results indicated that hydroxybenzoic acids due to their smaller size incorporate into spaces between two polypeptide chains and form a hydrogen bond with them leading to aggregation. Additionally, syringic acids could incorporate into hydrophobic pockets of protein. Whereas hydroxycinnamic acids, due to their higher stiffness and larger size, separated polypeptide chains leading to gliadin disaggregation. These acids did not incorporate into hydrophobic pockets.https://www.mdpi.com/1422-0067/23/11/6053gliadinphenolic acidssecondary structuretime-resolved fluorescenceFTIR technique |
| spellingShingle | Renata Welc Rafał Luchowski Konrad Kłosok Wiesław I. Gruszecki Agnieszka Nawrocka How Do Phenolic Acids Change the Secondary and Tertiary Structure of Gliadin? Studies with an Application of Spectroscopic Techniques International Journal of Molecular Sciences gliadin phenolic acids secondary structure time-resolved fluorescence FTIR technique |
| title | How Do Phenolic Acids Change the Secondary and Tertiary Structure of Gliadin? Studies with an Application of Spectroscopic Techniques |
| title_full | How Do Phenolic Acids Change the Secondary and Tertiary Structure of Gliadin? Studies with an Application of Spectroscopic Techniques |
| title_fullStr | How Do Phenolic Acids Change the Secondary and Tertiary Structure of Gliadin? Studies with an Application of Spectroscopic Techniques |
| title_full_unstemmed | How Do Phenolic Acids Change the Secondary and Tertiary Structure of Gliadin? Studies with an Application of Spectroscopic Techniques |
| title_short | How Do Phenolic Acids Change the Secondary and Tertiary Structure of Gliadin? Studies with an Application of Spectroscopic Techniques |
| title_sort | how do phenolic acids change the secondary and tertiary structure of gliadin studies with an application of spectroscopic techniques |
| topic | gliadin phenolic acids secondary structure time-resolved fluorescence FTIR technique |
| url | https://www.mdpi.com/1422-0067/23/11/6053 |
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