Marine-Source Quorum Quenching Enzyme YtnP to Improve Hygiene Quality in Dental Units
Biofilm in dental unit water lines may pose a health risk to patients and dental practitioners. An AdiC-like quorum quenching enzyme, YtnP, was cloned from a deep-sea probiotic <i>Bacillus velezensis</i>, and heterologously expressed in <i>E. coli</i> to examine the applicati...
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-04-01
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| Series: | Marine Drugs |
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| Online Access: | https://www.mdpi.com/1660-3397/19/4/225 |
| _version_ | 1797537408151977984 |
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| author | Xiaohui Sun Philip Hill Jia Liu Jing Qian Yuting Ma Shufeng Zhou |
| author_facet | Xiaohui Sun Philip Hill Jia Liu Jing Qian Yuting Ma Shufeng Zhou |
| author_sort | Xiaohui Sun |
| collection | DOAJ |
| description | Biofilm in dental unit water lines may pose a health risk to patients and dental practitioners. An AdiC-like quorum quenching enzyme, YtnP, was cloned from a deep-sea probiotic <i>Bacillus velezensis</i>, and heterologously expressed in <i>E. coli</i> to examine the application on the improvement of hygiene problems caused by biofilm infection of <i>Pseudomonas aeruginosa</i> in dental units. <i>Pseudomonas</i> bacteria were isolated from dental chair units and used to grow static biofilms in the laboratory. A water filter system was designed to test the antifouling activity of YtnP in Laboratory, to simulate the biofilm contamination on water filter in dental unit water lines. The results demonstrated that the enzyme of YtnP was able to degrade the N-acyl homoserine lactones, significantly inhibited the EPS generation, biofilm formation, and virulence factors production (pyocyanin and rhamnolipid) of <i>P. aeruginosa</i>, and was efficient on the antifouling against <i>P. aeruginosa</i>. The findings in this study indicated the possibility of YtnP as novel disinfectant reagent for hygiene treatment in dental units. |
| first_indexed | 2024-03-10T12:15:43Z |
| format | Article |
| id | doaj.art-bb1df93fb7b8460cbc3451eb4932c722 |
| institution | Directory Open Access Journal |
| issn | 1660-3397 |
| language | English |
| last_indexed | 2024-03-10T12:15:43Z |
| publishDate | 2021-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Marine Drugs |
| spelling | doaj.art-bb1df93fb7b8460cbc3451eb4932c7222023-11-21T15:54:21ZengMDPI AGMarine Drugs1660-33972021-04-0119422510.3390/md19040225Marine-Source Quorum Quenching Enzyme YtnP to Improve Hygiene Quality in Dental UnitsXiaohui Sun0Philip Hill1Jia Liu2Jing Qian3Yuting Ma4Shufeng Zhou5College of Chemical Engineering, Huaqiao University, Xiamen 361021, ChinaSchool of Biosciences, Sutton Bonington Campus, University of Nottingham, Loughbrough LE12 5RD, UKCollege of Chemical Engineering, Huaqiao University, Xiamen 361021, ChinaCollege of Chemical Engineering, Huaqiao University, Xiamen 361021, ChinaCollege of Chemical Engineering, Huaqiao University, Xiamen 361021, ChinaCollege of Chemical Engineering, Huaqiao University, Xiamen 361021, ChinaBiofilm in dental unit water lines may pose a health risk to patients and dental practitioners. An AdiC-like quorum quenching enzyme, YtnP, was cloned from a deep-sea probiotic <i>Bacillus velezensis</i>, and heterologously expressed in <i>E. coli</i> to examine the application on the improvement of hygiene problems caused by biofilm infection of <i>Pseudomonas aeruginosa</i> in dental units. <i>Pseudomonas</i> bacteria were isolated from dental chair units and used to grow static biofilms in the laboratory. A water filter system was designed to test the antifouling activity of YtnP in Laboratory, to simulate the biofilm contamination on water filter in dental unit water lines. The results demonstrated that the enzyme of YtnP was able to degrade the N-acyl homoserine lactones, significantly inhibited the EPS generation, biofilm formation, and virulence factors production (pyocyanin and rhamnolipid) of <i>P. aeruginosa</i>, and was efficient on the antifouling against <i>P. aeruginosa</i>. The findings in this study indicated the possibility of YtnP as novel disinfectant reagent for hygiene treatment in dental units.https://www.mdpi.com/1660-3397/19/4/225quorum quenchingYtnPhygiene quality<i>Pseudomonas aeruginosa</i>oral infection |
| spellingShingle | Xiaohui Sun Philip Hill Jia Liu Jing Qian Yuting Ma Shufeng Zhou Marine-Source Quorum Quenching Enzyme YtnP to Improve Hygiene Quality in Dental Units Marine Drugs quorum quenching YtnP hygiene quality <i>Pseudomonas aeruginosa</i> oral infection |
| title | Marine-Source Quorum Quenching Enzyme YtnP to Improve Hygiene Quality in Dental Units |
| title_full | Marine-Source Quorum Quenching Enzyme YtnP to Improve Hygiene Quality in Dental Units |
| title_fullStr | Marine-Source Quorum Quenching Enzyme YtnP to Improve Hygiene Quality in Dental Units |
| title_full_unstemmed | Marine-Source Quorum Quenching Enzyme YtnP to Improve Hygiene Quality in Dental Units |
| title_short | Marine-Source Quorum Quenching Enzyme YtnP to Improve Hygiene Quality in Dental Units |
| title_sort | marine source quorum quenching enzyme ytnp to improve hygiene quality in dental units |
| topic | quorum quenching YtnP hygiene quality <i>Pseudomonas aeruginosa</i> oral infection |
| url | https://www.mdpi.com/1660-3397/19/4/225 |
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