Chenopodium ambrosioides L. essential oil and ethanol extract on control of canine Ancylostoma spp.
The use of Chenopodium ambrosioides L. has shown to be promising in the management of gastrointestinal nematodes. The objective of this study was to quantitate the yield and characterize the chemical composition of the essential oil of C. ambrosioides, as well as to evaluate the in vitro effect of t...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Universidade Estadual de Londrina
2017-08-01
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| Series: | Semina: Ciências Agrárias |
| Subjects: | |
| Online Access: | http://www.uel.br/revistas/uel/index.php/semagrarias/article/view/26470 |
| _version_ | 1818151172739956736 |
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| author | Jessica Nascimento Moraes Monteiro Anderson Barros Archanjo Gabriela Porfirio Passos Adilson Vidal Costa Lenir Cardoso Porfirio Isabella Vilhena Freire Martins |
| author_facet | Jessica Nascimento Moraes Monteiro Anderson Barros Archanjo Gabriela Porfirio Passos Adilson Vidal Costa Lenir Cardoso Porfirio Isabella Vilhena Freire Martins |
| author_sort | Jessica Nascimento Moraes Monteiro |
| collection | DOAJ |
| description | The use of Chenopodium ambrosioides L. has shown to be promising in the management of gastrointestinal nematodes. The objective of this study was to quantitate the yield and characterize the chemical composition of the essential oil of C. ambrosioides, as well as to evaluate the in vitro effect of the ethanolic extract and the essential oil in L3 of Ancylostoma spp. and the in vivo effect(s) of the essential oil in dogs. The effects of the ethanol extract and essential oil on Ancylostoma spp. were evaluated in vitro by exposing larvae to the extract at concentrations ranging from 0.005 g mL-1 to 0.2 g mL-1 and to essential oil at concentrations of 50, 100, and 150 ?L mL-1. For the in vivo test, 26 healthy dogs, naturally infected by Ancylostoma spp., were divided into three groups: F1 - cookies were administered without active principle; F2 - herbal cookies containing 37.5 ?L g-1 essential oil of C. ambrosioides L.; F3 - cookies plus a commercial formulation containing febantel, pyrantel, praziquantel, and ivermectin. Complete blood counts and serum biochemistry for AST, ALT, AF, urea, creatinine, total protein, and albumin were performed. The yield of the essential oil was 0.3% m v-1, and its major components included ?-terpinene (1.24%), p-cymene (4.83%), and ascaridol Z (87%) and E (5.04%) isomers. The concentrations of C. ambrosioides L. ethanol extract used were ineffective against Ancylostoma spp. larvae. The essential oil at a concentration of 150 ?L mL-1 was effective against L3 larvae. In the in vivo study in dogs, the herbal cookies containing C. ambrosioides L essential oil reduced the number of eggs per gram of feces. |
| first_indexed | 2024-12-11T13:34:36Z |
| format | Article |
| id | doaj.art-bb4b07173fb446249452467f6d7d5ecf |
| institution | Directory Open Access Journal |
| issn | 1676-546X 1679-0359 |
| language | English |
| last_indexed | 2024-12-11T13:34:36Z |
| publishDate | 2017-08-01 |
| publisher | Universidade Estadual de Londrina |
| record_format | Article |
| series | Semina: Ciências Agrárias |
| spelling | doaj.art-bb4b07173fb446249452467f6d7d5ecf2022-12-22T01:05:06ZengUniversidade Estadual de LondrinaSemina: Ciências Agrárias1676-546X1679-03592017-08-013841947195410.5433/1679-0359.2017v38n4p194715541Chenopodium ambrosioides L. essential oil and ethanol extract on control of canine Ancylostoma spp.Jessica Nascimento Moraes Monteiro0Anderson Barros Archanjo1Gabriela Porfirio Passos2Adilson Vidal Costa3Lenir Cardoso Porfirio4Isabella Vilhena Freire Martins5Universidade Federal do Espírito SantoUniversidade Federal do Espírito SantoUniversidade Federal da BahiaUniversidade Federal do Espírito SantoUniversidade Federal do Espírito SantoUniversidade Federal do Espírito SantoThe use of Chenopodium ambrosioides L. has shown to be promising in the management of gastrointestinal nematodes. The objective of this study was to quantitate the yield and characterize the chemical composition of the essential oil of C. ambrosioides, as well as to evaluate the in vitro effect of the ethanolic extract and the essential oil in L3 of Ancylostoma spp. and the in vivo effect(s) of the essential oil in dogs. The effects of the ethanol extract and essential oil on Ancylostoma spp. were evaluated in vitro by exposing larvae to the extract at concentrations ranging from 0.005 g mL-1 to 0.2 g mL-1 and to essential oil at concentrations of 50, 100, and 150 ?L mL-1. For the in vivo test, 26 healthy dogs, naturally infected by Ancylostoma spp., were divided into three groups: F1 - cookies were administered without active principle; F2 - herbal cookies containing 37.5 ?L g-1 essential oil of C. ambrosioides L.; F3 - cookies plus a commercial formulation containing febantel, pyrantel, praziquantel, and ivermectin. Complete blood counts and serum biochemistry for AST, ALT, AF, urea, creatinine, total protein, and albumin were performed. The yield of the essential oil was 0.3% m v-1, and its major components included ?-terpinene (1.24%), p-cymene (4.83%), and ascaridol Z (87%) and E (5.04%) isomers. The concentrations of C. ambrosioides L. ethanol extract used were ineffective against Ancylostoma spp. larvae. The essential oil at a concentration of 150 ?L mL-1 was effective against L3 larvae. In the in vivo study in dogs, the herbal cookies containing C. ambrosioides L essential oil reduced the number of eggs per gram of feces.http://www.uel.br/revistas/uel/index.php/semagrarias/article/view/26470Erva-de-santa-mariaHelmintosÓleo essencialPlanta medicinal. |
| spellingShingle | Jessica Nascimento Moraes Monteiro Anderson Barros Archanjo Gabriela Porfirio Passos Adilson Vidal Costa Lenir Cardoso Porfirio Isabella Vilhena Freire Martins Chenopodium ambrosioides L. essential oil and ethanol extract on control of canine Ancylostoma spp. Semina: Ciências Agrárias Erva-de-santa-maria Helmintos Óleo essencial Planta medicinal. |
| title | Chenopodium ambrosioides L. essential oil and ethanol extract on control of canine Ancylostoma spp. |
| title_full | Chenopodium ambrosioides L. essential oil and ethanol extract on control of canine Ancylostoma spp. |
| title_fullStr | Chenopodium ambrosioides L. essential oil and ethanol extract on control of canine Ancylostoma spp. |
| title_full_unstemmed | Chenopodium ambrosioides L. essential oil and ethanol extract on control of canine Ancylostoma spp. |
| title_short | Chenopodium ambrosioides L. essential oil and ethanol extract on control of canine Ancylostoma spp. |
| title_sort | chenopodium ambrosioides l essential oil and ethanol extract on control of canine ancylostoma spp |
| topic | Erva-de-santa-maria Helmintos Óleo essencial Planta medicinal. |
| url | http://www.uel.br/revistas/uel/index.php/semagrarias/article/view/26470 |
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