Immobilization of Lipases on Chitosan Hydrogels Improves Their Stability in the Presence of the Products of Triglyceride Oxidation
A significant bottleneck for the industrial application of lipases stems from their poor stability in the presence of commercial triglycerides. This is mainly due to the inactivating effect of the products of triglyceride oxidation (PTO), which are usually produced when oils and fats, being imported...
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MDPI AG
2023-09-01
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Online Access: | https://www.mdpi.com/2310-2861/9/10/776 |
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author | Domenico Pirozzi Alessandro Latte Filomena Sannino |
author_facet | Domenico Pirozzi Alessandro Latte Filomena Sannino |
author_sort | Domenico Pirozzi |
collection | DOAJ |
description | A significant bottleneck for the industrial application of lipases stems from their poor stability in the presence of commercial triglycerides. This is mainly due to the inactivating effect of the products of triglyceride oxidation (PTO), which are usually produced when oils and fats, being imported from far countries, are stored for long periods. In this study, the immobilization of a lipase from <i>Candida rugosa</i> on chitosan hydrogels has been carried out following two alternative approaches based on the enzyme adsorption and entrapment to increase the lipase stability under the operating conditions that are typical of oleochemical transformations. The effect of model compounds representing different classes of PTO on a lipase has been studied to optimize the enzyme immobilization method. Particular attention has been devoted to the characterization of the inactivating effect of PTO in nonaqueous media, which are adopted for most industrial applications of lipases. |
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institution | Directory Open Access Journal |
issn | 2310-2861 |
language | English |
last_indexed | 2024-03-10T21:14:01Z |
publishDate | 2023-09-01 |
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spelling | doaj.art-bb21cf4004c247af81988a040d7d83862023-11-19T16:35:11ZengMDPI AGGels2310-28612023-09-0191077610.3390/gels9100776Immobilization of Lipases on Chitosan Hydrogels Improves Their Stability in the Presence of the Products of Triglyceride OxidationDomenico Pirozzi0Alessandro Latte1Filomena Sannino2Laboratory of Biochemical Engineering, Department of Chemical Engineering, Materials and Industrial Production (DICMaPI), University of Naples Federico II, Piazzale Tecchio 80, 80125 Naples, ItalyLaboratory of Biochemical Engineering, Department of Chemical Engineering, Materials and Industrial Production (DICMaPI), University of Naples Federico II, Piazzale Tecchio 80, 80125 Naples, ItalyDepartment of Agricultural Sciences, University of Naples Federico II, Via Università 100, Portici, 80055 Naples, ItalyA significant bottleneck for the industrial application of lipases stems from their poor stability in the presence of commercial triglycerides. This is mainly due to the inactivating effect of the products of triglyceride oxidation (PTO), which are usually produced when oils and fats, being imported from far countries, are stored for long periods. In this study, the immobilization of a lipase from <i>Candida rugosa</i> on chitosan hydrogels has been carried out following two alternative approaches based on the enzyme adsorption and entrapment to increase the lipase stability under the operating conditions that are typical of oleochemical transformations. The effect of model compounds representing different classes of PTO on a lipase has been studied to optimize the enzyme immobilization method. Particular attention has been devoted to the characterization of the inactivating effect of PTO in nonaqueous media, which are adopted for most industrial applications of lipases.https://www.mdpi.com/2310-2861/9/10/776biopolymercross-linkingchitosan hydrogeladsorptionentrapmentlipase |
spellingShingle | Domenico Pirozzi Alessandro Latte Filomena Sannino Immobilization of Lipases on Chitosan Hydrogels Improves Their Stability in the Presence of the Products of Triglyceride Oxidation Gels biopolymer cross-linking chitosan hydrogel adsorption entrapment lipase |
title | Immobilization of Lipases on Chitosan Hydrogels Improves Their Stability in the Presence of the Products of Triglyceride Oxidation |
title_full | Immobilization of Lipases on Chitosan Hydrogels Improves Their Stability in the Presence of the Products of Triglyceride Oxidation |
title_fullStr | Immobilization of Lipases on Chitosan Hydrogels Improves Their Stability in the Presence of the Products of Triglyceride Oxidation |
title_full_unstemmed | Immobilization of Lipases on Chitosan Hydrogels Improves Their Stability in the Presence of the Products of Triglyceride Oxidation |
title_short | Immobilization of Lipases on Chitosan Hydrogels Improves Their Stability in the Presence of the Products of Triglyceride Oxidation |
title_sort | immobilization of lipases on chitosan hydrogels improves their stability in the presence of the products of triglyceride oxidation |
topic | biopolymer cross-linking chitosan hydrogel adsorption entrapment lipase |
url | https://www.mdpi.com/2310-2861/9/10/776 |
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