What is your count? An observational study of lymph node counting in 2,028 colorectal cancer resections.
<h4>Background</h4>Lymph node status and lymph node count (LNC) are predictors of colorectal cancer outcome. Under-sampling of lymph nodes may lead to clinically relevant stage migration.<h4>Methods</h4>Colorectal cancer (CRC) cases with a synoptic report, accessioned 2012-20...
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2024-01-01
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| Series: | PLoS ONE |
| Online Access: | https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0295209&type=printable |
| _version_ | 1797292843910299648 |
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| author | Shivali Srivastava Ipshita Kak Pierre Major Michael Bonert |
| author_facet | Shivali Srivastava Ipshita Kak Pierre Major Michael Bonert |
| author_sort | Shivali Srivastava |
| collection | DOAJ |
| description | <h4>Background</h4>Lymph node status and lymph node count (LNC) are predictors of colorectal cancer outcome. Under-sampling of lymph nodes may lead to clinically relevant stage migration.<h4>Methods</h4>Colorectal cancer (CRC) cases with a synoptic report, accessioned 2012-2020 at a regional laboratory, were extracted and retrospectively studied. LNC, positive lymph node count (PLNC), tumour deposits present (TDpos), and 'y' (staging) prefix (YS) were retrieved and tabulated by pathologist using custom software. Statistical analyses were done with R.<h4>Data and results</h4>The cohort had 2,543 CRC resections. Seventeen pathologists interpreted >50 cases (range: 56-356) each and collectively saw 2,074. After cases with unavailable data were purged, 2,028 cases remained with 43,996 lymph nodes, of which 2,637/43,996 were positive. 368 cases had a 'y' prefix, and 379 had TDpos. The 17 pathologists' median LNC/case was 19.0 (range: 14.0-24.0), and the mean PLNC per case was 1.4 (range: 1.0-2.0). Kruskal-Wallis rank sum tests showed there were differences in LNC (p<0.001) among pathologists; however, PLNC did not show this association (p = 0.2917). T-tests showed that mean LNC (p<0.001) and PLNC (p<0.035) differed between YS. 138 of 2,028 cases had less than the 12 LNC target. Logistic regression revealed a strong association between meeting the LNC target and pathologist (p<0.001) but TDpos was non-predictive (p = 0.4736).<h4>Conclusions</h4>Positive lymph node call rate has a good consistency in the laboratory; however, lymph node count varies significantly between pathologists. Standardized counting criteria are needed to improve uniformity and could be aided by synoptic reporting data. |
| first_indexed | 2024-03-07T20:03:24Z |
| format | Article |
| id | doaj.art-bb30d833d1d34b998c54da14074b982b |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-03-07T20:03:24Z |
| publishDate | 2024-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-bb30d833d1d34b998c54da14074b982b2024-02-28T05:31:46ZengPublic Library of Science (PLoS)PLoS ONE1932-62032024-01-01192e029520910.1371/journal.pone.0295209What is your count? An observational study of lymph node counting in 2,028 colorectal cancer resections.Shivali SrivastavaIpshita KakPierre MajorMichael Bonert<h4>Background</h4>Lymph node status and lymph node count (LNC) are predictors of colorectal cancer outcome. Under-sampling of lymph nodes may lead to clinically relevant stage migration.<h4>Methods</h4>Colorectal cancer (CRC) cases with a synoptic report, accessioned 2012-2020 at a regional laboratory, were extracted and retrospectively studied. LNC, positive lymph node count (PLNC), tumour deposits present (TDpos), and 'y' (staging) prefix (YS) were retrieved and tabulated by pathologist using custom software. Statistical analyses were done with R.<h4>Data and results</h4>The cohort had 2,543 CRC resections. Seventeen pathologists interpreted >50 cases (range: 56-356) each and collectively saw 2,074. After cases with unavailable data were purged, 2,028 cases remained with 43,996 lymph nodes, of which 2,637/43,996 were positive. 368 cases had a 'y' prefix, and 379 had TDpos. The 17 pathologists' median LNC/case was 19.0 (range: 14.0-24.0), and the mean PLNC per case was 1.4 (range: 1.0-2.0). Kruskal-Wallis rank sum tests showed there were differences in LNC (p<0.001) among pathologists; however, PLNC did not show this association (p = 0.2917). T-tests showed that mean LNC (p<0.001) and PLNC (p<0.035) differed between YS. 138 of 2,028 cases had less than the 12 LNC target. Logistic regression revealed a strong association between meeting the LNC target and pathologist (p<0.001) but TDpos was non-predictive (p = 0.4736).<h4>Conclusions</h4>Positive lymph node call rate has a good consistency in the laboratory; however, lymph node count varies significantly between pathologists. Standardized counting criteria are needed to improve uniformity and could be aided by synoptic reporting data.https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0295209&type=printable |
| spellingShingle | Shivali Srivastava Ipshita Kak Pierre Major Michael Bonert What is your count? An observational study of lymph node counting in 2,028 colorectal cancer resections. PLoS ONE |
| title | What is your count? An observational study of lymph node counting in 2,028 colorectal cancer resections. |
| title_full | What is your count? An observational study of lymph node counting in 2,028 colorectal cancer resections. |
| title_fullStr | What is your count? An observational study of lymph node counting in 2,028 colorectal cancer resections. |
| title_full_unstemmed | What is your count? An observational study of lymph node counting in 2,028 colorectal cancer resections. |
| title_short | What is your count? An observational study of lymph node counting in 2,028 colorectal cancer resections. |
| title_sort | what is your count an observational study of lymph node counting in 2 028 colorectal cancer resections |
| url | https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0295209&type=printable |
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