Promotion of cytoplasmic vacuolation-mediated cell death of human prostate cancer PC-3 cells by oxidative stress induced by daucusol, a new guaiane-type sesquiterpenoid from Daucus carota L.
We investigated the antitumor activity of daucusol (DS) derived from Daucus carota L. in PC-3, A549 and HeLa cell lines by the MTT assay. Optical microscopy revealed that exposure of PC-3 cells to DS resulted in cytoplasmic vacuolation. Flow cytometry analysis of the phase of the cell cycle did not...
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| Format: | Article |
| Language: | English |
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University of Belgrade, University of Novi Sad
2017-01-01
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| Series: | Archives of Biological Sciences |
| Subjects: | |
| Online Access: | http://www.doiserbia.nb.rs/img/doi/0354-4664/2017/0354-46641600123H.pdf |
| _version_ | 1811197276624781312 |
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| author | Han Haote Liu Na Zhang Lin Gong Minghua Cao Ming Li Baoguo Kaisa Sulaiman Yu Xiuying Tian Jingkui |
| author_facet | Han Haote Liu Na Zhang Lin Gong Minghua Cao Ming Li Baoguo Kaisa Sulaiman Yu Xiuying Tian Jingkui |
| author_sort | Han Haote |
| collection | DOAJ |
| description | We investigated the antitumor activity of daucusol (DS) derived from Daucus carota L. in PC-3, A549 and HeLa cell lines by the MTT assay. Optical microscopy revealed that exposure of PC-3 cells to DS resulted in cytoplasmic vacuolation. Flow cytometry analysis of the phase of the cell cycle did not reveal a sub-G1 peak, and no caspase-dependent activation was observed after DS treatment. The levels of endoplasmic reticulum (ER) stress biomarkers, LC3B-II and ubiquitinated proteins were increased. It was also observed that oxidative stress played an important role in the activation of the cytoplasmic vacuolation-mediated cell-death pathway. In vivo, DS inhibited tumor growth in nude mice by 39.13% compared to the vehicle. Protein expression in the tumor tissue was consistent with their expression in vitro. Our findings indicate that DS induced cytoplasmic vacuolation-mediated death in PC-3 cells by triggering oxidative stress and suggest that targeting this pathway could serve as a novel therapeutic approach for prostate cancer. |
| first_indexed | 2024-04-12T01:11:24Z |
| format | Article |
| id | doaj.art-bb4de98667f845fca28dd84209cb9a3b |
| institution | Directory Open Access Journal |
| issn | 0354-4664 1821-4339 |
| language | English |
| last_indexed | 2024-04-12T01:11:24Z |
| publishDate | 2017-01-01 |
| publisher | University of Belgrade, University of Novi Sad |
| record_format | Article |
| series | Archives of Biological Sciences |
| spelling | doaj.art-bb4de98667f845fca28dd84209cb9a3b2022-12-22T03:54:05ZengUniversity of Belgrade, University of Novi SadArchives of Biological Sciences0354-46641821-43392017-01-0169348148910.2298/ABS160902123H0354-46641600123HPromotion of cytoplasmic vacuolation-mediated cell death of human prostate cancer PC-3 cells by oxidative stress induced by daucusol, a new guaiane-type sesquiterpenoid from Daucus carota L.Han Haote0Liu Na1Zhang Lin2Gong Minghua3Cao Ming4Li Baoguo5Kaisa Sulaiman6Yu Xiuying7Tian Jingkui8Zhejiang University, Department of Biomedical Engineering, The Key Laboratory of Biomedical Engineering, Ministry of Education, Hangzhou, P.R. ChinaShandong University of Traditional Chinese Medicine, Jinan, P.R. ChinaZhejiang University, Department of Biomedical Engineering, The Key Laboratory of Biomedical Engineering, Ministry of Education, Hangzhou, P.R. ChinaShandong University of Traditional Chinese Medicine, Jinan, P.R. ChinaWenzhou MedicalUniversity, Department of Pharmacology, Wenzhou, P.R. ChinaShandong University of Traditional Chinese Medicine, Jinan, P.R. ChinaThe Xinjiang Uygur Autonomous Region National Institute of traditional Chinese Medicine, Urumchi, P.R. ChinaZhejiang University, Department of Biomedical Engineering, The Key Laboratory of Biomedical Engineering, Ministry of Education, Hangzhou, P.R. ChinaZhejiang University, Department of Biomedical Engineering, The Key Laboratory of Biomedical Engineering, Ministry of Education, Hangzhou, P.R. ChinaWe investigated the antitumor activity of daucusol (DS) derived from Daucus carota L. in PC-3, A549 and HeLa cell lines by the MTT assay. Optical microscopy revealed that exposure of PC-3 cells to DS resulted in cytoplasmic vacuolation. Flow cytometry analysis of the phase of the cell cycle did not reveal a sub-G1 peak, and no caspase-dependent activation was observed after DS treatment. The levels of endoplasmic reticulum (ER) stress biomarkers, LC3B-II and ubiquitinated proteins were increased. It was also observed that oxidative stress played an important role in the activation of the cytoplasmic vacuolation-mediated cell-death pathway. In vivo, DS inhibited tumor growth in nude mice by 39.13% compared to the vehicle. Protein expression in the tumor tissue was consistent with their expression in vitro. Our findings indicate that DS induced cytoplasmic vacuolation-mediated death in PC-3 cells by triggering oxidative stress and suggest that targeting this pathway could serve as a novel therapeutic approach for prostate cancer.http://www.doiserbia.nb.rs/img/doi/0354-4664/2017/0354-46641600123H.pdfdaucusolcytoplasmic vacuolationoxidative stressendoplasmic reticulum stressprostate cancer |
| spellingShingle | Han Haote Liu Na Zhang Lin Gong Minghua Cao Ming Li Baoguo Kaisa Sulaiman Yu Xiuying Tian Jingkui Promotion of cytoplasmic vacuolation-mediated cell death of human prostate cancer PC-3 cells by oxidative stress induced by daucusol, a new guaiane-type sesquiterpenoid from Daucus carota L. Archives of Biological Sciences daucusol cytoplasmic vacuolation oxidative stress endoplasmic reticulum stress prostate cancer |
| title | Promotion of cytoplasmic vacuolation-mediated cell death of human prostate cancer PC-3 cells by oxidative stress induced by daucusol, a new guaiane-type sesquiterpenoid from Daucus carota L. |
| title_full | Promotion of cytoplasmic vacuolation-mediated cell death of human prostate cancer PC-3 cells by oxidative stress induced by daucusol, a new guaiane-type sesquiterpenoid from Daucus carota L. |
| title_fullStr | Promotion of cytoplasmic vacuolation-mediated cell death of human prostate cancer PC-3 cells by oxidative stress induced by daucusol, a new guaiane-type sesquiterpenoid from Daucus carota L. |
| title_full_unstemmed | Promotion of cytoplasmic vacuolation-mediated cell death of human prostate cancer PC-3 cells by oxidative stress induced by daucusol, a new guaiane-type sesquiterpenoid from Daucus carota L. |
| title_short | Promotion of cytoplasmic vacuolation-mediated cell death of human prostate cancer PC-3 cells by oxidative stress induced by daucusol, a new guaiane-type sesquiterpenoid from Daucus carota L. |
| title_sort | promotion of cytoplasmic vacuolation mediated cell death of human prostate cancer pc 3 cells by oxidative stress induced by daucusol a new guaiane type sesquiterpenoid from daucus carota l |
| topic | daucusol cytoplasmic vacuolation oxidative stress endoplasmic reticulum stress prostate cancer |
| url | http://www.doiserbia.nb.rs/img/doi/0354-4664/2017/0354-46641600123H.pdf |
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