Impacts of engineered nanoparticles and antibiotics on denitrification: Element cycling functional genes and antibiotic resistance genes
The wide presence of antibiotics and minerals warrants their combined effects on the denitrification in natural aquatic environment. Herein, we investigated the effects of two antibiotics, sulfamethazine (SMZ) and chlortetracycline (CTC), on the reduction of NO3--N and accumulation of NO2--N in the...
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| Format: | Article |
| Language: | English |
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Elsevier
2022-08-01
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| Series: | Ecotoxicology and Environmental Safety |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S0147651322006273 |
| _version_ | 1818532807200210944 |
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| author | Ruijie Zhang Xuming Xu Yitao Lyu Ying Zhou Qian Chen Weiling Sun |
| author_facet | Ruijie Zhang Xuming Xu Yitao Lyu Ying Zhou Qian Chen Weiling Sun |
| author_sort | Ruijie Zhang |
| collection | DOAJ |
| description | The wide presence of antibiotics and minerals warrants their combined effects on the denitrification in natural aquatic environment. Herein, we investigated the effects of two antibiotics, sulfamethazine (SMZ) and chlortetracycline (CTC), on the reduction of NO3--N and accumulation of NO2--N in the absence and presence of engineered nanoparticles (NPs) (Al2O3, SiO2, and geothite) using 16 S rRNA sequencing and high-throughput quantitative PCR. The results showed that the addition of antibiotics inhibited the reduction of NO3--N by changing the bacterial community structure and reducing the abundance of denitrification genes, while engineered NPs promoted the denitrification by increasing the abundance of denitrification genes. In the binary systems, engineered NPs alleviated the inhibitory effect of antibiotics through enriching the denitrification genes and adsorbing antibiotics. Antibiotics and its combination with engineered NPs changed the composition of functional genes related to C, N, P, S metabolisms (p < 0.01). The addition of antibiotics and/or engineered NPs altered the bacterial community structure, which is dominated by the genera of Enterobacter (40.7–90.5%), Bacillus (4.9–58.5%), and Pseudomonas (0.21–12.7%). The significant relationship between denitrification, carbon metabolism genes, and antibiotic resistance genes revealed that the heterotrophic denitrifying bacteria may host the antibiotic resistance genes and denitrification genes simultaneously. The findings underscore the significance of engineered NPs in the toxicity assessment of pollutants, and provide a more realistic insight into the toxicity of antibiotics in the natural aquatic environment. |
| first_indexed | 2024-12-11T17:50:20Z |
| format | Article |
| id | doaj.art-bb36d4cb3529457ca707ceadd498d99e |
| institution | Directory Open Access Journal |
| issn | 0147-6513 |
| language | English |
| last_indexed | 2024-12-11T17:50:20Z |
| publishDate | 2022-08-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Ecotoxicology and Environmental Safety |
| spelling | doaj.art-bb36d4cb3529457ca707ceadd498d99e2022-12-22T00:56:15ZengElsevierEcotoxicology and Environmental Safety0147-65132022-08-01241113787Impacts of engineered nanoparticles and antibiotics on denitrification: Element cycling functional genes and antibiotic resistance genesRuijie Zhang0Xuming Xu1Yitao Lyu2Ying Zhou3Qian Chen4Weiling Sun5College of Environmental Sciences and Engineering, Peking University, Beijing 100871, China; International Joint Laboratory for Regional Pollution Control, Ministry of Education, Beijing 100871, China; State Environmental Protection Key Laboratory of All Material Fluxes in River Ecosystems, Beijing 100871, ChinaCollege of Environmental Sciences and Engineering, Peking University, Beijing 100871, China; International Joint Laboratory for Regional Pollution Control, Ministry of Education, Beijing 100871, China; State Environmental Protection Key Laboratory of All Material Fluxes in River Ecosystems, Beijing 100871, ChinaCollege of Environmental Sciences and Engineering, Peking University, Beijing 100871, China; International Joint Laboratory for Regional Pollution Control, Ministry of Education, Beijing 100871, China; State Environmental Protection Key Laboratory of All Material Fluxes in River Ecosystems, Beijing 100871, China; The Key Laboratory of Water and Sediment Sciences, Ministry of Education, Beijing 100871, ChinaCollege of Environmental Sciences and Engineering, Peking University, Beijing 100871, China; International Joint Laboratory for Regional Pollution Control, Ministry of Education, Beijing 100871, China; State Environmental Protection Key Laboratory of All Material Fluxes in River Ecosystems, Beijing 100871, China; The Key Laboratory of Water and Sediment Sciences, Ministry of Education, Beijing 100871, ChinaCollege of Environmental Sciences and Engineering, Peking University, Beijing 100871, China; International Joint Laboratory for Regional Pollution Control, Ministry of Education, Beijing 100871, China; State Environmental Protection Key Laboratory of All Material Fluxes in River Ecosystems, Beijing 100871, China; The Key Laboratory of Water and Sediment Sciences, Ministry of Education, Beijing 100871, ChinaCollege of Environmental Sciences and Engineering, Peking University, Beijing 100871, China; International Joint Laboratory for Regional Pollution Control, Ministry of Education, Beijing 100871, China; State Environmental Protection Key Laboratory of All Material Fluxes in River Ecosystems, Beijing 100871, China; The Key Laboratory of Water and Sediment Sciences, Ministry of Education, Beijing 100871, China; Correspondence to: College of Environmental Sciences and Engineering, Peking University, The Key Laboratory of Water and Sediment Sciences, Ministry of Education, Beijing 100871, China.The wide presence of antibiotics and minerals warrants their combined effects on the denitrification in natural aquatic environment. Herein, we investigated the effects of two antibiotics, sulfamethazine (SMZ) and chlortetracycline (CTC), on the reduction of NO3--N and accumulation of NO2--N in the absence and presence of engineered nanoparticles (NPs) (Al2O3, SiO2, and geothite) using 16 S rRNA sequencing and high-throughput quantitative PCR. The results showed that the addition of antibiotics inhibited the reduction of NO3--N by changing the bacterial community structure and reducing the abundance of denitrification genes, while engineered NPs promoted the denitrification by increasing the abundance of denitrification genes. In the binary systems, engineered NPs alleviated the inhibitory effect of antibiotics through enriching the denitrification genes and adsorbing antibiotics. Antibiotics and its combination with engineered NPs changed the composition of functional genes related to C, N, P, S metabolisms (p < 0.01). The addition of antibiotics and/or engineered NPs altered the bacterial community structure, which is dominated by the genera of Enterobacter (40.7–90.5%), Bacillus (4.9–58.5%), and Pseudomonas (0.21–12.7%). The significant relationship between denitrification, carbon metabolism genes, and antibiotic resistance genes revealed that the heterotrophic denitrifying bacteria may host the antibiotic resistance genes and denitrification genes simultaneously. The findings underscore the significance of engineered NPs in the toxicity assessment of pollutants, and provide a more realistic insight into the toxicity of antibiotics in the natural aquatic environment.http://www.sciencedirect.com/science/article/pii/S0147651322006273Engineered nanoparticlesAntibioticDenitrificationAntibiotic resistance gene |
| spellingShingle | Ruijie Zhang Xuming Xu Yitao Lyu Ying Zhou Qian Chen Weiling Sun Impacts of engineered nanoparticles and antibiotics on denitrification: Element cycling functional genes and antibiotic resistance genes Ecotoxicology and Environmental Safety Engineered nanoparticles Antibiotic Denitrification Antibiotic resistance gene |
| title | Impacts of engineered nanoparticles and antibiotics on denitrification: Element cycling functional genes and antibiotic resistance genes |
| title_full | Impacts of engineered nanoparticles and antibiotics on denitrification: Element cycling functional genes and antibiotic resistance genes |
| title_fullStr | Impacts of engineered nanoparticles and antibiotics on denitrification: Element cycling functional genes and antibiotic resistance genes |
| title_full_unstemmed | Impacts of engineered nanoparticles and antibiotics on denitrification: Element cycling functional genes and antibiotic resistance genes |
| title_short | Impacts of engineered nanoparticles and antibiotics on denitrification: Element cycling functional genes and antibiotic resistance genes |
| title_sort | impacts of engineered nanoparticles and antibiotics on denitrification element cycling functional genes and antibiotic resistance genes |
| topic | Engineered nanoparticles Antibiotic Denitrification Antibiotic resistance gene |
| url | http://www.sciencedirect.com/science/article/pii/S0147651322006273 |
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