Embedded Immunodetection System for Fecal Occult Blood
In this paper, a rapid test system with high sensitivity, linearity, and stability is presented for fecal occult blood (FOB) detection. The coloration results of the immune response are used as the basis for the determination of the detection target in combination with an immunochromatographic strip...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2021-04-01
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| Series: | Biosensors |
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| Online Access: | https://www.mdpi.com/2079-6374/11/4/106 |
| _version_ | 1797538915706470400 |
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| author | Kai-Wen Lin Yu-Chi Chang |
| author_facet | Kai-Wen Lin Yu-Chi Chang |
| author_sort | Kai-Wen Lin |
| collection | DOAJ |
| description | In this paper, a rapid test system with high sensitivity, linearity, and stability is presented for fecal occult blood (FOB) detection. The coloration results of the immune response are used as the basis for the determination of the detection target in combination with an immunochromatographic strip. The rapid test system can be used to detect and calculate the concentration of the sample, so detection of the immune coloration response is more accurate in a quantitative analysis. The system is composed of both hardware and software. The programs used for the analysis and programmed by Python include the main program, polarization calibration, QR Code decoding, Bluetooth transmission, and image processing. After verification of each part of the system, it was found that the rapid test system successfully detects from 0 ng/mL to 400 ng/mL of FOB with coefficients of variation (CV) below 3.7% and 1000 ng/mL with a CV only at 7.41%. |
| first_indexed | 2024-03-10T12:37:57Z |
| format | Article |
| id | doaj.art-bb483323fb5841afa1b5fe5428777df6 |
| institution | Directory Open Access Journal |
| issn | 2079-6374 |
| language | English |
| last_indexed | 2024-03-10T12:37:57Z |
| publishDate | 2021-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biosensors |
| spelling | doaj.art-bb483323fb5841afa1b5fe5428777df62023-11-21T14:09:57ZengMDPI AGBiosensors2079-63742021-04-0111410610.3390/bios11040106Embedded Immunodetection System for Fecal Occult BloodKai-Wen Lin0Yu-Chi Chang1Department of Engineering Science, National Cheng Kung University, Tainan 701, TaiwanDepartment of Engineering Science, National Cheng Kung University, Tainan 701, TaiwanIn this paper, a rapid test system with high sensitivity, linearity, and stability is presented for fecal occult blood (FOB) detection. The coloration results of the immune response are used as the basis for the determination of the detection target in combination with an immunochromatographic strip. The rapid test system can be used to detect and calculate the concentration of the sample, so detection of the immune coloration response is more accurate in a quantitative analysis. The system is composed of both hardware and software. The programs used for the analysis and programmed by Python include the main program, polarization calibration, QR Code decoding, Bluetooth transmission, and image processing. After verification of each part of the system, it was found that the rapid test system successfully detects from 0 ng/mL to 400 ng/mL of FOB with coefficients of variation (CV) below 3.7% and 1000 ng/mL with a CV only at 7.41%.https://www.mdpi.com/2079-6374/11/4/106rapid testimmunodetection systemfecal occult blood |
| spellingShingle | Kai-Wen Lin Yu-Chi Chang Embedded Immunodetection System for Fecal Occult Blood Biosensors rapid test immunodetection system fecal occult blood |
| title | Embedded Immunodetection System for Fecal Occult Blood |
| title_full | Embedded Immunodetection System for Fecal Occult Blood |
| title_fullStr | Embedded Immunodetection System for Fecal Occult Blood |
| title_full_unstemmed | Embedded Immunodetection System for Fecal Occult Blood |
| title_short | Embedded Immunodetection System for Fecal Occult Blood |
| title_sort | embedded immunodetection system for fecal occult blood |
| topic | rapid test immunodetection system fecal occult blood |
| url | https://www.mdpi.com/2079-6374/11/4/106 |
| work_keys_str_mv | AT kaiwenlin embeddedimmunodetectionsystemforfecaloccultblood AT yuchichang embeddedimmunodetectionsystemforfecaloccultblood |