The effect of thioredoxin‐1 in a rat model of traumatic brain injury depending on diurnal variation
Abstract Introduction Traumatic brain injury (TBI) is a public health concern with limited treatment options because it causes a cascade of side effects that are the leading cause of hospital death. Thioredoxin is an enzyme with neuroprotective properties such as antioxidant, antiapoptotic, immune r...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Wiley
2023-06-01
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| Series: | Brain and Behavior |
| Subjects: | |
| Online Access: | https://doi.org/10.1002/brb3.3031 |
| _version_ | 1797802401418182656 |
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| author | Roxana Noriega‐Navarro Ricardo Jesús Martínez‐Tapia Rubén González‐Rivera Alicia Ochoa‐Sánchez Julio César Abarca‐Magaña Lucía Landa‐Navarro Verónica Rodríguez‐Mata Perla Ugalde‐Muñiz Armando Pérez‐Torres Abraham Landa Luz Navarro |
| author_facet | Roxana Noriega‐Navarro Ricardo Jesús Martínez‐Tapia Rubén González‐Rivera Alicia Ochoa‐Sánchez Julio César Abarca‐Magaña Lucía Landa‐Navarro Verónica Rodríguez‐Mata Perla Ugalde‐Muñiz Armando Pérez‐Torres Abraham Landa Luz Navarro |
| author_sort | Roxana Noriega‐Navarro |
| collection | DOAJ |
| description | Abstract Introduction Traumatic brain injury (TBI) is a public health concern with limited treatment options because it causes a cascade of side effects that are the leading cause of hospital death. Thioredoxin is an enzyme with neuroprotective properties such as antioxidant, antiapoptotic, immune response modulator, and neurogenic, among others; it has been considered a therapeutic target for treating many disorders. Methods The controlled cortical impact (CCI) model was used to assess the effect of recombinant human thioredoxin 1 (rhTrx1) (1 μg/2 μL, intracortical) on rats subjected to TBI at two different times of the light‐dark cycle (01:00 and 13:00 h). We analyzed the food intake, body weight loss, motor coordination, pain perception, and histology in specific hippocampus (CA1, CA2, CA3, and Dental Gyrus) and striatum (caudate‐putamen) areas. Results Body weight loss, reduced food intake, spontaneous pain, motor impairment, and neuronal damage in specific hippocampus and striatum regions are more evident in rats subjected to TBI in the light phase than in the dark phase of the cycle and in groups that did not receive rhTrx1 or minocycline (as positive control). Three days after TBI, there is a recovery in body weight, food intake, motor impairment, and pain, which is more pronounced in the rats subjected to TBI at the dark phase of the cycle and those that received rhTrx1 or minocycline. Conclusions Knowing the time of day a TBI occurs in connection to the neuroprotective mechanisms of the immune response in diurnal variation and the usage of the Trx1 protein might have a beneficial therapeutic impact in promoting quick recovery after a TBI. |
| first_indexed | 2024-03-13T05:05:08Z |
| format | Article |
| id | doaj.art-bb1927bcc15c4930b7d5ec94a5a59b5b |
| institution | Directory Open Access Journal |
| issn | 2162-3279 |
| language | English |
| last_indexed | 2024-03-13T05:05:08Z |
| publishDate | 2023-06-01 |
| publisher | Wiley |
| record_format | Article |
| series | Brain and Behavior |
| spelling | doaj.art-bb1927bcc15c4930b7d5ec94a5a59b5b2023-06-16T18:11:55ZengWileyBrain and Behavior2162-32792023-06-01136n/an/a10.1002/brb3.3031The effect of thioredoxin‐1 in a rat model of traumatic brain injury depending on diurnal variationRoxana Noriega‐Navarro0Ricardo Jesús Martínez‐Tapia1Rubén González‐Rivera2Alicia Ochoa‐Sánchez3Julio César Abarca‐Magaña4Lucía Landa‐Navarro5Verónica Rodríguez‐Mata6Perla Ugalde‐Muñiz7Armando Pérez‐Torres8Abraham Landa9Luz Navarro10Departamento de Fisiología, Facultad de Medicina Universidad Nacional Autónoma de México Ciudad de México MéxicoDepartamento de Fisiología, Facultad de Medicina Universidad Nacional Autónoma de México Ciudad de México MéxicoDepartamento de Fisiología, Facultad de Medicina Universidad Nacional Autónoma de México Ciudad de México MéxicoDepartamento de Microbiología y Parasitología, Facultad de Medicina Universidad Nacional Autónoma de México Ciudad de México MéxicoDepartamento de Fisiología, Facultad de Medicina Universidad Nacional Autónoma de México Ciudad de México MéxicoSimons Initiative for the Developing Brain, Centre for Discovery Brain Sciences University of Edinburgh Edinburgh UKDepartamento de Biología Celular y Tisular, Facultad de Medicina Universidad Nacional Autónoma de México Ciudad de México MéxicoDepartamento de Fisiología, Facultad de Medicina Universidad Nacional Autónoma de México Ciudad de México MéxicoDepartamento de Biología Celular y Tisular, Facultad de Medicina Universidad Nacional Autónoma de México Ciudad de México MéxicoDepartamento de Microbiología y Parasitología, Facultad de Medicina Universidad Nacional Autónoma de México Ciudad de México MéxicoDepartamento de Fisiología, Facultad de Medicina Universidad Nacional Autónoma de México Ciudad de México MéxicoAbstract Introduction Traumatic brain injury (TBI) is a public health concern with limited treatment options because it causes a cascade of side effects that are the leading cause of hospital death. Thioredoxin is an enzyme with neuroprotective properties such as antioxidant, antiapoptotic, immune response modulator, and neurogenic, among others; it has been considered a therapeutic target for treating many disorders. Methods The controlled cortical impact (CCI) model was used to assess the effect of recombinant human thioredoxin 1 (rhTrx1) (1 μg/2 μL, intracortical) on rats subjected to TBI at two different times of the light‐dark cycle (01:00 and 13:00 h). We analyzed the food intake, body weight loss, motor coordination, pain perception, and histology in specific hippocampus (CA1, CA2, CA3, and Dental Gyrus) and striatum (caudate‐putamen) areas. Results Body weight loss, reduced food intake, spontaneous pain, motor impairment, and neuronal damage in specific hippocampus and striatum regions are more evident in rats subjected to TBI in the light phase than in the dark phase of the cycle and in groups that did not receive rhTrx1 or minocycline (as positive control). Three days after TBI, there is a recovery in body weight, food intake, motor impairment, and pain, which is more pronounced in the rats subjected to TBI at the dark phase of the cycle and those that received rhTrx1 or minocycline. Conclusions Knowing the time of day a TBI occurs in connection to the neuroprotective mechanisms of the immune response in diurnal variation and the usage of the Trx1 protein might have a beneficial therapeutic impact in promoting quick recovery after a TBI.https://doi.org/10.1002/brb3.3031diurnal variationneuroprotectionthioredoxintraumatic brain injury |
| spellingShingle | Roxana Noriega‐Navarro Ricardo Jesús Martínez‐Tapia Rubén González‐Rivera Alicia Ochoa‐Sánchez Julio César Abarca‐Magaña Lucía Landa‐Navarro Verónica Rodríguez‐Mata Perla Ugalde‐Muñiz Armando Pérez‐Torres Abraham Landa Luz Navarro The effect of thioredoxin‐1 in a rat model of traumatic brain injury depending on diurnal variation Brain and Behavior diurnal variation neuroprotection thioredoxin traumatic brain injury |
| title | The effect of thioredoxin‐1 in a rat model of traumatic brain injury depending on diurnal variation |
| title_full | The effect of thioredoxin‐1 in a rat model of traumatic brain injury depending on diurnal variation |
| title_fullStr | The effect of thioredoxin‐1 in a rat model of traumatic brain injury depending on diurnal variation |
| title_full_unstemmed | The effect of thioredoxin‐1 in a rat model of traumatic brain injury depending on diurnal variation |
| title_short | The effect of thioredoxin‐1 in a rat model of traumatic brain injury depending on diurnal variation |
| title_sort | effect of thioredoxin 1 in a rat model of traumatic brain injury depending on diurnal variation |
| topic | diurnal variation neuroprotection thioredoxin traumatic brain injury |
| url | https://doi.org/10.1002/brb3.3031 |
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