Opportunities and Challenges in Doubled Haploids and Haploid Inducer-Mediated Genome-Editing Systems in Cucurbits
Doubled haploids have played a major role in cucurbit breeding for the past four decades. In situ parthenogenesis via irradiated pollen is the preferred technique to obtain haploid plantlets whose chromosomes are then doubled in Cucurbitaceae, such as melon, cucumber, pumpkin, squash and winter squa...
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| Format: | Article |
| Language: | English |
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MDPI AG
2020-09-01
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| Series: | Agronomy |
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| Online Access: | https://www.mdpi.com/2073-4395/10/9/1441 |
| _version_ | 1827705734615269376 |
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| author | Isidre Hooghvorst Salvador Nogués |
| author_facet | Isidre Hooghvorst Salvador Nogués |
| author_sort | Isidre Hooghvorst |
| collection | DOAJ |
| description | Doubled haploids have played a major role in cucurbit breeding for the past four decades. In situ parthenogenesis via irradiated pollen is the preferred technique to obtain haploid plantlets whose chromosomes are then doubled in Cucurbitaceae, such as melon, cucumber, pumpkin, squash and winter squash. In contrast to doubled haploid procedures in other species, in situ parthenogenesis in cucurbits presents many limiting factors which impede efficient production of haploids. In addition, it is very time-consuming and labor-intensive. However, the haploid inducer-mediated genome-editing system is a breakthrough technology for producing doubled haploids. Several reports have described using the CRISPR/Cas9 system in cucurbit species, and although its application has many bottlenecks, the targeted knock-out of the CENH3 gene will allow breeders to obtain haploid inducer lines that can be used to obtain parthenogenetic embryos. In this review, we discuss the progress made towards the development of doubled haploids and haploid inducer genotypes using CRISPR/Cas9 technologies in cucurbit species. The present review provides insights for the application of haploid inducer-mediated genome-editing system in cucurbit species |
| first_indexed | 2024-03-10T16:09:58Z |
| format | Article |
| id | doaj.art-bb2795eaf81e4d968340d9053a936f48 |
| institution | Directory Open Access Journal |
| issn | 2073-4395 |
| language | English |
| last_indexed | 2024-03-10T16:09:58Z |
| publishDate | 2020-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Agronomy |
| spelling | doaj.art-bb2795eaf81e4d968340d9053a936f482023-11-20T14:36:39ZengMDPI AGAgronomy2073-43952020-09-01109144110.3390/agronomy10091441Opportunities and Challenges in Doubled Haploids and Haploid Inducer-Mediated Genome-Editing Systems in CucurbitsIsidre Hooghvorst0Salvador Nogués1Departament de Biologia Evolutiva, Ecologia i Ciencies Ambientals, Secció de Fisiologia Vegetal, Universitat de Barcelona, 08028 Barcelona, SpainDepartament de Biologia Evolutiva, Ecologia i Ciencies Ambientals, Secció de Fisiologia Vegetal, Universitat de Barcelona, 08028 Barcelona, SpainDoubled haploids have played a major role in cucurbit breeding for the past four decades. In situ parthenogenesis via irradiated pollen is the preferred technique to obtain haploid plantlets whose chromosomes are then doubled in Cucurbitaceae, such as melon, cucumber, pumpkin, squash and winter squash. In contrast to doubled haploid procedures in other species, in situ parthenogenesis in cucurbits presents many limiting factors which impede efficient production of haploids. In addition, it is very time-consuming and labor-intensive. However, the haploid inducer-mediated genome-editing system is a breakthrough technology for producing doubled haploids. Several reports have described using the CRISPR/Cas9 system in cucurbit species, and although its application has many bottlenecks, the targeted knock-out of the CENH3 gene will allow breeders to obtain haploid inducer lines that can be used to obtain parthenogenetic embryos. In this review, we discuss the progress made towards the development of doubled haploids and haploid inducer genotypes using CRISPR/Cas9 technologies in cucurbit species. The present review provides insights for the application of haploid inducer-mediated genome-editing system in cucurbit specieshttps://www.mdpi.com/2073-4395/10/9/1441cucurbitsdoubled haploidsparthenogenesisgenome editingCRISPR/Cas9haploid-inducer |
| spellingShingle | Isidre Hooghvorst Salvador Nogués Opportunities and Challenges in Doubled Haploids and Haploid Inducer-Mediated Genome-Editing Systems in Cucurbits Agronomy cucurbits doubled haploids parthenogenesis genome editing CRISPR/Cas9 haploid-inducer |
| title | Opportunities and Challenges in Doubled Haploids and Haploid Inducer-Mediated Genome-Editing Systems in Cucurbits |
| title_full | Opportunities and Challenges in Doubled Haploids and Haploid Inducer-Mediated Genome-Editing Systems in Cucurbits |
| title_fullStr | Opportunities and Challenges in Doubled Haploids and Haploid Inducer-Mediated Genome-Editing Systems in Cucurbits |
| title_full_unstemmed | Opportunities and Challenges in Doubled Haploids and Haploid Inducer-Mediated Genome-Editing Systems in Cucurbits |
| title_short | Opportunities and Challenges in Doubled Haploids and Haploid Inducer-Mediated Genome-Editing Systems in Cucurbits |
| title_sort | opportunities and challenges in doubled haploids and haploid inducer mediated genome editing systems in cucurbits |
| topic | cucurbits doubled haploids parthenogenesis genome editing CRISPR/Cas9 haploid-inducer |
| url | https://www.mdpi.com/2073-4395/10/9/1441 |
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