Observing Fluorescent Probes in Living Cells using a Low-Cost LED Flashlight Retrofitted to a Common Vintage Light Microscope
While the application of molecular biological techniques based upon fluorescent probes has rapidly expanded over recent decades, the equipment cost of fluorescent microscopy has largely prevented its adoption in the college and high school classroom. We offer a simple solution to this problem by des...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
| Published: |
American Society for Microbiology
2013-01-01
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| Series: | Journal of Microbiology & Biology Education |
| Online Access: | https://journals.asm.org/doi/10.1128/jmbe.v14i1.530 |
| _version_ | 1818834360611110912 |
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| author | G. A. Babbitt C. A. Hanzlik K. N. Busse |
| author_facet | G. A. Babbitt C. A. Hanzlik K. N. Busse |
| author_sort | G. A. Babbitt |
| collection | DOAJ |
| description | While the application of molecular biological techniques based upon fluorescent probes has rapidly expanded over recent decades, the equipment cost of fluorescent microscopy has largely prevented its adoption in the college and high school classroom. We offer a simple solution to this problem by describing in detail how to build with simple tools, a fluorescent microscope using a common brand of colored LED flashlights and second-hand components of vintage Nikon microscopes. This extremely low cost solution is qualitatively compared to an expensive modern Zeiss system. |
| first_indexed | 2024-12-19T02:33:35Z |
| format | Article |
| id | doaj.art-bb4f83327de8481abf6dc762b1b00f55 |
| institution | Directory Open Access Journal |
| issn | 1935-7877 1935-7885 |
| language | English |
| last_indexed | 2024-12-19T02:33:35Z |
| publishDate | 2013-01-01 |
| publisher | American Society for Microbiology |
| record_format | Article |
| series | Journal of Microbiology & Biology Education |
| spelling | doaj.art-bb4f83327de8481abf6dc762b1b00f552022-12-21T20:39:33ZengAmerican Society for MicrobiologyJournal of Microbiology & Biology Education1935-78771935-78852013-01-0114112112410.1128/jmbe.v14i1.530Observing Fluorescent Probes in Living Cells using a Low-Cost LED Flashlight Retrofitted to a Common Vintage Light MicroscopeG. A. Babbitt0C. A. Hanzlik1K. N. Busse2T.H. Gosnell School of Life Sciences, Rochester Institute of Technology, Rochester, NY 14623T.H. Gosnell School of Life Sciences, Rochester Institute of Technology, Rochester, NY 14623Biomedical Engineering, Kate Gleason College of Engineering, Rochester Institute of Technology, Rochester, NY 14623While the application of molecular biological techniques based upon fluorescent probes has rapidly expanded over recent decades, the equipment cost of fluorescent microscopy has largely prevented its adoption in the college and high school classroom. We offer a simple solution to this problem by describing in detail how to build with simple tools, a fluorescent microscope using a common brand of colored LED flashlights and second-hand components of vintage Nikon microscopes. This extremely low cost solution is qualitatively compared to an expensive modern Zeiss system.https://journals.asm.org/doi/10.1128/jmbe.v14i1.530 |
| spellingShingle | G. A. Babbitt C. A. Hanzlik K. N. Busse Observing Fluorescent Probes in Living Cells using a Low-Cost LED Flashlight Retrofitted to a Common Vintage Light Microscope Journal of Microbiology & Biology Education |
| title | Observing Fluorescent Probes in Living Cells using a Low-Cost LED Flashlight Retrofitted to a Common Vintage Light Microscope |
| title_full | Observing Fluorescent Probes in Living Cells using a Low-Cost LED Flashlight Retrofitted to a Common Vintage Light Microscope |
| title_fullStr | Observing Fluorescent Probes in Living Cells using a Low-Cost LED Flashlight Retrofitted to a Common Vintage Light Microscope |
| title_full_unstemmed | Observing Fluorescent Probes in Living Cells using a Low-Cost LED Flashlight Retrofitted to a Common Vintage Light Microscope |
| title_short | Observing Fluorescent Probes in Living Cells using a Low-Cost LED Flashlight Retrofitted to a Common Vintage Light Microscope |
| title_sort | observing fluorescent probes in living cells using a low cost led flashlight retrofitted to a common vintage light microscope |
| url | https://journals.asm.org/doi/10.1128/jmbe.v14i1.530 |
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