Rapid Identification of Chinese Hamster Ovary Cell Apoptosis and Its Potential Role in Process Robustness Assessment
Currently, the assessment of process robustness is often time-consuming, labor-intensive, and material-intensive using process characterization studies. Therefore, a simple and time-saving method is highly needed for the biopharmaceutical industry. Apoptosis is responsible for 80% of Chinese hamster...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-03-01
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| Series: | Bioengineering |
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| Online Access: | https://www.mdpi.com/2306-5354/10/3/357 |
| _version_ | 1797613435533393920 |
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| author | Shang Xiao Qiang Li Jinlong Jiang Chengxiao Huo Hao Chen Meijin Guo |
| author_facet | Shang Xiao Qiang Li Jinlong Jiang Chengxiao Huo Hao Chen Meijin Guo |
| author_sort | Shang Xiao |
| collection | DOAJ |
| description | Currently, the assessment of process robustness is often time-consuming, labor-intensive, and material-intensive using process characterization studies. Therefore, a simple and time-saving method is highly needed for the biopharmaceutical industry. Apoptosis is responsible for 80% of Chinese hamster ovary (CHO) cell deaths and affects the robustness of the cell culture process. This study’s results showed that a more robust process can support cells to tolerate apoptosis for a longer time, suggesting that the robustness of the process could be judged by the ability of cells to resist apoptosis. Therefore, it is necessary to establish a rapid method to detect the apoptosis of CHO cells. In trying to establish a new method for detecting apoptosis in large-scale cell cultures, glucose withdrawal was studied, and the results showed that CHO cells began to apoptose after glucose was consumed. Then, the concentration of extracellular potassium increased, and a prolongation of apoptosis time was observed. Further study results showed that the process with poor robustness was associated with a higher proportion of apoptosis and extracellular potassium concentration, so potassium could be used as a biochemical index of apoptosis. The strategy we present may be used to expedite the assessment of process robustness to obtain a robust cell culture process for other biologics. |
| first_indexed | 2024-03-11T06:55:46Z |
| format | Article |
| id | doaj.art-bb4ce1eb669a4f63a2506762b3866689 |
| institution | Directory Open Access Journal |
| issn | 2306-5354 |
| language | English |
| last_indexed | 2024-03-11T06:55:46Z |
| publishDate | 2023-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Bioengineering |
| spelling | doaj.art-bb4ce1eb669a4f63a2506762b38666892023-11-17T09:40:09ZengMDPI AGBioengineering2306-53542023-03-0110335710.3390/bioengineering10030357Rapid Identification of Chinese Hamster Ovary Cell Apoptosis and Its Potential Role in Process Robustness AssessmentShang Xiao0Qiang Li1Jinlong Jiang2Chengxiao Huo3Hao Chen4Meijin Guo5State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, ChinaState Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, ChinaState Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, ChinaShanghai Biological Products Research Institute Co., Ltd., Shanghai 200051, ChinaShanghai Biological Products Research Institute Co., Ltd., Shanghai 200051, ChinaState Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, ChinaCurrently, the assessment of process robustness is often time-consuming, labor-intensive, and material-intensive using process characterization studies. Therefore, a simple and time-saving method is highly needed for the biopharmaceutical industry. Apoptosis is responsible for 80% of Chinese hamster ovary (CHO) cell deaths and affects the robustness of the cell culture process. This study’s results showed that a more robust process can support cells to tolerate apoptosis for a longer time, suggesting that the robustness of the process could be judged by the ability of cells to resist apoptosis. Therefore, it is necessary to establish a rapid method to detect the apoptosis of CHO cells. In trying to establish a new method for detecting apoptosis in large-scale cell cultures, glucose withdrawal was studied, and the results showed that CHO cells began to apoptose after glucose was consumed. Then, the concentration of extracellular potassium increased, and a prolongation of apoptosis time was observed. Further study results showed that the process with poor robustness was associated with a higher proportion of apoptosis and extracellular potassium concentration, so potassium could be used as a biochemical index of apoptosis. The strategy we present may be used to expedite the assessment of process robustness to obtain a robust cell culture process for other biologics.https://www.mdpi.com/2306-5354/10/3/357CHOapoptosisglucose withdrawalrobustnessprocess characterization |
| spellingShingle | Shang Xiao Qiang Li Jinlong Jiang Chengxiao Huo Hao Chen Meijin Guo Rapid Identification of Chinese Hamster Ovary Cell Apoptosis and Its Potential Role in Process Robustness Assessment Bioengineering CHO apoptosis glucose withdrawal robustness process characterization |
| title | Rapid Identification of Chinese Hamster Ovary Cell Apoptosis and Its Potential Role in Process Robustness Assessment |
| title_full | Rapid Identification of Chinese Hamster Ovary Cell Apoptosis and Its Potential Role in Process Robustness Assessment |
| title_fullStr | Rapid Identification of Chinese Hamster Ovary Cell Apoptosis and Its Potential Role in Process Robustness Assessment |
| title_full_unstemmed | Rapid Identification of Chinese Hamster Ovary Cell Apoptosis and Its Potential Role in Process Robustness Assessment |
| title_short | Rapid Identification of Chinese Hamster Ovary Cell Apoptosis and Its Potential Role in Process Robustness Assessment |
| title_sort | rapid identification of chinese hamster ovary cell apoptosis and its potential role in process robustness assessment |
| topic | CHO apoptosis glucose withdrawal robustness process characterization |
| url | https://www.mdpi.com/2306-5354/10/3/357 |
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