Expression of Transcription Factor FOXO3a is Decreased in Patients with Ulcerative Colitis
Background: Ulcerative colitis (UC) is associated with differential expression of genes involved in inflammation and tissue remodeling, including FOXO3a, which encodes a transcription factor known to promote inflammation in several tissues. However, FOXO3a expression in tissues affected by UC has no...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Wolters Kluwer
2015-01-01
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| Series: | Chinese Medical Journal |
| Subjects: | |
| Online Access: | http://www.cmj.org/article.asp?issn=0366-6999;year=2015;volume=128;issue=20;spage=2759;epage=2763;aulast=Min |
| _version_ | 1819037312872349696 |
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| author | Min Min Jing Yang Yun-Sheng Yang Yan Liu Li-Mei Liu Yang Xu |
| author_facet | Min Min Jing Yang Yun-Sheng Yang Yan Liu Li-Mei Liu Yang Xu |
| author_sort | Min Min |
| collection | DOAJ |
| description | Background: Ulcerative colitis (UC) is associated with differential expression of genes involved in inflammation and tissue remodeling, including FOXO3a, which encodes a transcription factor known to promote inflammation in several tissues. However, FOXO3a expression in tissues affected by UC has not been examined. This study investigated the effects of FOXO3a on UC pathogenesis.
Methods: FOXO3a expression, in 23 patients with UC and in HT29 cells treated with tumor necrosis factor-α (TNF-α) for various durations, was detected by quantitative real-time polymerase chain reaction and Western blotting analysis. Enzyme-linked immunosorbent assay was used to quantify interleukin (IL)-8 expression in FOXO3a-silenced HT29 cells treated with TNF-α for various durations.
Results: The messenger RNA and protein expression of FOXO3a were significantly lower in UC tissues than those in normal subjects (P < 0.01). TNF-α treatment for 0, 0.5, 1, 6, and 24 h induced FOXO3 degradation in HT29 cells. FOXO3a silencing increased IL-8 levels in HT29 cells treated with TNF-α for 6 h (P < 0.05).
Conclusion: FOXO3a may play an important role in the intestinal inflammation of patients with UC. |
| first_indexed | 2024-12-21T08:19:25Z |
| format | Article |
| id | doaj.art-bb389955ba1b4a0391455eea8d1f6b77 |
| institution | Directory Open Access Journal |
| issn | 0366-6999 |
| language | English |
| last_indexed | 2024-12-21T08:19:25Z |
| publishDate | 2015-01-01 |
| publisher | Wolters Kluwer |
| record_format | Article |
| series | Chinese Medical Journal |
| spelling | doaj.art-bb389955ba1b4a0391455eea8d1f6b772022-12-21T19:10:28ZengWolters KluwerChinese Medical Journal0366-69992015-01-01128202759276310.4103/0366-6999.167314Expression of Transcription Factor FOXO3a is Decreased in Patients with Ulcerative ColitisMin MinJing YangYun-Sheng YangYan LiuLi-Mei LiuYang XuBackground: Ulcerative colitis (UC) is associated with differential expression of genes involved in inflammation and tissue remodeling, including FOXO3a, which encodes a transcription factor known to promote inflammation in several tissues. However, FOXO3a expression in tissues affected by UC has not been examined. This study investigated the effects of FOXO3a on UC pathogenesis. Methods: FOXO3a expression, in 23 patients with UC and in HT29 cells treated with tumor necrosis factor-α (TNF-α) for various durations, was detected by quantitative real-time polymerase chain reaction and Western blotting analysis. Enzyme-linked immunosorbent assay was used to quantify interleukin (IL)-8 expression in FOXO3a-silenced HT29 cells treated with TNF-α for various durations. Results: The messenger RNA and protein expression of FOXO3a were significantly lower in UC tissues than those in normal subjects (P < 0.01). TNF-α treatment for 0, 0.5, 1, 6, and 24 h induced FOXO3 degradation in HT29 cells. FOXO3a silencing increased IL-8 levels in HT29 cells treated with TNF-α for 6 h (P < 0.05). Conclusion: FOXO3a may play an important role in the intestinal inflammation of patients with UC.http://www.cmj.org/article.asp?issn=0366-6999;year=2015;volume=128;issue=20;spage=2759;epage=2763;aulast=MinFOXO3a; Interleukin-8; Ulcerative Colitis |
| spellingShingle | Min Min Jing Yang Yun-Sheng Yang Yan Liu Li-Mei Liu Yang Xu Expression of Transcription Factor FOXO3a is Decreased in Patients with Ulcerative Colitis Chinese Medical Journal FOXO3a; Interleukin-8; Ulcerative Colitis |
| title | Expression of Transcription Factor FOXO3a is Decreased in Patients with Ulcerative Colitis |
| title_full | Expression of Transcription Factor FOXO3a is Decreased in Patients with Ulcerative Colitis |
| title_fullStr | Expression of Transcription Factor FOXO3a is Decreased in Patients with Ulcerative Colitis |
| title_full_unstemmed | Expression of Transcription Factor FOXO3a is Decreased in Patients with Ulcerative Colitis |
| title_short | Expression of Transcription Factor FOXO3a is Decreased in Patients with Ulcerative Colitis |
| title_sort | expression of transcription factor foxo3a is decreased in patients with ulcerative colitis |
| topic | FOXO3a; Interleukin-8; Ulcerative Colitis |
| url | http://www.cmj.org/article.asp?issn=0366-6999;year=2015;volume=128;issue=20;spage=2759;epage=2763;aulast=Min |
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