A GBS-Based GWAS Analysis of Leaf and Stripe Rust Resistance in Diverse Pre-Breeding Germplasm of Bread Wheat (<i>Triticum aestivum</i> L.)
Yellow (YR) and leaf (LR) rusts caused by <i>Puccinia striiformis</i> f. sp. <i>tritici</i> (Pst) and <i>Puccinia triticina</i>, respectively, are of utmost importance to wheat producers because of their qualitative and quantitative effect on yield. The search for...
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MDPI AG
2022-09-01
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| Series: | Plants |
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| Online Access: | https://www.mdpi.com/2223-7747/11/18/2363 |
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| author | Kamran Saleem Sajid Shokat Muhammad Qandeel Waheed Hafiz Muhammad Imran Arshad Mian Abdur Rehman Arif |
| author_facet | Kamran Saleem Sajid Shokat Muhammad Qandeel Waheed Hafiz Muhammad Imran Arshad Mian Abdur Rehman Arif |
| author_sort | Kamran Saleem |
| collection | DOAJ |
| description | Yellow (YR) and leaf (LR) rusts caused by <i>Puccinia striiformis</i> f. sp. <i>tritici</i> (Pst) and <i>Puccinia triticina</i>, respectively, are of utmost importance to wheat producers because of their qualitative and quantitative effect on yield. The search for new loci resistant to both rusts is an ongoing challenge faced by plant breeders and pathologists. Our investigation was conducted on a subset of 168 pre-breeding lines (PBLs) to identify the resistant germplasm against the prevalent local races of LR and YR under field conditions followed by its genetic mapping. Our analysis revealed a range of phenotypic responses towards both rusts. We identified 28 wheat lines with immune response and 85 resistant wheat genotypes against LR, whereas there were only eight immune and 52 resistant genotypes against YR. A GWAS (genome-wide association study) identified 190 marker-trait associations (MTAs), where 120 were specific to LR and 70 were specific to YR. These MTAs were confined to 86 quantitative trait loci (QTLs), where 50 QTLs carried MTAs associated with only LR, 29 QTLs carried MTAs associated with YR, and seven QTLs carried MTAs associated with both LR and YR. Possible candidate genes at the site of these QTLs are discussed. Overall, 70 PBLs carried all seven LR/YR QTLs. Furthermore, there were five PBLs with less than five scores for both LR and YR carrying positive alleles of all seven YR/LR QTLs, which are fit to be included in a breeding program for rust resistance induction. |
| first_indexed | 2024-03-09T22:47:42Z |
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| issn | 2223-7747 |
| language | English |
| last_indexed | 2024-03-09T22:47:42Z |
| publishDate | 2022-09-01 |
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| record_format | Article |
| series | Plants |
| spelling | doaj.art-bb353183bb83425b919e02b69579fc432023-11-23T18:26:35ZengMDPI AGPlants2223-77472022-09-011118236310.3390/plants11182363A GBS-Based GWAS Analysis of Leaf and Stripe Rust Resistance in Diverse Pre-Breeding Germplasm of Bread Wheat (<i>Triticum aestivum</i> L.)Kamran Saleem0Sajid Shokat1Muhammad Qandeel Waheed2Hafiz Muhammad Imran Arshad3Mian Abdur Rehman Arif4Molecular Phytopathology Group, Plant Protection Division, Nuclear Institute for Agriculture and Biology (NIAB), Faisalabad P.O. Box 128, PakistanWheat Breeding Group, Plant Breeding and Genetics Division, Nuclear Institute for Agriculture and Biology (NIAB), Faisalabad P.O. Box 128, PakistanWheat Breeding Group, Plant Breeding and Genetics Division, Nuclear Institute for Agriculture and Biology (NIAB), Faisalabad P.O. Box 128, PakistanMolecular Phytopathology Group, Plant Protection Division, Nuclear Institute for Agriculture and Biology (NIAB), Faisalabad P.O. Box 128, PakistanWheat Breeding Group, Plant Breeding and Genetics Division, Nuclear Institute for Agriculture and Biology (NIAB), Faisalabad P.O. Box 128, PakistanYellow (YR) and leaf (LR) rusts caused by <i>Puccinia striiformis</i> f. sp. <i>tritici</i> (Pst) and <i>Puccinia triticina</i>, respectively, are of utmost importance to wheat producers because of their qualitative and quantitative effect on yield. The search for new loci resistant to both rusts is an ongoing challenge faced by plant breeders and pathologists. Our investigation was conducted on a subset of 168 pre-breeding lines (PBLs) to identify the resistant germplasm against the prevalent local races of LR and YR under field conditions followed by its genetic mapping. Our analysis revealed a range of phenotypic responses towards both rusts. We identified 28 wheat lines with immune response and 85 resistant wheat genotypes against LR, whereas there were only eight immune and 52 resistant genotypes against YR. A GWAS (genome-wide association study) identified 190 marker-trait associations (MTAs), where 120 were specific to LR and 70 were specific to YR. These MTAs were confined to 86 quantitative trait loci (QTLs), where 50 QTLs carried MTAs associated with only LR, 29 QTLs carried MTAs associated with YR, and seven QTLs carried MTAs associated with both LR and YR. Possible candidate genes at the site of these QTLs are discussed. Overall, 70 PBLs carried all seven LR/YR QTLs. Furthermore, there were five PBLs with less than five scores for both LR and YR carrying positive alleles of all seven YR/LR QTLs, which are fit to be included in a breeding program for rust resistance induction.https://www.mdpi.com/2223-7747/11/18/2363leaf rustyellow rustwheatresistanceGWASbreeding |
| spellingShingle | Kamran Saleem Sajid Shokat Muhammad Qandeel Waheed Hafiz Muhammad Imran Arshad Mian Abdur Rehman Arif A GBS-Based GWAS Analysis of Leaf and Stripe Rust Resistance in Diverse Pre-Breeding Germplasm of Bread Wheat (<i>Triticum aestivum</i> L.) Plants leaf rust yellow rust wheat resistance GWAS breeding |
| title | A GBS-Based GWAS Analysis of Leaf and Stripe Rust Resistance in Diverse Pre-Breeding Germplasm of Bread Wheat (<i>Triticum aestivum</i> L.) |
| title_full | A GBS-Based GWAS Analysis of Leaf and Stripe Rust Resistance in Diverse Pre-Breeding Germplasm of Bread Wheat (<i>Triticum aestivum</i> L.) |
| title_fullStr | A GBS-Based GWAS Analysis of Leaf and Stripe Rust Resistance in Diverse Pre-Breeding Germplasm of Bread Wheat (<i>Triticum aestivum</i> L.) |
| title_full_unstemmed | A GBS-Based GWAS Analysis of Leaf and Stripe Rust Resistance in Diverse Pre-Breeding Germplasm of Bread Wheat (<i>Triticum aestivum</i> L.) |
| title_short | A GBS-Based GWAS Analysis of Leaf and Stripe Rust Resistance in Diverse Pre-Breeding Germplasm of Bread Wheat (<i>Triticum aestivum</i> L.) |
| title_sort | gbs based gwas analysis of leaf and stripe rust resistance in diverse pre breeding germplasm of bread wheat i triticum aestivum i l |
| topic | leaf rust yellow rust wheat resistance GWAS breeding |
| url | https://www.mdpi.com/2223-7747/11/18/2363 |
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