Eu-doped carbon quantum dot as a selective probe for visualizing and monitoring sulfite in biological systems
The detection of SO32− in complex environments and its visualization at the cellular level are critical for understanding its role in biological processes. In this study, we developed an Eu-doped long-wavelength fluorescent carbon quantum dot (CD2) and investigated the detection mechanism, interfere...
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Frontiers Media S.A.
2023-12-01
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| Series: | Frontiers in Bioengineering and Biotechnology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fbioe.2023.1292136/full |
| _version_ | 1797400680406712320 |
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| author | Bo Kan Li Li Jiaoyu Hou Shuyan Liu Zhenwei Tian Qianchuang Sun |
| author_facet | Bo Kan Li Li Jiaoyu Hou Shuyan Liu Zhenwei Tian Qianchuang Sun |
| author_sort | Bo Kan |
| collection | DOAJ |
| description | The detection of SO32− in complex environments and its visualization at the cellular level are critical for understanding its role in biological processes. In this study, we developed an Eu-doped long-wavelength fluorescent carbon quantum dot (CD2) and investigated the detection mechanism, interference effects and cellular imaging applications of the fluorescent probe CD2. The results show that the addition of SO32− induces an electronic rearrangement that restores CD2 to its original structure, leading to a rapid increase in fluorescence intensity. Selectivity experiments showed that CD2 has excellent selectivity to SO32−, with minimal interference from common anions. In addition, CD2 shows good biocompatibility for cellular imaging applications, as evidenced by the high cell viability observed in HeLa cells. Using confocal microscopy, we detected a significant enhancement of red fluorescence in HeLa cells after addition of exogenous SO32−, demonstrating the potential of CD2 as a probe for monitoring cellular SO32− levels. These findings highlight the promise of CD2 as a selective SO32− detection probe in complex environments and its utility in cellular imaging studies. Further studies are necessary to fully exploit the potential of CD2 in various biological and biomedical applications. |
| first_indexed | 2024-03-09T01:58:58Z |
| format | Article |
| id | doaj.art-bb2d5ea81c21473c804166c0070aa19b |
| institution | Directory Open Access Journal |
| issn | 2296-4185 |
| language | English |
| last_indexed | 2024-03-09T01:58:58Z |
| publishDate | 2023-12-01 |
| publisher | Frontiers Media S.A. |
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| series | Frontiers in Bioengineering and Biotechnology |
| spelling | doaj.art-bb2d5ea81c21473c804166c0070aa19b2023-12-08T10:11:51ZengFrontiers Media S.A.Frontiers in Bioengineering and Biotechnology2296-41852023-12-011110.3389/fbioe.2023.12921361292136Eu-doped carbon quantum dot as a selective probe for visualizing and monitoring sulfite in biological systemsBo Kan0Li Li1Jiaoyu Hou2Shuyan Liu3Zhenwei Tian4Qianchuang Sun5Department of Clinical Laboratory, The Second Hospital of Jilin University, Changchun, Jilin, ChinaCollege of Chemistry, Jilin University, Changchun, Jilin, ChinaDepartment of Geriatrics, The First Hospital of Jilin University, Changchun, Jilin, ChinaDepartment of Ophthalmology, The Second Hospital of Jilin University, Changchun, Jilin, ChinaDepartment of Emergency and Critical Care, The Second Hospital of Jilin University, Changchun, ChinaDepartment of Anesthesiology, The Second Hospital of Jilin University, Changchun, Jilin, ChinaThe detection of SO32− in complex environments and its visualization at the cellular level are critical for understanding its role in biological processes. In this study, we developed an Eu-doped long-wavelength fluorescent carbon quantum dot (CD2) and investigated the detection mechanism, interference effects and cellular imaging applications of the fluorescent probe CD2. The results show that the addition of SO32− induces an electronic rearrangement that restores CD2 to its original structure, leading to a rapid increase in fluorescence intensity. Selectivity experiments showed that CD2 has excellent selectivity to SO32−, with minimal interference from common anions. In addition, CD2 shows good biocompatibility for cellular imaging applications, as evidenced by the high cell viability observed in HeLa cells. Using confocal microscopy, we detected a significant enhancement of red fluorescence in HeLa cells after addition of exogenous SO32−, demonstrating the potential of CD2 as a probe for monitoring cellular SO32− levels. These findings highlight the promise of CD2 as a selective SO32− detection probe in complex environments and its utility in cellular imaging studies. Further studies are necessary to fully exploit the potential of CD2 in various biological and biomedical applications.https://www.frontiersin.org/articles/10.3389/fbioe.2023.1292136/fullfluorescent probeselectivitycellular imagingsulfite detectionbiocompatibility |
| spellingShingle | Bo Kan Li Li Jiaoyu Hou Shuyan Liu Zhenwei Tian Qianchuang Sun Eu-doped carbon quantum dot as a selective probe for visualizing and monitoring sulfite in biological systems Frontiers in Bioengineering and Biotechnology fluorescent probe selectivity cellular imaging sulfite detection biocompatibility |
| title | Eu-doped carbon quantum dot as a selective probe for visualizing and monitoring sulfite in biological systems |
| title_full | Eu-doped carbon quantum dot as a selective probe for visualizing and monitoring sulfite in biological systems |
| title_fullStr | Eu-doped carbon quantum dot as a selective probe for visualizing and monitoring sulfite in biological systems |
| title_full_unstemmed | Eu-doped carbon quantum dot as a selective probe for visualizing and monitoring sulfite in biological systems |
| title_short | Eu-doped carbon quantum dot as a selective probe for visualizing and monitoring sulfite in biological systems |
| title_sort | eu doped carbon quantum dot as a selective probe for visualizing and monitoring sulfite in biological systems |
| topic | fluorescent probe selectivity cellular imaging sulfite detection biocompatibility |
| url | https://www.frontiersin.org/articles/10.3389/fbioe.2023.1292136/full |
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