Gastroprotective effects of Polygonatum odoratum in rodents by regulation of apoptotic proteins and inflammatory cytokines
In an increasing interest in natural antiulcer compounds that may have gastric healing effects and possibly prevent ulcer recurrence, Polygonatum odoratum appears as a strong candidate. The gastroprotective potentials of P. odoratum rhizome extract (PORE) were explored on ethanol-induced gastric ulc...
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| Format: | Article |
| Language: | English |
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Elsevier
2023-06-01
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| Series: | Saudi Journal of Biological Sciences |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S1319562X23001237 |
| _version_ | 1797817519116910592 |
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| author | Abdalbasit A. Mariod Ahmed A.J. Jabbar Zaenah Zuhair Alamri Ahmed Salim Al Rashdi Mahmood Ameen Abdulla |
| author_facet | Abdalbasit A. Mariod Ahmed A.J. Jabbar Zaenah Zuhair Alamri Ahmed Salim Al Rashdi Mahmood Ameen Abdulla |
| author_sort | Abdalbasit A. Mariod |
| collection | DOAJ |
| description | In an increasing interest in natural antiulcer compounds that may have gastric healing effects and possibly prevent ulcer recurrence, Polygonatum odoratum appears as a strong candidate. The gastroprotective potentials of P. odoratum rhizome extract (PORE) were explored on ethanol-induced gastric ulceration in rats. Sprague Dawley rats were caged in 5 groups, normal and ulcer control rats received CMC (1% carboxymethyl cellulose). Omeprazole (20 mg/kg) was given to reference Rats. Experimental rats were treated with 250 mg/kg and 500 mg/kg PORE, respectively. After an hour, the normal control rats received 1% CMC, whereas rat groups 2–5 were given absolute ethanol by oral gavage. After 60 min, rats received anesthesia and were sacrificed. Dissected gastric tissue was analyzed by histopathological and immunohistochemical techniques. PORE treatment significantly lowered the ethanol-induced gastric injury, as shown by up-surging gastric pH and mucus content, reduced leukocyte infiltration, lower ulcerative areas in mucosal layers, and increased antioxidants (SOD and CAT) and (MDA) levels. Furthermore, PORE pre-treated rats showed significantly increased expression of the Periodic acid-Schiff (PAS), HSP-70 protein, and decreased Bax protein in their gastric epithelial layers. PORE treatment showed an important regulation of inflammatory cytokines shown by decreasing the TNF-a, and IL-6 and increasing the IL-10 values. The detected biological activity of PORE is encouraging and presents the scientific evidence for its traditional use as a gastroprotection agent however further studies are required to determine the exact phytochemicals and mechanism pathway responsible for this bioactivity. |
| first_indexed | 2024-03-13T08:54:38Z |
| format | Article |
| id | doaj.art-bb3defd5a9f3453ca316da48d6f90646 |
| institution | Directory Open Access Journal |
| issn | 1319-562X |
| language | English |
| last_indexed | 2024-03-13T08:54:38Z |
| publishDate | 2023-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Saudi Journal of Biological Sciences |
| spelling | doaj.art-bb3defd5a9f3453ca316da48d6f906462023-05-29T04:03:52ZengElsevierSaudi Journal of Biological Sciences1319-562X2023-06-01306103678Gastroprotective effects of Polygonatum odoratum in rodents by regulation of apoptotic proteins and inflammatory cytokinesAbdalbasit A. Mariod0Ahmed A.J. Jabbar1Zaenah Zuhair Alamri2Ahmed Salim Al Rashdi3Mahmood Ameen Abdulla4College of Science and Arts, Alkamil Branch, University of Jeddah, Alkamil 21931, Saudi Arabia; Indigenous Knowledge and Heritage Centre, Ghibaish College of Science and Technology, 110 Ghibaish, SudanDepartment of Medical Laboratory Technology, Erbil Technical Health and Medical College, Erbil Polytechnic University, Erbil 44001, Iraq; Corresponding author.Department of Biological Sciences, Faculty of Science, University of Jeddah, Jeddah, Saudi ArabiaCentral Public Health Laboratories, Ministry of Health, Muscat 111, OmanDepartment of Medical Microbiology, College of Science, Cihan University-Erbil, Erbil 44001, Kurdistan Region, IraqIn an increasing interest in natural antiulcer compounds that may have gastric healing effects and possibly prevent ulcer recurrence, Polygonatum odoratum appears as a strong candidate. The gastroprotective potentials of P. odoratum rhizome extract (PORE) were explored on ethanol-induced gastric ulceration in rats. Sprague Dawley rats were caged in 5 groups, normal and ulcer control rats received CMC (1% carboxymethyl cellulose). Omeprazole (20 mg/kg) was given to reference Rats. Experimental rats were treated with 250 mg/kg and 500 mg/kg PORE, respectively. After an hour, the normal control rats received 1% CMC, whereas rat groups 2–5 were given absolute ethanol by oral gavage. After 60 min, rats received anesthesia and were sacrificed. Dissected gastric tissue was analyzed by histopathological and immunohistochemical techniques. PORE treatment significantly lowered the ethanol-induced gastric injury, as shown by up-surging gastric pH and mucus content, reduced leukocyte infiltration, lower ulcerative areas in mucosal layers, and increased antioxidants (SOD and CAT) and (MDA) levels. Furthermore, PORE pre-treated rats showed significantly increased expression of the Periodic acid-Schiff (PAS), HSP-70 protein, and decreased Bax protein in their gastric epithelial layers. PORE treatment showed an important regulation of inflammatory cytokines shown by decreasing the TNF-a, and IL-6 and increasing the IL-10 values. The detected biological activity of PORE is encouraging and presents the scientific evidence for its traditional use as a gastroprotection agent however further studies are required to determine the exact phytochemicals and mechanism pathway responsible for this bioactivity.http://www.sciencedirect.com/science/article/pii/S1319562X23001237Polygonatum odoratum rhizome extractOxidative stressStomach ulcerHistologyImmunohistochemistryAcute toxicity |
| spellingShingle | Abdalbasit A. Mariod Ahmed A.J. Jabbar Zaenah Zuhair Alamri Ahmed Salim Al Rashdi Mahmood Ameen Abdulla Gastroprotective effects of Polygonatum odoratum in rodents by regulation of apoptotic proteins and inflammatory cytokines Saudi Journal of Biological Sciences Polygonatum odoratum rhizome extract Oxidative stress Stomach ulcer Histology Immunohistochemistry Acute toxicity |
| title | Gastroprotective effects of Polygonatum odoratum in rodents by regulation of apoptotic proteins and inflammatory cytokines |
| title_full | Gastroprotective effects of Polygonatum odoratum in rodents by regulation of apoptotic proteins and inflammatory cytokines |
| title_fullStr | Gastroprotective effects of Polygonatum odoratum in rodents by regulation of apoptotic proteins and inflammatory cytokines |
| title_full_unstemmed | Gastroprotective effects of Polygonatum odoratum in rodents by regulation of apoptotic proteins and inflammatory cytokines |
| title_short | Gastroprotective effects of Polygonatum odoratum in rodents by regulation of apoptotic proteins and inflammatory cytokines |
| title_sort | gastroprotective effects of polygonatum odoratum in rodents by regulation of apoptotic proteins and inflammatory cytokines |
| topic | Polygonatum odoratum rhizome extract Oxidative stress Stomach ulcer Histology Immunohistochemistry Acute toxicity |
| url | http://www.sciencedirect.com/science/article/pii/S1319562X23001237 |
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