Modeling the Architecture of Depolymerase-Containing Receptor Binding Proteins in Klebsiella Phages
Klebsiella pneumoniae carries a thick polysaccharide capsule. This highly variable chemical structure plays an important role in its virulence. Many Klebsiella bacteriophages recognize this capsule with a receptor binding protein (RBP) that contains a depolymerase domain. This domain degrades the ca...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2019-11-01
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| Series: | Frontiers in Microbiology |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/article/10.3389/fmicb.2019.02649/full |
| _version_ | 1818841141223620608 |
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| author | Agnieszka Latka Agnieszka Latka Petr G. Leiman Zuzanna Drulis-Kawa Yves Briers |
| author_facet | Agnieszka Latka Agnieszka Latka Petr G. Leiman Zuzanna Drulis-Kawa Yves Briers |
| author_sort | Agnieszka Latka |
| collection | DOAJ |
| description | Klebsiella pneumoniae carries a thick polysaccharide capsule. This highly variable chemical structure plays an important role in its virulence. Many Klebsiella bacteriophages recognize this capsule with a receptor binding protein (RBP) that contains a depolymerase domain. This domain degrades the capsule to initiate phage infection. RBPs are highly specific and thus largely determine the host spectrum of the phage. A majority of known Klebsiella phages have only one or two RBPs, but phages with up to 11 RBPs with depolymerase activity and a broad host spectrum have been identified. A detailed bioinformatic analysis shows that similar RBP domains repeatedly occur in K. pneumoniae phages with structural RBP domains for attachment of an RBP to the phage tail (anchor domain) or for branching of RBPs (T4gp10-like domain). Structural domains determining the RBP architecture are located at the N-terminus, while the depolymerase is located in the center of protein. Occasionally, the RBP is complemented with an autocleavable chaperone domain at the distal end serving for folding and multimerization. The enzymatic domain is subjected to an intense horizontal transfer to rapidly shift the phage host spectrum without affecting the RBP architecture. These analyses allowed to model a set of conserved RBP architectures, indicating evolutionary linkages. |
| first_indexed | 2024-12-19T04:21:21Z |
| format | Article |
| id | doaj.art-bb2ffbeb6b62480f8469206af784e14d |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-12-19T04:21:21Z |
| publishDate | 2019-11-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-bb2ffbeb6b62480f8469206af784e14d2022-12-21T20:36:09ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2019-11-011010.3389/fmicb.2019.02649484384Modeling the Architecture of Depolymerase-Containing Receptor Binding Proteins in Klebsiella PhagesAgnieszka Latka0Agnieszka Latka1Petr G. Leiman2Zuzanna Drulis-Kawa3Yves Briers4Laboratory of Applied Biotechnology, Department of Biotechnology, Ghent University, Ghent, BelgiumDepartment of Pathogen Biology and Immunology, Institute of Genetics and Microbiology, University of Wrocław, Wrocław, PolandSealy Center for Structural Biology and Molecular Biophysics, Department of Biochemistry and Molecular Biology, The University of Texas Medical Branch, Galveston, TX, United StatesDepartment of Pathogen Biology and Immunology, Institute of Genetics and Microbiology, University of Wrocław, Wrocław, PolandLaboratory of Applied Biotechnology, Department of Biotechnology, Ghent University, Ghent, BelgiumKlebsiella pneumoniae carries a thick polysaccharide capsule. This highly variable chemical structure plays an important role in its virulence. Many Klebsiella bacteriophages recognize this capsule with a receptor binding protein (RBP) that contains a depolymerase domain. This domain degrades the capsule to initiate phage infection. RBPs are highly specific and thus largely determine the host spectrum of the phage. A majority of known Klebsiella phages have only one or two RBPs, but phages with up to 11 RBPs with depolymerase activity and a broad host spectrum have been identified. A detailed bioinformatic analysis shows that similar RBP domains repeatedly occur in K. pneumoniae phages with structural RBP domains for attachment of an RBP to the phage tail (anchor domain) or for branching of RBPs (T4gp10-like domain). Structural domains determining the RBP architecture are located at the N-terminus, while the depolymerase is located in the center of protein. Occasionally, the RBP is complemented with an autocleavable chaperone domain at the distal end serving for folding and multimerization. The enzymatic domain is subjected to an intense horizontal transfer to rapidly shift the phage host spectrum without affecting the RBP architecture. These analyses allowed to model a set of conserved RBP architectures, indicating evolutionary linkages.https://www.frontiersin.org/article/10.3389/fmicb.2019.02649/fullhorizontal transfertail fiber genesreceptor binding proteinphage evolutiondepolymerase |
| spellingShingle | Agnieszka Latka Agnieszka Latka Petr G. Leiman Zuzanna Drulis-Kawa Yves Briers Modeling the Architecture of Depolymerase-Containing Receptor Binding Proteins in Klebsiella Phages Frontiers in Microbiology horizontal transfer tail fiber genes receptor binding protein phage evolution depolymerase |
| title | Modeling the Architecture of Depolymerase-Containing Receptor Binding Proteins in Klebsiella Phages |
| title_full | Modeling the Architecture of Depolymerase-Containing Receptor Binding Proteins in Klebsiella Phages |
| title_fullStr | Modeling the Architecture of Depolymerase-Containing Receptor Binding Proteins in Klebsiella Phages |
| title_full_unstemmed | Modeling the Architecture of Depolymerase-Containing Receptor Binding Proteins in Klebsiella Phages |
| title_short | Modeling the Architecture of Depolymerase-Containing Receptor Binding Proteins in Klebsiella Phages |
| title_sort | modeling the architecture of depolymerase containing receptor binding proteins in klebsiella phages |
| topic | horizontal transfer tail fiber genes receptor binding protein phage evolution depolymerase |
| url | https://www.frontiersin.org/article/10.3389/fmicb.2019.02649/full |
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