Bioengineering of Hair Follicle-like Structure for Validation of Hair Growth Promoting Compounds
We aimed to establish screening and efficacy test techniques for use in the development of hair-promoting agents. To this end, we used the dermal papilla cell (DPc)-derived immortalized cell line (SV40T-hTERT DPc) and neonatal foreskin-derived keratinocyte cell line (Ker-CT) to form an immortalized...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-11-01
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| Series: | Bioengineering |
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| Online Access: | https://www.mdpi.com/2306-5354/9/11/645 |
| _version_ | 1827647245779992576 |
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| author | Hyun Woo Joo Min Kyu Kim Soon Sun Bak Young Kwan Sung |
| author_facet | Hyun Woo Joo Min Kyu Kim Soon Sun Bak Young Kwan Sung |
| author_sort | Hyun Woo Joo |
| collection | DOAJ |
| description | We aimed to establish screening and efficacy test techniques for use in the development of hair-promoting agents. To this end, we used the dermal papilla cell (DPc)-derived immortalized cell line (SV40T-hTERT DPc) and neonatal foreskin-derived keratinocyte cell line (Ker-CT) to form an immortalized cell-based hair follicle-like structure. The SV40T-hTERT DPc spheroids exhibited a higher cell ratio in the spheroids than primary DPc spheroids, and SV40T-hTERT DPc aggregated with spheroids larger in diameter than primary DPc when the same cell number was seeded into the low-adhesion plate. Microscopic imaging and fluorescence staining results indicated that both primary and immortalized cell combinations form a hair follicle-like structure with a long-stretched keratinocyte layer under the condition that the spheroids have the same diameter as that of in vivo dermal papillary tissue in the hair follicle. The hair follicle-like structure elongation was increased upon treatment with three known hair follicle growth-promoting compounds (minoxidil, tofacitinib, and ascorbic acid) compared with that in the control group. Therefore, using immortalized cells to generate a coherent follicle-like structure, we have developed models for screening and evaluating hair-care materials commonly used in the industry. |
| first_indexed | 2024-03-09T19:16:29Z |
| format | Article |
| id | doaj.art-bb3b7cbea8ec44adb25b30bb26f4eca8 |
| institution | Directory Open Access Journal |
| issn | 2306-5354 |
| language | English |
| last_indexed | 2024-03-09T19:16:29Z |
| publishDate | 2022-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Bioengineering |
| spelling | doaj.art-bb3b7cbea8ec44adb25b30bb26f4eca82023-11-24T03:46:39ZengMDPI AGBioengineering2306-53542022-11-0191164510.3390/bioengineering9110645Bioengineering of Hair Follicle-like Structure for Validation of Hair Growth Promoting CompoundsHyun Woo Joo0Min Kyu Kim1Soon Sun Bak2Young Kwan Sung3Department of Immunology, School of Medicine, Kyungpook National University, Daegu 41944, KoreaDepartment of Immunology, School of Medicine, Kyungpook National University, Daegu 41944, KoreaDepartment of Immunology, School of Medicine, Kyungpook National University, Daegu 41944, KoreaDepartment of Immunology, School of Medicine, Kyungpook National University, Daegu 41944, KoreaWe aimed to establish screening and efficacy test techniques for use in the development of hair-promoting agents. To this end, we used the dermal papilla cell (DPc)-derived immortalized cell line (SV40T-hTERT DPc) and neonatal foreskin-derived keratinocyte cell line (Ker-CT) to form an immortalized cell-based hair follicle-like structure. The SV40T-hTERT DPc spheroids exhibited a higher cell ratio in the spheroids than primary DPc spheroids, and SV40T-hTERT DPc aggregated with spheroids larger in diameter than primary DPc when the same cell number was seeded into the low-adhesion plate. Microscopic imaging and fluorescence staining results indicated that both primary and immortalized cell combinations form a hair follicle-like structure with a long-stretched keratinocyte layer under the condition that the spheroids have the same diameter as that of in vivo dermal papillary tissue in the hair follicle. The hair follicle-like structure elongation was increased upon treatment with three known hair follicle growth-promoting compounds (minoxidil, tofacitinib, and ascorbic acid) compared with that in the control group. Therefore, using immortalized cells to generate a coherent follicle-like structure, we have developed models for screening and evaluating hair-care materials commonly used in the industry.https://www.mdpi.com/2306-5354/9/11/645alopeciahair follicleshair losskeratinocytesscreeningspheroids |
| spellingShingle | Hyun Woo Joo Min Kyu Kim Soon Sun Bak Young Kwan Sung Bioengineering of Hair Follicle-like Structure for Validation of Hair Growth Promoting Compounds Bioengineering alopecia hair follicles hair loss keratinocytes screening spheroids |
| title | Bioengineering of Hair Follicle-like Structure for Validation of Hair Growth Promoting Compounds |
| title_full | Bioengineering of Hair Follicle-like Structure for Validation of Hair Growth Promoting Compounds |
| title_fullStr | Bioengineering of Hair Follicle-like Structure for Validation of Hair Growth Promoting Compounds |
| title_full_unstemmed | Bioengineering of Hair Follicle-like Structure for Validation of Hair Growth Promoting Compounds |
| title_short | Bioengineering of Hair Follicle-like Structure for Validation of Hair Growth Promoting Compounds |
| title_sort | bioengineering of hair follicle like structure for validation of hair growth promoting compounds |
| topic | alopecia hair follicles hair loss keratinocytes screening spheroids |
| url | https://www.mdpi.com/2306-5354/9/11/645 |
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