Variable Level of Dominance of Candidate Genes Controlling Drought Functional Traits in Maize Hybrids
Breeding maize for drought tolerance necessitates the knowledge on tolerant genotypes, molecular basis of drought tolerance mechanism, action, and expression pattern of genes. Studying the expression pattern and gene action of candidate genes during drought stress in the hybrids will help in choosin...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2017-06-01
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| Series: | Frontiers in Plant Science |
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| Online Access: | http://journal.frontiersin.org/article/10.3389/fpls.2017.00940/full |
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| author | Ha Van Gioi Ha Van Gioi Mallana Gowdra Mallikarjuna Mittal Shikha Banduni Pooja Shailendra K. Jha Prasanta K. Dash Arunkumar M. Basappa Raveendra N. Gadag Atmakuri Ramakrishna Rao Thirunavukkarasu Nepolean |
| author_facet | Ha Van Gioi Ha Van Gioi Mallana Gowdra Mallikarjuna Mittal Shikha Banduni Pooja Shailendra K. Jha Prasanta K. Dash Arunkumar M. Basappa Raveendra N. Gadag Atmakuri Ramakrishna Rao Thirunavukkarasu Nepolean |
| author_sort | Ha Van Gioi |
| collection | DOAJ |
| description | Breeding maize for drought tolerance necessitates the knowledge on tolerant genotypes, molecular basis of drought tolerance mechanism, action, and expression pattern of genes. Studying the expression pattern and gene action of candidate genes during drought stress in the hybrids will help in choosing target genes for drought tolerance breeding. In the present investigation, a set of five hybrids and their seven parents with a variable level of tolerance to drought stress was selected to study the magnitude and the direction of 52 drought-responsive candidate genes distributed across various biological functions, viz., stomatal regulation, root development, detoxification, hormone signaling, photosynthesis, and sugar metabolism. The tolerant parents, HKI1105 and CML425, and their hybrid, ADWLH2, were physiologically active under drought stress, since vital parameters viz., chlorophyll, root length and relative water content, were on par with the respective well-watered control. All the genes were up-regulated in ADWLH2, many were down-regulated in HM8 and HM9, and most were down-regulated in PMH1 and PMH3 in the shoots and roots. The nature of the gene action was controlled by the parental combination rather than the parent per se. The differentially expressed genes in all five hybrids explained a mostly non-additive gene action over additivity, which was skewed toward any of the parental lines. Tissue-specific gene action was also noticed in many of the genes. The non-additive gene action is driven by genetic diversity, allele polymorphism, events during gene regulation, and small RNAs under the stress condition. Differential regulation and cross-talk of genes controlling various biological functions explained the basis of drought tolerance in subtropical maize hybrids. The nature of the gene action and the direction of the expression play crucial roles in designing introgression and hybrid breeding programmes to breed drought tolerant maize hybrids. |
| first_indexed | 2024-12-20T09:47:54Z |
| format | Article |
| id | doaj.art-bb337b723bf04083ab0c145929ed84c0 |
| institution | Directory Open Access Journal |
| issn | 1664-462X |
| language | English |
| last_indexed | 2024-12-20T09:47:54Z |
| publishDate | 2017-06-01 |
| publisher | Frontiers Media S.A. |
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| series | Frontiers in Plant Science |
| spelling | doaj.art-bb337b723bf04083ab0c145929ed84c02022-12-21T19:44:41ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2017-06-01810.3389/fpls.2017.00940261590Variable Level of Dominance of Candidate Genes Controlling Drought Functional Traits in Maize HybridsHa Van Gioi0Ha Van Gioi1Mallana Gowdra Mallikarjuna2Mittal Shikha3Banduni Pooja4Shailendra K. Jha5Prasanta K. Dash6Arunkumar M. Basappa7Raveendra N. Gadag8Atmakuri Ramakrishna Rao9Thirunavukkarasu Nepolean10Division of Genetics, Indian Agricultural Research Institute (ICAR)New Delhi, IndiaForage Crops Department, Maize Research InstituteHa Noi, VietnamDivision of Genetics, Indian Agricultural Research Institute (ICAR)New Delhi, IndiaDivision of Genetics, Indian Agricultural Research Institute (ICAR)New Delhi, IndiaDivision of Genetics, Indian Agricultural Research Institute (ICAR)New Delhi, IndiaDivision of Genetics, Indian Agricultural Research Institute (ICAR)New Delhi, IndiaNational Research Centre on Plant Biotechnology (ICAR)New Delhi, IndiaDivision of Seed Science and Technology, Indian Agricultural Research Institute (ICAR)New Delhi, IndiaDivision of Genetics, Indian Agricultural Research Institute (ICAR)New Delhi, IndiaCentre for Agricultural Bioinformatics, Indian Agricultural Statistics Research Institute (ICAR)New Delhi, IndiaDivision of Genetics, Indian Agricultural Research Institute (ICAR)New Delhi, IndiaBreeding maize for drought tolerance necessitates the knowledge on tolerant genotypes, molecular basis of drought tolerance mechanism, action, and expression pattern of genes. Studying the expression pattern and gene action of candidate genes during drought stress in the hybrids will help in choosing target genes for drought tolerance breeding. In the present investigation, a set of five hybrids and their seven parents with a variable level of tolerance to drought stress was selected to study the magnitude and the direction of 52 drought-responsive candidate genes distributed across various biological functions, viz., stomatal regulation, root development, detoxification, hormone signaling, photosynthesis, and sugar metabolism. The tolerant parents, HKI1105 and CML425, and their hybrid, ADWLH2, were physiologically active under drought stress, since vital parameters viz., chlorophyll, root length and relative water content, were on par with the respective well-watered control. All the genes were up-regulated in ADWLH2, many were down-regulated in HM8 and HM9, and most were down-regulated in PMH1 and PMH3 in the shoots and roots. The nature of the gene action was controlled by the parental combination rather than the parent per se. The differentially expressed genes in all five hybrids explained a mostly non-additive gene action over additivity, which was skewed toward any of the parental lines. Tissue-specific gene action was also noticed in many of the genes. The non-additive gene action is driven by genetic diversity, allele polymorphism, events during gene regulation, and small RNAs under the stress condition. Differential regulation and cross-talk of genes controlling various biological functions explained the basis of drought tolerance in subtropical maize hybrids. The nature of the gene action and the direction of the expression play crucial roles in designing introgression and hybrid breeding programmes to breed drought tolerant maize hybrids.http://journal.frontiersin.org/article/10.3389/fpls.2017.00940/fulladditiveadaptive traitscandidate genesdominancedroughtfunctional traits |
| spellingShingle | Ha Van Gioi Ha Van Gioi Mallana Gowdra Mallikarjuna Mittal Shikha Banduni Pooja Shailendra K. Jha Prasanta K. Dash Arunkumar M. Basappa Raveendra N. Gadag Atmakuri Ramakrishna Rao Thirunavukkarasu Nepolean Variable Level of Dominance of Candidate Genes Controlling Drought Functional Traits in Maize Hybrids Frontiers in Plant Science additive adaptive traits candidate genes dominance drought functional traits |
| title | Variable Level of Dominance of Candidate Genes Controlling Drought Functional Traits in Maize Hybrids |
| title_full | Variable Level of Dominance of Candidate Genes Controlling Drought Functional Traits in Maize Hybrids |
| title_fullStr | Variable Level of Dominance of Candidate Genes Controlling Drought Functional Traits in Maize Hybrids |
| title_full_unstemmed | Variable Level of Dominance of Candidate Genes Controlling Drought Functional Traits in Maize Hybrids |
| title_short | Variable Level of Dominance of Candidate Genes Controlling Drought Functional Traits in Maize Hybrids |
| title_sort | variable level of dominance of candidate genes controlling drought functional traits in maize hybrids |
| topic | additive adaptive traits candidate genes dominance drought functional traits |
| url | http://journal.frontiersin.org/article/10.3389/fpls.2017.00940/full |
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