In Vitro Inhibition of Human Sperm Creatine Kinase by Nicotine, Cotinine and Cadmium, as a Mechanism in Smoker Men Infertility
Background Nicotine, cotinine and cadmium are harmful components of cigarettes that have an effect on human reproductive function. Although the effects of cigarette smoke on male reproductive function is characterized in several articles its mechanism of action is still unknown. In the present study...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
| Published: |
Royan Institute (ACECR), Tehran
2008-11-01
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| Series: | International Journal of Fertility and Sterility |
| Subjects: | |
| Online Access: | http://www.ijfs.ir/article_45724_c3170027b2577b696d9b215837d7aa1b.pdf |
| _version_ | 1819007753006350336 |
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| author | Mohammad Ali Ghaffari Mohammad Abromand Behrooz Motlagh |
| author_facet | Mohammad Ali Ghaffari Mohammad Abromand Behrooz Motlagh |
| author_sort | Mohammad Ali Ghaffari |
| collection | DOAJ |
| description | Background
Nicotine, cotinine and cadmium are harmful components of cigarettes that have an effect on human reproductive function. Although the effects of cigarette smoke on male reproductive function is characterized in several articles its mechanism of action is still unknown. In the present study, we investigate the effect of nicotine, cotinine and cadmium on human sperm creatine kinase activity in vitro.
Materials and methods
Total creatine kinase activity is measured in sperm homogenates after chromatography on a diethylaminoethyl cellulose (DEAE-32) column.
Results
We show that creatine kinase activity is significantly inhibited by nicotine (44%), cotinine (39%) and cadmium (65%) at a concentration of 60 μg/ml. Kinetic studies reveal that the inhibitory effect of nicotine, cotinine and cadmium are competitive in relation to creatine phosphate.
Conclusion
Considering the importance of creatine kinase activity for normal sperm energy metabolism, our results suggest that inhibition of this enzyme by nicotine, cotinine and cadmium may be an important mechanism in infertility amongst male smokers. However, further investigations are needed to elucidate the exact mechanism of cigarette effect on male reproductive function at the molecular level. |
| first_indexed | 2024-12-21T00:29:35Z |
| format | Article |
| id | doaj.art-bb178332b14540b9abb2dcb0367f71a3 |
| institution | Directory Open Access Journal |
| issn | 2008-076X 2008-0778 |
| language | English |
| last_indexed | 2024-12-21T00:29:35Z |
| publishDate | 2008-11-01 |
| publisher | Royan Institute (ACECR), Tehran |
| record_format | Article |
| series | International Journal of Fertility and Sterility |
| spelling | doaj.art-bb178332b14540b9abb2dcb0367f71a32022-12-21T19:21:55ZengRoyan Institute (ACECR), TehranInternational Journal of Fertility and Sterility2008-076X2008-07782008-11-012312513010.22074/ijfs.2008.4572445724In Vitro Inhibition of Human Sperm Creatine Kinase by Nicotine, Cotinine and Cadmium, as a Mechanism in Smoker Men InfertilityMohammad Ali Ghaffari0Mohammad Abromand1Behrooz Motlagh2Biochemistry Department, Faculty of Medicine, Ahwaz Jundishapour University of Medical Sciences, Ahwaz, IranBiochemistry Department, Faculty of Medicine, Ahwaz Jundishapour University of Medical Sciences, Ahwaz, IranBiochemistry Department, Faculty of Medicine, Ahwaz Jundishapour University of Medical Sciences, Ahwaz, IranBackground Nicotine, cotinine and cadmium are harmful components of cigarettes that have an effect on human reproductive function. Although the effects of cigarette smoke on male reproductive function is characterized in several articles its mechanism of action is still unknown. In the present study, we investigate the effect of nicotine, cotinine and cadmium on human sperm creatine kinase activity in vitro. Materials and methods Total creatine kinase activity is measured in sperm homogenates after chromatography on a diethylaminoethyl cellulose (DEAE-32) column. Results We show that creatine kinase activity is significantly inhibited by nicotine (44%), cotinine (39%) and cadmium (65%) at a concentration of 60 μg/ml. Kinetic studies reveal that the inhibitory effect of nicotine, cotinine and cadmium are competitive in relation to creatine phosphate. Conclusion Considering the importance of creatine kinase activity for normal sperm energy metabolism, our results suggest that inhibition of this enzyme by nicotine, cotinine and cadmium may be an important mechanism in infertility amongst male smokers. However, further investigations are needed to elucidate the exact mechanism of cigarette effect on male reproductive function at the molecular level.http://www.ijfs.ir/article_45724_c3170027b2577b696d9b215837d7aa1b.pdfspermcreatine kinasenicotinecotininecadmium |
| spellingShingle | Mohammad Ali Ghaffari Mohammad Abromand Behrooz Motlagh In Vitro Inhibition of Human Sperm Creatine Kinase by Nicotine, Cotinine and Cadmium, as a Mechanism in Smoker Men Infertility International Journal of Fertility and Sterility sperm creatine kinase nicotine cotinine cadmium |
| title | In Vitro Inhibition of Human Sperm Creatine Kinase by Nicotine, Cotinine and Cadmium, as a Mechanism in Smoker Men Infertility |
| title_full | In Vitro Inhibition of Human Sperm Creatine Kinase by Nicotine, Cotinine and Cadmium, as a Mechanism in Smoker Men Infertility |
| title_fullStr | In Vitro Inhibition of Human Sperm Creatine Kinase by Nicotine, Cotinine and Cadmium, as a Mechanism in Smoker Men Infertility |
| title_full_unstemmed | In Vitro Inhibition of Human Sperm Creatine Kinase by Nicotine, Cotinine and Cadmium, as a Mechanism in Smoker Men Infertility |
| title_short | In Vitro Inhibition of Human Sperm Creatine Kinase by Nicotine, Cotinine and Cadmium, as a Mechanism in Smoker Men Infertility |
| title_sort | in vitro inhibition of human sperm creatine kinase by nicotine cotinine and cadmium as a mechanism in smoker men infertility |
| topic | sperm creatine kinase nicotine cotinine cadmium |
| url | http://www.ijfs.ir/article_45724_c3170027b2577b696d9b215837d7aa1b.pdf |
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