Circ-SAR1A Promotes Renal Cell Carcinoma Progression Through miR-382/YBX1 Axis
Xiaolei Zhao,* Zhenhua Zhao,* Wenchao Xu, Hui Liu, Junkai Chang, Weibo Xu, Song Li, Songqiang Cao, Junqing Hou Department of Urology, Huaihe Hospital of Henan University, Kaifeng 475000, People’s Republic of China*These authors contributed equally to this workCorrespondence: Junqing HouDep...
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| Format: | Article |
| Language: | English |
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Dove Medical Press
2020-08-01
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| Series: | Cancer Management and Research |
| Subjects: | |
| Online Access: | https://www.dovepress.com/circ-sar1a-promotes-renal-cell-carcinoma-progression-through-mir-382yb-peer-reviewed-article-CMAR |
| _version_ | 1819016000484409344 |
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| author | Zhao X Zhao Z Xu W Liu H Chang J Xu W Li S Cao S Hou J |
| author_facet | Zhao X Zhao Z Xu W Liu H Chang J Xu W Li S Cao S Hou J |
| author_sort | Zhao X |
| collection | DOAJ |
| description | Xiaolei Zhao,* Zhenhua Zhao,* Wenchao Xu, Hui Liu, Junkai Chang, Weibo Xu, Song Li, Songqiang Cao, Junqing Hou Department of Urology, Huaihe Hospital of Henan University, Kaifeng 475000, People’s Republic of China*These authors contributed equally to this workCorrespondence: Junqing HouDepartment of Urology, Huaihe Hospital of Henan University, Kaifeng 475000, People’s Republic of ChinaEmail hjq0418@sina.comBackground: Accumulating evidence points to a role for circular RNAs (circRNAs) in important regulatory function in tumor advancement. We explored the effect and function of circ-SAR1A in renal cell carcinoma (RCC).Methods: circ-SAR1A expression in RCC tissues and cell lines was explored by qRT-PCR. The roles of circ-SAR1A on RCC progression were explored by in vitro function assays. Moreover, we determined the underlying mechanism of circ-SAR1A in RCC progression through bioinformatics analysis and dual-luciferase reporter assays.Results: Our data reveal that circ-SAR1A is significantly high in RCC tissues and cell lines. High circ-SAR1A levels are correlated to advanced Fuhrman grade, and lymph-node metastasis in RCC patients. Functional experiments indicate that circ-SAR1A suppression decreased RCC cell growth and invasion abilities in vitro. Mechanistically, circ-SAR1A upregulated YBX1 expression by sponging miR-382, resulting in promoting the growth and invasion in RCC cells.Conclusion: Our data indicate that the circ-SAR1A/miR-382/YBX1 axis plays a critical role in RCC progression, which serve as a potential novel treatment strategy of RCC.Keywords: circ-SAR1A, miR-382, YBX1, renal cell carcinoma |
| first_indexed | 2024-12-21T02:40:40Z |
| format | Article |
| id | doaj.art-bb388fa5e169470fad5ada7f0f181f4d |
| institution | Directory Open Access Journal |
| issn | 1179-1322 |
| language | English |
| last_indexed | 2024-12-21T02:40:40Z |
| publishDate | 2020-08-01 |
| publisher | Dove Medical Press |
| record_format | Article |
| series | Cancer Management and Research |
| spelling | doaj.art-bb388fa5e169470fad5ada7f0f181f4d2022-12-21T19:18:41ZengDove Medical PressCancer Management and Research1179-13222020-08-01Volume 127353736156330Circ-SAR1A Promotes Renal Cell Carcinoma Progression Through miR-382/YBX1 AxisZhao XZhao ZXu WLiu HChang JXu WLi SCao SHou JXiaolei Zhao,* Zhenhua Zhao,* Wenchao Xu, Hui Liu, Junkai Chang, Weibo Xu, Song Li, Songqiang Cao, Junqing Hou Department of Urology, Huaihe Hospital of Henan University, Kaifeng 475000, People’s Republic of China*These authors contributed equally to this workCorrespondence: Junqing HouDepartment of Urology, Huaihe Hospital of Henan University, Kaifeng 475000, People’s Republic of ChinaEmail hjq0418@sina.comBackground: Accumulating evidence points to a role for circular RNAs (circRNAs) in important regulatory function in tumor advancement. We explored the effect and function of circ-SAR1A in renal cell carcinoma (RCC).Methods: circ-SAR1A expression in RCC tissues and cell lines was explored by qRT-PCR. The roles of circ-SAR1A on RCC progression were explored by in vitro function assays. Moreover, we determined the underlying mechanism of circ-SAR1A in RCC progression through bioinformatics analysis and dual-luciferase reporter assays.Results: Our data reveal that circ-SAR1A is significantly high in RCC tissues and cell lines. High circ-SAR1A levels are correlated to advanced Fuhrman grade, and lymph-node metastasis in RCC patients. Functional experiments indicate that circ-SAR1A suppression decreased RCC cell growth and invasion abilities in vitro. Mechanistically, circ-SAR1A upregulated YBX1 expression by sponging miR-382, resulting in promoting the growth and invasion in RCC cells.Conclusion: Our data indicate that the circ-SAR1A/miR-382/YBX1 axis plays a critical role in RCC progression, which serve as a potential novel treatment strategy of RCC.Keywords: circ-SAR1A, miR-382, YBX1, renal cell carcinomahttps://www.dovepress.com/circ-sar1a-promotes-renal-cell-carcinoma-progression-through-mir-382yb-peer-reviewed-article-CMARcirc-sar1amir-382ybx1renal cell carcinoma |
| spellingShingle | Zhao X Zhao Z Xu W Liu H Chang J Xu W Li S Cao S Hou J Circ-SAR1A Promotes Renal Cell Carcinoma Progression Through miR-382/YBX1 Axis Cancer Management and Research circ-sar1a mir-382 ybx1 renal cell carcinoma |
| title | Circ-SAR1A Promotes Renal Cell Carcinoma Progression Through miR-382/YBX1 Axis |
| title_full | Circ-SAR1A Promotes Renal Cell Carcinoma Progression Through miR-382/YBX1 Axis |
| title_fullStr | Circ-SAR1A Promotes Renal Cell Carcinoma Progression Through miR-382/YBX1 Axis |
| title_full_unstemmed | Circ-SAR1A Promotes Renal Cell Carcinoma Progression Through miR-382/YBX1 Axis |
| title_short | Circ-SAR1A Promotes Renal Cell Carcinoma Progression Through miR-382/YBX1 Axis |
| title_sort | circ sar1a promotes renal cell carcinoma progression through mir 382 ybx1 axis |
| topic | circ-sar1a mir-382 ybx1 renal cell carcinoma |
| url | https://www.dovepress.com/circ-sar1a-promotes-renal-cell-carcinoma-progression-through-mir-382yb-peer-reviewed-article-CMAR |
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