Biochemical characterization of digestive β-glucosidase from midgut of Leiptinotarsa decemlineata (Coleoptera: Chrysomelidae)
The Colorado potato beetle, Leptinotarsa decemlineata Say (Col.: Chrysomelidae) is an important pest of potato worldwide and study of its glucosidases is an important step to develop appropriate pest control strategies. In this study, some biochemical aspects of β-glucosidase in the Colorado potato...
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| Format: | Article |
| Language: | English |
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University of Tarbiat Modares
2014-06-01
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| Series: | Journal of Crop Protection |
| Subjects: | |
| Online Access: | http://jcp.modares.ac.ir/article-3-198-en.html |
| _version_ | 1818474599911784448 |
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| author | Fahimeh Dehghanikhah Majid Kazzazi Hossein Madadi Vahid Hossein naveh |
| author_facet | Fahimeh Dehghanikhah Majid Kazzazi Hossein Madadi Vahid Hossein naveh |
| author_sort | Fahimeh Dehghanikhah |
| collection | DOAJ |
| description | The Colorado potato beetle, Leptinotarsa decemlineata Say (Col.: Chrysomelidae) is an important pest of potato worldwide and study of its glucosidases is an important step to develop appropriate pest control strategies. In this study, some biochemical aspects of β-glucosidase in the Colorado potato beetle were determined. The results showed that β-glucosidase activity in the midgut of adults was 6.68 Umg-1. Maximum activity of midgut β-glucosidase occurred at pH 4 to pH 5.5; however, the enzyme is active at pH 3 to pH 7 more than 50% of its relative activity. The enzyme was stable at pH 3 to pH 8 for 2 and 8 hours incubation time. According to the results, optimal temperature for the enzyme activity was 50 °Cand its stability significantly was reduced at 50 °C during 1 to 8 days incubation time. The enzyme activity decreased with the addition of different concentrations of MgCl2, urea, Tris and CaCl2. Enzyme activity was highly decreased at low concentrations of SDS (1 mM). |
| first_indexed | 2024-04-14T04:39:19Z |
| format | Article |
| id | doaj.art-bb2ed56be8104d0bad5ad9cd51efbf2d |
| institution | Directory Open Access Journal |
| issn | 2251-9041 2251-905X |
| language | English |
| last_indexed | 2024-04-14T04:39:19Z |
| publishDate | 2014-06-01 |
| publisher | University of Tarbiat Modares |
| record_format | Article |
| series | Journal of Crop Protection |
| spelling | doaj.art-bb2ed56be8104d0bad5ad9cd51efbf2d2022-12-22T02:11:45ZengUniversity of Tarbiat ModaresJournal of Crop Protection2251-90412251-905X2014-06-0132181189Biochemical characterization of digestive β-glucosidase from midgut of Leiptinotarsa decemlineata (Coleoptera: Chrysomelidae)Fahimeh Dehghanikhah0Majid Kazzazi1Hossein Madadi2Vahid Hossein naveh3 Department of Plant Protection, Faculty of Agriculture, Bu-Ali Sina University, Hamedan, Iran. Department of Plant Protection, Faculty of Agriculture, Bu-Ali Sina University, Hamedan, Iran. Department of Plant Protection, Faculty of Agriculture, Bu-Ali Sina University, Hamedan, Iran. Department of Plant Protection, College of Agriculture, University of Tehran; Karaj, Iran. The Colorado potato beetle, Leptinotarsa decemlineata Say (Col.: Chrysomelidae) is an important pest of potato worldwide and study of its glucosidases is an important step to develop appropriate pest control strategies. In this study, some biochemical aspects of β-glucosidase in the Colorado potato beetle were determined. The results showed that β-glucosidase activity in the midgut of adults was 6.68 Umg-1. Maximum activity of midgut β-glucosidase occurred at pH 4 to pH 5.5; however, the enzyme is active at pH 3 to pH 7 more than 50% of its relative activity. The enzyme was stable at pH 3 to pH 8 for 2 and 8 hours incubation time. According to the results, optimal temperature for the enzyme activity was 50 °Cand its stability significantly was reduced at 50 °C during 1 to 8 days incubation time. The enzyme activity decreased with the addition of different concentrations of MgCl2, urea, Tris and CaCl2. Enzyme activity was highly decreased at low concentrations of SDS (1 mM).http://jcp.modares.ac.ir/article-3-198-en.htmlbiochemistrymidgutβ-glucosidaseleptinotarsa decemlineata |
| spellingShingle | Fahimeh Dehghanikhah Majid Kazzazi Hossein Madadi Vahid Hossein naveh Biochemical characterization of digestive β-glucosidase from midgut of Leiptinotarsa decemlineata (Coleoptera: Chrysomelidae) Journal of Crop Protection biochemistry midgut β-glucosidase leptinotarsa decemlineata |
| title | Biochemical characterization of digestive β-glucosidase from midgut of Leiptinotarsa decemlineata (Coleoptera: Chrysomelidae) |
| title_full | Biochemical characterization of digestive β-glucosidase from midgut of Leiptinotarsa decemlineata (Coleoptera: Chrysomelidae) |
| title_fullStr | Biochemical characterization of digestive β-glucosidase from midgut of Leiptinotarsa decemlineata (Coleoptera: Chrysomelidae) |
| title_full_unstemmed | Biochemical characterization of digestive β-glucosidase from midgut of Leiptinotarsa decemlineata (Coleoptera: Chrysomelidae) |
| title_short | Biochemical characterization of digestive β-glucosidase from midgut of Leiptinotarsa decemlineata (Coleoptera: Chrysomelidae) |
| title_sort | biochemical characterization of digestive β glucosidase from midgut of leiptinotarsa decemlineata coleoptera chrysomelidae |
| topic | biochemistry midgut β-glucosidase leptinotarsa decemlineata |
| url | http://jcp.modares.ac.ir/article-3-198-en.html |
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