Proteomic Profiling of Sugar Beet (Beta vulgaris) Leaves during Rhizomania Compatible Interactions
Rhizomania, caused by Beet necrotic yellow vein virus (BNYVV), severely impacts sugar beet (Beta vulgaris) production throughout the world, and is widely prevalent in most production regions. Initial efforts to characterize proteome changes focused primarily on identifying putative host factors that...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2014-04-01
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| Series: | Proteomes |
| Subjects: | |
| Online Access: | http://www.mdpi.com/2227-7382/2/2/208 |
| _version_ | 1811305911165124608 |
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| author | Kimberly M. Webb Carolyn J. Broccardo Jessica E. Prenni William M. Wintermantel |
| author_facet | Kimberly M. Webb Carolyn J. Broccardo Jessica E. Prenni William M. Wintermantel |
| author_sort | Kimberly M. Webb |
| collection | DOAJ |
| description | Rhizomania, caused by Beet necrotic yellow vein virus (BNYVV), severely impacts sugar beet (Beta vulgaris) production throughout the world, and is widely prevalent in most production regions. Initial efforts to characterize proteome changes focused primarily on identifying putative host factors that elicit resistant interactions with BNYVV, but as resistance breaking strains become more prevalent, effective disease control strategies will require the application of novel methods based on better understanding of disease susceptibility and symptom development. Herein, proteomic profiling was conducted on susceptible sugar beet, infected with two strains of BNYVV, to clarify the types of proteins prevalent during compatible virus-host plant interactions. Total protein was extracted from sugar beet leaf tissue infected with BNYVV, quantified, and analyzed by mass spectrometry. A total of 203 proteins were confidently identified, with a predominance of proteins associated with photosynthesis and energy, metabolism, and response to stimulus. Many proteins identified in this study are typically associated with systemic acquired resistance and general plant defense responses. These results expand on relatively limited proteomic data available for sugar beet and provide the ground work for additional studies focused on understanding the interaction of BNYVV with sugar beet. |
| first_indexed | 2024-04-13T08:34:53Z |
| format | Article |
| id | doaj.art-bb41bd6c778b476382fb70218f691646 |
| institution | Directory Open Access Journal |
| issn | 2227-7382 |
| language | English |
| last_indexed | 2024-04-13T08:34:53Z |
| publishDate | 2014-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Proteomes |
| spelling | doaj.art-bb41bd6c778b476382fb70218f6916462022-12-22T02:54:09ZengMDPI AGProteomes2227-73822014-04-012220822310.3390/proteomes2020208proteomes2020208Proteomic Profiling of Sugar Beet (Beta vulgaris) Leaves during Rhizomania Compatible InteractionsKimberly M. Webb0Carolyn J. Broccardo1Jessica E. Prenni2William M. Wintermantel3USDA-ARS-SBRU, Crops Research Laboratory, 1701 Centre Ave., Fort Collins, CO 80526, USAProteomics and Metabolomics Facility, Colorado State University, C130 Microbiology, 2021 Campus Delivery, Fort Collins, CO 80523, USAProteomics and Metabolomics Facility, Colorado State University, C130 Microbiology, 2021 Campus Delivery, Fort Collins, CO 80523, USAUSDA-ARS-CIPRU, 1636 E. Alisal St., Salinas, CA 93905, USARhizomania, caused by Beet necrotic yellow vein virus (BNYVV), severely impacts sugar beet (Beta vulgaris) production throughout the world, and is widely prevalent in most production regions. Initial efforts to characterize proteome changes focused primarily on identifying putative host factors that elicit resistant interactions with BNYVV, but as resistance breaking strains become more prevalent, effective disease control strategies will require the application of novel methods based on better understanding of disease susceptibility and symptom development. Herein, proteomic profiling was conducted on susceptible sugar beet, infected with two strains of BNYVV, to clarify the types of proteins prevalent during compatible virus-host plant interactions. Total protein was extracted from sugar beet leaf tissue infected with BNYVV, quantified, and analyzed by mass spectrometry. A total of 203 proteins were confidently identified, with a predominance of proteins associated with photosynthesis and energy, metabolism, and response to stimulus. Many proteins identified in this study are typically associated with systemic acquired resistance and general plant defense responses. These results expand on relatively limited proteomic data available for sugar beet and provide the ground work for additional studies focused on understanding the interaction of BNYVV with sugar beet.http://www.mdpi.com/2227-7382/2/2/208Beet necrotic yellow vein virus (BNYVV) shot-gun proteomicssugarbeet |
| spellingShingle | Kimberly M. Webb Carolyn J. Broccardo Jessica E. Prenni William M. Wintermantel Proteomic Profiling of Sugar Beet (Beta vulgaris) Leaves during Rhizomania Compatible Interactions Proteomes Beet necrotic yellow vein virus (BNYVV) shot-gun proteomics sugarbeet |
| title | Proteomic Profiling of Sugar Beet (Beta vulgaris) Leaves during Rhizomania Compatible Interactions |
| title_full | Proteomic Profiling of Sugar Beet (Beta vulgaris) Leaves during Rhizomania Compatible Interactions |
| title_fullStr | Proteomic Profiling of Sugar Beet (Beta vulgaris) Leaves during Rhizomania Compatible Interactions |
| title_full_unstemmed | Proteomic Profiling of Sugar Beet (Beta vulgaris) Leaves during Rhizomania Compatible Interactions |
| title_short | Proteomic Profiling of Sugar Beet (Beta vulgaris) Leaves during Rhizomania Compatible Interactions |
| title_sort | proteomic profiling of sugar beet beta vulgaris leaves during rhizomania compatible interactions |
| topic | Beet necrotic yellow vein virus (BNYVV) shot-gun proteomics sugarbeet |
| url | http://www.mdpi.com/2227-7382/2/2/208 |
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