Investigations on the role of ionic liquid on the physicochemical characteristics and toxicological consequences of liposomes
Effect of 1-butyl-3-methylimidazolium chloride ionic liquid ([bmim]Cl, IL) on the monolayer/bilayer of either soy-phosphatidylcholine (SPC) or hydrogenated soy-phosphatidylcholine (HSPC), in combination with 30 mol% cholesterol (Chol), were investigated. Impact of IL on monolayers were explored by...
| Main Authors: | , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2022-07-01
|
| Series: | JCIS Open |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666934X22000083 |
| _version_ | 1811258249104588800 |
|---|---|
| author | Manas Kumar Mandal Emili Manna Habiba Sultana Manas Barai Kartik Chandra Guchhait Chandradipa Ghosh Anuttam Patra Kaushik Nag Shin-ichi Yusa Amiya Kumar Panda |
| author_facet | Manas Kumar Mandal Emili Manna Habiba Sultana Manas Barai Kartik Chandra Guchhait Chandradipa Ghosh Anuttam Patra Kaushik Nag Shin-ichi Yusa Amiya Kumar Panda |
| author_sort | Manas Kumar Mandal |
| collection | DOAJ |
| description | Effect of 1-butyl-3-methylimidazolium chloride ionic liquid ([bmim]Cl, IL) on the monolayer/bilayer of either soy-phosphatidylcholine (SPC) or hydrogenated soy-phosphatidylcholine (HSPC), in combination with 30 mol% cholesterol (Chol), were investigated. Impact of IL on monolayers were explored by measuring the surface pressure (π)-area (A) isotherm with a Langmuir-surface balance. Lift-off area (A0) of the monomolecular films gradually increased [A0(HSPC+IL) > A0(SPC+IL)], collapse pressures (πc) decreased and passed through minima [πc(HSPC+IL) > πc(SPC+IL)] with increasing IL concentration ([IL]). Minimum molecular area (Amin) increased monotonously and compression moduli (Cs−1) followed the sequence (HSPC+IL) > (SPC+IL) at a particular π with respect to [IL]. Dynamic light scattering studies were carried out to determine the hydrodynamic diameter (dh), zeta potential (Z.P.) and polydispersity index (PDI) values while fluorescence anisotropy studies, using 7-hydroxycoumarin and 1,6-diphenyl-1,3,5-hexatriene, could reveal the micro-viscosity of liposomes. Increased size and rigidity, induced by IL, suggest the formation of leak-proof, condensed liposomes. Disruption of vesicles induced by IL were observed from transmission electron microscopic (TEM) studies. IL induced disintegration of liposome and kinetics of subsequent formation of adsorbed monolayer were accomplished by surface pressure-time isotherms. IL-induced liposomes were substantially less toxic as revealed by MTT assay. These liposomes are considered to be safely used as effective and controlled drug delivery systems. |
| first_indexed | 2024-04-12T18:10:22Z |
| format | Article |
| id | doaj.art-bb57a541881649fd98771a1d33484c7a |
| institution | Directory Open Access Journal |
| issn | 2666-934X |
| language | English |
| last_indexed | 2024-04-12T18:10:22Z |
| publishDate | 2022-07-01 |
| publisher | Elsevier |
| record_format | Article |
| series | JCIS Open |
| spelling | doaj.art-bb57a541881649fd98771a1d33484c7a2022-12-22T03:21:50ZengElsevierJCIS Open2666-934X2022-07-016100050Investigations on the role of ionic liquid on the physicochemical characteristics and toxicological consequences of liposomesManas Kumar Mandal0Emili Manna1Habiba Sultana2Manas Barai3Kartik Chandra Guchhait4Chandradipa Ghosh5Anuttam Patra6Kaushik Nag7Shin-ichi Yusa8Amiya Kumar Panda9Department of Chemistry, Vidyasagar University, Midnapore, 721102, West Bengal, IndiaDepartment of Chemistry, Vidyasagar University, Midnapore, 721102, West Bengal, IndiaDepartment of Chemistry, Vidyasagar University, Midnapore, 721102, West Bengal, IndiaDepartment of Chemistry, Vidyasagar University, Midnapore, 721102, West Bengal, IndiaDepartment of Human Physiology, Vidyasagar University, Midnapore, 721102, West Bengal, IndiaDepartment of Human Physiology, Vidyasagar University, Midnapore, 721102, West Bengal, IndiaChemistry of Interfaces Group, Luleå University of Technology, SE-97187, Luleå, SwedenDepartment of Biochemistry, Memorial University of Newfoundland, CanadaDepartment of Applied Chemistry, Graduate School of Engineering, University of Hyogo, Hyogo, 671-2280, JapanDepartment of Chemistry, Vidyasagar University, Midnapore, 721102, West Bengal, India; Sadhu Ram Chand Murmu University of Jhargram, Jhargram, 721507, West Bengal, India; Corresponding author. Department of Chemistry, Vidyasagar University, Midnapore, 721102, West Bengal, India.Effect of 1-butyl-3-methylimidazolium chloride ionic liquid ([bmim]Cl, IL) on the monolayer/bilayer of either soy-phosphatidylcholine (SPC) or hydrogenated soy-phosphatidylcholine (HSPC), in combination with 30 mol% cholesterol (Chol), were investigated. Impact of IL on monolayers were explored by measuring the surface pressure (π)-area (A) isotherm with a Langmuir-surface balance. Lift-off area (A0) of the monomolecular films gradually increased [A0(HSPC+IL) > A0(SPC+IL)], collapse pressures (πc) decreased and passed through minima [πc(HSPC+IL) > πc(SPC+IL)] with increasing IL concentration ([IL]). Minimum molecular area (Amin) increased monotonously and compression moduli (Cs−1) followed the sequence (HSPC+IL) > (SPC+IL) at a particular π with respect to [IL]. Dynamic light scattering studies were carried out to determine the hydrodynamic diameter (dh), zeta potential (Z.P.) and polydispersity index (PDI) values while fluorescence anisotropy studies, using 7-hydroxycoumarin and 1,6-diphenyl-1,3,5-hexatriene, could reveal the micro-viscosity of liposomes. Increased size and rigidity, induced by IL, suggest the formation of leak-proof, condensed liposomes. Disruption of vesicles induced by IL were observed from transmission electron microscopic (TEM) studies. IL induced disintegration of liposome and kinetics of subsequent formation of adsorbed monolayer were accomplished by surface pressure-time isotherms. IL-induced liposomes were substantially less toxic as revealed by MTT assay. These liposomes are considered to be safely used as effective and controlled drug delivery systems.http://www.sciencedirect.com/science/article/pii/S2666934X22000083Ionic liquidSurface pressureMonolayersVesiclesTEMCytotoxicity |
| spellingShingle | Manas Kumar Mandal Emili Manna Habiba Sultana Manas Barai Kartik Chandra Guchhait Chandradipa Ghosh Anuttam Patra Kaushik Nag Shin-ichi Yusa Amiya Kumar Panda Investigations on the role of ionic liquid on the physicochemical characteristics and toxicological consequences of liposomes JCIS Open Ionic liquid Surface pressure Monolayers Vesicles TEM Cytotoxicity |
| title | Investigations on the role of ionic liquid on the physicochemical characteristics and toxicological consequences of liposomes |
| title_full | Investigations on the role of ionic liquid on the physicochemical characteristics and toxicological consequences of liposomes |
| title_fullStr | Investigations on the role of ionic liquid on the physicochemical characteristics and toxicological consequences of liposomes |
| title_full_unstemmed | Investigations on the role of ionic liquid on the physicochemical characteristics and toxicological consequences of liposomes |
| title_short | Investigations on the role of ionic liquid on the physicochemical characteristics and toxicological consequences of liposomes |
| title_sort | investigations on the role of ionic liquid on the physicochemical characteristics and toxicological consequences of liposomes |
| topic | Ionic liquid Surface pressure Monolayers Vesicles TEM Cytotoxicity |
| url | http://www.sciencedirect.com/science/article/pii/S2666934X22000083 |
| work_keys_str_mv | AT manaskumarmandal investigationsontheroleofionicliquidonthephysicochemicalcharacteristicsandtoxicologicalconsequencesofliposomes AT emilimanna investigationsontheroleofionicliquidonthephysicochemicalcharacteristicsandtoxicologicalconsequencesofliposomes AT habibasultana investigationsontheroleofionicliquidonthephysicochemicalcharacteristicsandtoxicologicalconsequencesofliposomes AT manasbarai investigationsontheroleofionicliquidonthephysicochemicalcharacteristicsandtoxicologicalconsequencesofliposomes AT kartikchandraguchhait investigationsontheroleofionicliquidonthephysicochemicalcharacteristicsandtoxicologicalconsequencesofliposomes AT chandradipaghosh investigationsontheroleofionicliquidonthephysicochemicalcharacteristicsandtoxicologicalconsequencesofliposomes AT anuttampatra investigationsontheroleofionicliquidonthephysicochemicalcharacteristicsandtoxicologicalconsequencesofliposomes AT kaushiknag investigationsontheroleofionicliquidonthephysicochemicalcharacteristicsandtoxicologicalconsequencesofliposomes AT shinichiyusa investigationsontheroleofionicliquidonthephysicochemicalcharacteristicsandtoxicologicalconsequencesofliposomes AT amiyakumarpanda investigationsontheroleofionicliquidonthephysicochemicalcharacteristicsandtoxicologicalconsequencesofliposomes |