Spherical Amorphous Nanoparticles from Birch Bark Triterpenoids: Study of the Conditions for the Formation
Triterpenoids from birch bark are valuable biologically active substances. They exhibit important biological properties. However, triterpenoids are insoluble in water due to their hydrophobicity. In order to increase their bioavailability we developed a technique for obtaining spherical amorphous na...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | Russian |
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MIREA - Russian Technological University
2019-07-01
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| Series: | Тонкие химические технологии |
| Subjects: | |
| Online Access: | https://www.finechem-mirea.ru/jour/article/view/1270 |
| _version_ | 1797882064173793280 |
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| author | A. N. Bastrich A. E. Stepanov N. N. Lonina V. I. Popenko A. P. Kaplun |
| author_facet | A. N. Bastrich A. E. Stepanov N. N. Lonina V. I. Popenko A. P. Kaplun |
| author_sort | A. N. Bastrich |
| collection | DOAJ |
| description | Triterpenoids from birch bark are valuable biologically active substances. They exhibit important biological properties. However, triterpenoids are insoluble in water due to their hydrophobicity. In order to increase their bioavailability we developed a technique for obtaining spherical amorphous nanoparticles (SANP) by their precipitation with water from a solution in THF. In the form of nanoparticles, triterpenoids show additional useful properties: they can be loaded with hydrophobic substances. SANP were also shown to be effective immunological adjuvants. This article is devoted to the study of conditions that could optimize their production. As a result, it was shown that among the three investigated solvents miscible with water - acetone, dioxane, THF -the latter gives the best results. Lowering temperature decreases the size of SANP. An increased temperature leads to the formation of betulin crystals. When the concentration of triterpenoids in THF is higher than 5 mg/ml, crystals of betulin also begin to form. The results of this study lead to the assumption that the main parameter of the process determining the formation of SANP is the rate of the solvent diffusion into water. |
| first_indexed | 2024-04-10T03:29:00Z |
| format | Article |
| id | doaj.art-bb4a803c33f746159b35cc73940356d2 |
| institution | Directory Open Access Journal |
| issn | 2410-6593 2686-7575 |
| language | Russian |
| last_indexed | 2024-04-10T03:29:00Z |
| publishDate | 2019-07-01 |
| publisher | MIREA - Russian Technological University |
| record_format | Article |
| series | Тонкие химические технологии |
| spelling | doaj.art-bb4a803c33f746159b35cc73940356d22023-03-13T07:25:38ZrusMIREA - Russian Technological UniversityТонкие химические технологии2410-65932686-75752019-07-01143334110.32362/2410-6593-2019-14-3-33-411268Spherical Amorphous Nanoparticles from Birch Bark Triterpenoids: Study of the Conditions for the FormationA. N. Bastrich0A. E. Stepanov1N. N. Lonina2V. I. Popenko3A. P. Kaplun4MIREA - Russian Technological University (M.V. Lomonosov Institute of Fine Chemical Technologies)MIREA - Russian Technological University (M.V. Lomonosov Institute of Fine Chemical Technologies)MIREA - Russian Technological University (M.V. Lomonosov Institute of Fine Chemical Technologies)Engelhardt Institute of Molecular Biology, Russian Academy of SciencesMIREA - Russian Technological University (M.V. Lomonosov Institute of Fine Chemical Technologies)Triterpenoids from birch bark are valuable biologically active substances. They exhibit important biological properties. However, triterpenoids are insoluble in water due to their hydrophobicity. In order to increase their bioavailability we developed a technique for obtaining spherical amorphous nanoparticles (SANP) by their precipitation with water from a solution in THF. In the form of nanoparticles, triterpenoids show additional useful properties: they can be loaded with hydrophobic substances. SANP were also shown to be effective immunological adjuvants. This article is devoted to the study of conditions that could optimize their production. As a result, it was shown that among the three investigated solvents miscible with water - acetone, dioxane, THF -the latter gives the best results. Lowering temperature decreases the size of SANP. An increased temperature leads to the formation of betulin crystals. When the concentration of triterpenoids in THF is higher than 5 mg/ml, crystals of betulin also begin to form. The results of this study lead to the assumption that the main parameter of the process determining the formation of SANP is the rate of the solvent diffusion into water.https://www.finechem-mirea.ru/jour/article/view/1270birch bark triterpenoidsbetulinlupeolspherical amorphous nanoparticlesprecipitation by water from an organic solvent |
| spellingShingle | A. N. Bastrich A. E. Stepanov N. N. Lonina V. I. Popenko A. P. Kaplun Spherical Amorphous Nanoparticles from Birch Bark Triterpenoids: Study of the Conditions for the Formation Тонкие химические технологии birch bark triterpenoids betulin lupeol spherical amorphous nanoparticles precipitation by water from an organic solvent |
| title | Spherical Amorphous Nanoparticles from Birch Bark Triterpenoids: Study of the Conditions for the Formation |
| title_full | Spherical Amorphous Nanoparticles from Birch Bark Triterpenoids: Study of the Conditions for the Formation |
| title_fullStr | Spherical Amorphous Nanoparticles from Birch Bark Triterpenoids: Study of the Conditions for the Formation |
| title_full_unstemmed | Spherical Amorphous Nanoparticles from Birch Bark Triterpenoids: Study of the Conditions for the Formation |
| title_short | Spherical Amorphous Nanoparticles from Birch Bark Triterpenoids: Study of the Conditions for the Formation |
| title_sort | spherical amorphous nanoparticles from birch bark triterpenoids study of the conditions for the formation |
| topic | birch bark triterpenoids betulin lupeol spherical amorphous nanoparticles precipitation by water from an organic solvent |
| url | https://www.finechem-mirea.ru/jour/article/view/1270 |
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