Downregulation of immunoglobulin-like transcript-4 (ILT4) in patients with psoriatic arthritis.
The immunoglobulin-like transcript-4 (ILT4) is an inhibitory receptor that modulates the activity of innate immune agents. We determined the expression of ILT4 and analysed the relationship with the expression of costimulatory proteins and tumor necrosis factor-α (TNF-α) production in monocytes from...
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2014-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC3967997?pdf=render |
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| author | Alberto Bergamini Maria Sole Chimenti Eleonora Baffari Maria Domenica Guarino Gianfranco Gigliucci Carlo Perricone Roberto Perricone |
| author_facet | Alberto Bergamini Maria Sole Chimenti Eleonora Baffari Maria Domenica Guarino Gianfranco Gigliucci Carlo Perricone Roberto Perricone |
| author_sort | Alberto Bergamini |
| collection | DOAJ |
| description | The immunoglobulin-like transcript-4 (ILT4) is an inhibitory receptor that modulates the activity of innate immune agents. We determined the expression of ILT4 and analysed the relationship with the expression of costimulatory proteins and tumor necrosis factor-α (TNF-α) production in monocytes from patients with psoriatic arthritis (PsA) starting anti-TNF treatment.Peripheral blood monocytes from 15 healthy controls and from 16 patients with PsA were activated in vitro by CD40 ligand (CD40L) and analyzed for ILT4, CD40, CD80 and CD86 expression, and spontaneous lipopolysaccharide (LPS)-induced TNF-α production by flow cytometry, before and after treatment with adalimumab.The percentage of ILT4-negative monocytes was greater in PsA patients compared to controls and negatively correlated with DAS44. Normal monocytes treated with sera of PsA patients showed a reduced expression of ILT4 compared with monocytes exposed to sera from controls. CD40, CD80 and CD86 expression was higher in patients compared to controls. Both spontaneous and LPS-induced TNF-α production was restricted to ILT4-negative monocytes and was greater in PsA patients compared to controls. Finally, twelve weeks-treatment with adalimumab resulted in a significant increase of ILT4 expression and a decrease of costimulatory molecules expression in PsA patients, compared to pre-therapy levels.These data support the possibility that changes in the immunophenotype of monocytes play a role in the pathogenesis of PSA. Thus, modulation of the expression of ILT4 may represent an enticing new therapeutic target. |
| first_indexed | 2024-04-13T05:46:15Z |
| format | Article |
| id | doaj.art-bb5925ea60464b57b85aa8a43f0f6385 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-04-13T05:46:15Z |
| publishDate | 2014-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-bb5925ea60464b57b85aa8a43f0f63852022-12-22T02:59:57ZengPublic Library of Science (PLoS)PLoS ONE1932-62032014-01-0193e9201810.1371/journal.pone.0092018Downregulation of immunoglobulin-like transcript-4 (ILT4) in patients with psoriatic arthritis.Alberto BergaminiMaria Sole ChimentiEleonora BaffariMaria Domenica GuarinoGianfranco GigliucciCarlo PerriconeRoberto PerriconeThe immunoglobulin-like transcript-4 (ILT4) is an inhibitory receptor that modulates the activity of innate immune agents. We determined the expression of ILT4 and analysed the relationship with the expression of costimulatory proteins and tumor necrosis factor-α (TNF-α) production in monocytes from patients with psoriatic arthritis (PsA) starting anti-TNF treatment.Peripheral blood monocytes from 15 healthy controls and from 16 patients with PsA were activated in vitro by CD40 ligand (CD40L) and analyzed for ILT4, CD40, CD80 and CD86 expression, and spontaneous lipopolysaccharide (LPS)-induced TNF-α production by flow cytometry, before and after treatment with adalimumab.The percentage of ILT4-negative monocytes was greater in PsA patients compared to controls and negatively correlated with DAS44. Normal monocytes treated with sera of PsA patients showed a reduced expression of ILT4 compared with monocytes exposed to sera from controls. CD40, CD80 and CD86 expression was higher in patients compared to controls. Both spontaneous and LPS-induced TNF-α production was restricted to ILT4-negative monocytes and was greater in PsA patients compared to controls. Finally, twelve weeks-treatment with adalimumab resulted in a significant increase of ILT4 expression and a decrease of costimulatory molecules expression in PsA patients, compared to pre-therapy levels.These data support the possibility that changes in the immunophenotype of monocytes play a role in the pathogenesis of PSA. Thus, modulation of the expression of ILT4 may represent an enticing new therapeutic target.http://europepmc.org/articles/PMC3967997?pdf=render |
| spellingShingle | Alberto Bergamini Maria Sole Chimenti Eleonora Baffari Maria Domenica Guarino Gianfranco Gigliucci Carlo Perricone Roberto Perricone Downregulation of immunoglobulin-like transcript-4 (ILT4) in patients with psoriatic arthritis. PLoS ONE |
| title | Downregulation of immunoglobulin-like transcript-4 (ILT4) in patients with psoriatic arthritis. |
| title_full | Downregulation of immunoglobulin-like transcript-4 (ILT4) in patients with psoriatic arthritis. |
| title_fullStr | Downregulation of immunoglobulin-like transcript-4 (ILT4) in patients with psoriatic arthritis. |
| title_full_unstemmed | Downregulation of immunoglobulin-like transcript-4 (ILT4) in patients with psoriatic arthritis. |
| title_short | Downregulation of immunoglobulin-like transcript-4 (ILT4) in patients with psoriatic arthritis. |
| title_sort | downregulation of immunoglobulin like transcript 4 ilt4 in patients with psoriatic arthritis |
| url | http://europepmc.org/articles/PMC3967997?pdf=render |
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