Correlative Analysis of Immunoreactivity in Confocal Laser-Scanning Microscopy and Scanning Electron Microscopy with Focused Ion Beam Milling

Correlative Analysis of Immunoreactivity in Confocal Laser-Scanning Microscopy and Scanning Electron Microscopy with Focused Ion Beam Milling

Three-dimensional reconstruction of ultrastructure of rat brain with minimal effort has recently been realized by scanning electron microscopy combined with focused ion beam milling (FIB-SEM). Because application of immunohistochemical staining to electron microscopy has a great advantage in that m...

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Bibliographic Details
Main Authors: Takahiro eSonomura, Takahiro eFuruta, Ikuko eNakatani, Yo eYamamoto, Tomo eUnzai, Wakoto eMatsuda, Haruki eIwai, Atsushi eYamanaka, Masanori eUemura, Takehsi eKaneko
Format: Article
Language:English
Published: Frontiers Media S.A. 2013-02-01
Series:Frontiers in Neural Circuits
Subjects:
Neostriatum
synapse
neural circuit
immunocytochemistry
Scanning electron microscopy
confocal laser-scanning microscope
Online Access:http://journal.frontiersin.org/Journal/10.3389/fncir.2013.00026/full

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