<it>Bacillus coagulans</it> GBI-30, 6086 limits the recurrence of <it>Clostridium difficile</it>-Induced colitis following vancomycin withdrawal in mice
<p>Abstract</p> <p>Background</p> <p>Recently, we found that the probiotic strain <it>Bacillus coagulans</it> GBI-30, 6086 (GanedenBC<sup>30</sup>) improved indices of <it>Clostridium difficile (C. difficile)</it>-induced colitis in m...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2012-10-01
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| Series: | Gut Pathogens |
| Subjects: |
| _version_ | 1811284322713337856 |
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| author | Fitzpatrick Leo R Small Jeffrey S Greene Wallace H Karpa Kelly D Farmer Sean Keller David |
| author_facet | Fitzpatrick Leo R Small Jeffrey S Greene Wallace H Karpa Kelly D Farmer Sean Keller David |
| author_sort | Fitzpatrick Leo R |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p>Recently, we found that the probiotic strain <it>Bacillus coagulans</it> GBI-30, 6086 (GanedenBC<sup>30</sup>) improved indices of <it>Clostridium difficile (C. difficile)</it>-induced colitis in mice (Fitzpatrick et al., <it>Gut Pathogens</it>, 2011). Our goal was to determine if BC30 could also prevent the recurrence of <it>C. difficile</it>-induced colitis in mice, following initial treatment with vancomycin. During study days 0 through 5, mice were treated with antibiotics. On day 6, the <it>C. difficile</it> strain VPI 10463 was given by oro-gastric gavage at ≈ 5x10<sup>4</sup> CFU to induce colitis. Mice were treated on study days 6 to 10 with vancomycin (50 mg/kg) (vanco) or vehicle (saline) by gavage. On days 10 to16, mice were dosed by gavage with saline vehicle or BC30 (2 x 10<sup>9</sup> CFU per day). Mice were monitored for mortality, weight loss and diarrhea. On study days 14, 16 and 17, stools and colons were collected for analyzing other parameters of colitis.</p> <p>Results</p> <p>The mean stool consistency score in Vehicle/C.difficile/Vanco mice increased from 0.4 (day 10) to a range of 1.1 to 1.4 (days 14 to 17), indicating the recurrence of colitis. On days 13 through 17, the stool consistency scores for the vancomycin/BC30 mice were significantly lower (p< 0.05) than for the vancomycin/vehicle cohort of animals. On day 17, 88.9% of mice treated with BC30 had normal stools, while this value was 0% with vehicle treatment (p value = 0.0004). Colonic myeloperoxidase (Units/2 cm colon) was significantly (p < 0.05) reduced from 4.3 ± 0.7 (Vehicle/C.difficile/Vanco) to 2.6 ± 0.2 (BC30/C. Difficle/Vanco). The colonic histology score and Keratinocyte derived-chemokine level in the colon were also lower in BC30 treated mice.</p> <p>Summary</p> <p>In BC30-treated mice, there was evidence of better stool consistency, as well as improved biochemical and histological indices of colitis, following initial treatment of animals with vancomycin.</p> <p>Conclusion</p> <p>BC30 limited the recurrence of <it>CD</it>-induced colitis following vancomycin withdrawal in mice.</p> |
| first_indexed | 2024-04-13T02:26:57Z |
| format | Article |
| id | doaj.art-bb3210e418c244df8e34a549785b53b2 |
| institution | Directory Open Access Journal |
| issn | 1757-4749 |
| language | English |
| last_indexed | 2024-04-13T02:26:57Z |
| publishDate | 2012-10-01 |
| publisher | BMC |
| record_format | Article |
| series | Gut Pathogens |
| spelling | doaj.art-bb3210e418c244df8e34a549785b53b22022-12-22T03:06:44ZengBMCGut Pathogens1757-47492012-10-01411310.1186/1757-4749-4-13<it>Bacillus coagulans</it> GBI-30, 6086 limits the recurrence of <it>Clostridium difficile</it>-Induced colitis following vancomycin withdrawal in miceFitzpatrick Leo RSmall Jeffrey SGreene Wallace HKarpa Kelly DFarmer SeanKeller David<p>Abstract</p> <p>Background</p> <p>Recently, we found that the probiotic strain <it>Bacillus coagulans</it> GBI-30, 6086 (GanedenBC<sup>30</sup>) improved indices of <it>Clostridium difficile (C. difficile)</it>-induced colitis in mice (Fitzpatrick et al., <it>Gut Pathogens</it>, 2011). Our goal was to determine if BC30 could also prevent the recurrence of <it>C. difficile</it>-induced colitis in mice, following initial treatment with vancomycin. During study days 0 through 5, mice were treated with antibiotics. On day 6, the <it>C. difficile</it> strain VPI 10463 was given by oro-gastric gavage at ≈ 5x10<sup>4</sup> CFU to induce colitis. Mice were treated on study days 6 to 10 with vancomycin (50 mg/kg) (vanco) or vehicle (saline) by gavage. On days 10 to16, mice were dosed by gavage with saline vehicle or BC30 (2 x 10<sup>9</sup> CFU per day). Mice were monitored for mortality, weight loss and diarrhea. On study days 14, 16 and 17, stools and colons were collected for analyzing other parameters of colitis.</p> <p>Results</p> <p>The mean stool consistency score in Vehicle/C.difficile/Vanco mice increased from 0.4 (day 10) to a range of 1.1 to 1.4 (days 14 to 17), indicating the recurrence of colitis. On days 13 through 17, the stool consistency scores for the vancomycin/BC30 mice were significantly lower (p< 0.05) than for the vancomycin/vehicle cohort of animals. On day 17, 88.9% of mice treated with BC30 had normal stools, while this value was 0% with vehicle treatment (p value = 0.0004). Colonic myeloperoxidase (Units/2 cm colon) was significantly (p < 0.05) reduced from 4.3 ± 0.7 (Vehicle/C.difficile/Vanco) to 2.6 ± 0.2 (BC30/C. Difficle/Vanco). The colonic histology score and Keratinocyte derived-chemokine level in the colon were also lower in BC30 treated mice.</p> <p>Summary</p> <p>In BC30-treated mice, there was evidence of better stool consistency, as well as improved biochemical and histological indices of colitis, following initial treatment of animals with vancomycin.</p> <p>Conclusion</p> <p>BC30 limited the recurrence of <it>CD</it>-induced colitis following vancomycin withdrawal in mice.</p><it>Clostridium difficile</it>GanedenBC30ProbioticsColitisMice |
| spellingShingle | Fitzpatrick Leo R Small Jeffrey S Greene Wallace H Karpa Kelly D Farmer Sean Keller David <it>Bacillus coagulans</it> GBI-30, 6086 limits the recurrence of <it>Clostridium difficile</it>-Induced colitis following vancomycin withdrawal in mice Gut Pathogens <it>Clostridium difficile</it> GanedenBC30 Probiotics Colitis Mice |
| title | <it>Bacillus coagulans</it> GBI-30, 6086 limits the recurrence of <it>Clostridium difficile</it>-Induced colitis following vancomycin withdrawal in mice |
| title_full | <it>Bacillus coagulans</it> GBI-30, 6086 limits the recurrence of <it>Clostridium difficile</it>-Induced colitis following vancomycin withdrawal in mice |
| title_fullStr | <it>Bacillus coagulans</it> GBI-30, 6086 limits the recurrence of <it>Clostridium difficile</it>-Induced colitis following vancomycin withdrawal in mice |
| title_full_unstemmed | <it>Bacillus coagulans</it> GBI-30, 6086 limits the recurrence of <it>Clostridium difficile</it>-Induced colitis following vancomycin withdrawal in mice |
| title_short | <it>Bacillus coagulans</it> GBI-30, 6086 limits the recurrence of <it>Clostridium difficile</it>-Induced colitis following vancomycin withdrawal in mice |
| title_sort | it bacillus coagulans it gbi 30 6086 limits the recurrence of it clostridium difficile it induced colitis following vancomycin withdrawal in mice |
| topic | <it>Clostridium difficile</it> GanedenBC30 Probiotics Colitis Mice |
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